Protein WP_012992059.1 in Thermocrinis albus DSM 14484
Annotation: NCBI__GCF_000025605.1:WP_012992059.1
Length: 477 amino acids
Source: GCF_000025605.1 in NCBI
Candidate for 48 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-fucose catabolism | aldA | hi | lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) | 41% | 99% | 351.7 | phosphonoformaldehyde dehydrogenase | 40% | 330.1 |
| L-rhamnose catabolism | aldA | hi | lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) | 41% | 99% | 351.7 | phosphonoformaldehyde dehydrogenase | 40% | 330.1 |
| L-threonine catabolism | aldA | hi | lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) | 41% | 99% | 351.7 | phosphonoformaldehyde dehydrogenase | 40% | 330.1 |
| L-arginine catabolism | gabD | lo | Succinate-semialdehyde dehydrogenase, mitochondrial; At-SSADH1; Aldehyde dehydrogenase family 5 member F1; NAD(+)-dependent succinic semialdehyde dehydrogenase; Protein ENLARGED FIL EXPRESSING DOMAIN 1; EC 1.2.1.24 (characterized) | 38% | 88% | 315.8 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-citrulline catabolism | gabD | lo | Succinate-semialdehyde dehydrogenase, mitochondrial; At-SSADH1; Aldehyde dehydrogenase family 5 member F1; NAD(+)-dependent succinic semialdehyde dehydrogenase; Protein ENLARGED FIL EXPRESSING DOMAIN 1; EC 1.2.1.24 (characterized) | 38% | 88% | 315.8 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| putrescine catabolism | gabD | lo | Succinate-semialdehyde dehydrogenase, mitochondrial; At-SSADH1; Aldehyde dehydrogenase family 5 member F1; NAD(+)-dependent succinic semialdehyde dehydrogenase; Protein ENLARGED FIL EXPRESSING DOMAIN 1; EC 1.2.1.24 (characterized) | 38% | 88% | 315.8 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| 4-hydroxybenzoate catabolism | adh | lo | Aldehyde dehydrogenase 5, mitochondrial; EC 1.2.1.5 (characterized) | 39% | 91% | 304.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| 2'-deoxyinosine catabolism | adh | lo | Aldehyde dehydrogenase 5, mitochondrial; EC 1.2.1.5 (characterized) | 39% | 91% | 304.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| 2-deoxy-D-ribose catabolism | adh | lo | Aldehyde dehydrogenase 5, mitochondrial; EC 1.2.1.5 (characterized) | 39% | 91% | 304.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| ethanol catabolism | adh | lo | Aldehyde dehydrogenase 5, mitochondrial; EC 1.2.1.5 (characterized) | 39% | 91% | 304.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-threonine catabolism | adh | lo | Aldehyde dehydrogenase 5, mitochondrial; EC 1.2.1.5 (characterized) | 39% | 91% | 304.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| thymidine catabolism | adh | lo | Aldehyde dehydrogenase 5, mitochondrial; EC 1.2.1.5 (characterized) | 39% | 91% | 304.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-tryptophan catabolism | adh | lo | Aldehyde dehydrogenase 5, mitochondrial; EC 1.2.1.5 (characterized) | 39% | 91% | 304.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arginine catabolism | davD | lo | glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) | 36% | 97% | 292 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-citrulline catabolism | davD | lo | glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) | 36% | 97% | 292 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-lysine catabolism | davD | lo | glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) | 36% | 97% | 292 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-proline catabolism | davD | lo | glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) | 36% | 97% | 292 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arginine catabolism | patD | lo | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 36% | 96% | 282.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arginine catabolism | puuC | lo | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 36% | 96% | 282.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-citrulline catabolism | patD | lo | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 36% | 96% | 282.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-citrulline catabolism | puuC | lo | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 36% | 96% | 282.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| putrescine catabolism | patD | lo | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 36% | 96% | 282.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| putrescine catabolism | puuC | lo | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 36% | 96% | 282.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arabinose catabolism | xacF | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 35% | 97% | 280 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| D-galacturonate catabolism | dopDH | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 35% | 97% | 280 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| D-glucuronate catabolism | dopDH | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 35% | 97% | 280 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| D-xylose catabolism | dopDH | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 35% | 97% | 280 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| 4-hydroxybenzoate catabolism | praB | lo | 2-aminomuconic semialdehyde dehydrogenase; Aldehyde dehydrogenase 12; Aldehyde dehydrogenase family 8 member A1; EC 1.2.1.32 (characterized) | 34% | 98% | 275.8 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-tryptophan catabolism | nbaE | lo | 2-aminomuconic semialdehyde dehydrogenase; Aldehyde dehydrogenase 12; Aldehyde dehydrogenase family 8 member A1; EC 1.2.1.32 (characterized) | 34% | 98% | 275.8 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-tryptophan catabolism | praB | lo | 2-aminomuconic semialdehyde dehydrogenase; Aldehyde dehydrogenase 12; Aldehyde dehydrogenase family 8 member A1; EC 1.2.1.32 (characterized) | 34% | 98% | 275.8 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-phenylalanine catabolism | pad-dh | lo | phenylacetaldehyde dehydrogenase (EC 1.2.1.39) (characterized) | 35% | 97% | 273.1 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arginine catabolism | kauB | lo | gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) | 35% | 95% | 270.8 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-lysine catabolism | patD | lo | aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) | 35% | 99% | 257.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-valine catabolism | mmsA | lo | Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27) (characterized) | 33% | 96% | 244.6 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arabinose catabolism | aldA | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized) | 32% | 95% | 243 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| D-xylose catabolism | aldA | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized) | 32% | 95% | 243 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-isoleucine catabolism | iolA | lo | malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) | 33% | 96% | 238 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| myo-inositol catabolism | mmsA | lo | malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) | 33% | 96% | 238 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| propionate catabolism | iolA | lo | malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) | 33% | 96% | 238 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-threonine catabolism | iolA | lo | malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) | 33% | 96% | 238 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-valine catabolism | iolA | lo | malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) | 33% | 96% | 238 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arginine catabolism | putA | lo | 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; EC 1.2.1.88; L-glutamate gamma-semialdehyde dehydrogenase (uncharacterized) | 31% | 91% | 226.1 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arginine catabolism | rocA | lo | 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; EC 1.2.1.88; L-glutamate gamma-semialdehyde dehydrogenase (uncharacterized) | 31% | 91% | 226.1 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-citrulline catabolism | putA | lo | 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; EC 1.2.1.88; L-glutamate gamma-semialdehyde dehydrogenase (uncharacterized) | 31% | 91% | 226.1 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-citrulline catabolism | rocA | lo | 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; EC 1.2.1.88; L-glutamate gamma-semialdehyde dehydrogenase (uncharacterized) | 31% | 91% | 226.1 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-proline catabolism | putA | lo | 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; EC 1.2.1.88; L-glutamate gamma-semialdehyde dehydrogenase (uncharacterized) | 31% | 91% | 226.1 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-arginine catabolism | astD | lo | N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized) | 31% | 93% | 195.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
| L-citrulline catabolism | astD | lo | N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized) | 31% | 93% | 195.3 | lactaldehyde dehydrogenase (EC 1.2.1.22) | 41% | 351.7 |
Sequence Analysis Tools
View WP_012992059.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MLEKPLILGGERRSTGKWLDVVYPYTGEVIGRASLASEEDVHTAVERAIEGHKQMASLTP
YERYRILMKAAQLLEERAEVFARTLVLEVGKTIREARTEVQRALQTLIFSAEEAKRIGGE
VIPLDAHPNGKGKVGFYIREPVGIVGAITPFNFPLNLSMHKVAPALAAGNAVILKPSERT
PLTPIMLGELLLEAGVPPMAISVLPGFGDVGKAISTHPQIRVISFTGSRKVGDIITRQAG
IKKVVLELGSNSAVVVHKDGDITKAVQKVIAGGYAIAGQVCISVQRVFVHEEVYEKFLME
LTSQVEKLKVGDPMDESTDVGPMISVSEVERIQSWIDEAVSMGAQLVTGGVSCGDKVASI
LTPTVVSLVPSNAKLFREEAFAPVVVVNPYREVDEAISMVNDSDYGLQVGVFTRDIKVAW
EFIRGVQAGGVLINEGPNFRADHMPYGGVKQSGIGREGPRFAIEDYTEVKMVIFDLS
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory