Align Aldehyde dehydrogenase; Acetaldehyde dehydrogenase; EC 1.2.1.3 (characterized)
to candidate WP_011426932.1 RHE_RS19035 aldehyde dehydrogenase
Query= SwissProt::A1B4L2 (508 letters) >NCBI__GCF_000092045.1:WP_011426932.1 Length = 502 Score = 836 bits (2159), Expect = 0.0 Identities = 400/493 (81%), Positives = 443/493 (89%) Query: 16 PFEERYDNFIGGEWVAPVSGRYFTNTTPITGAEIGQIARSEAGDIELALDAAHAAKEKWG 75 PF+ +Y N+IGGEW PV G+YF N TP+TG ++ I RS+ DI LALDAAHAAKE WG Sbjct: 10 PFKLKYGNYIGGEWREPVEGKYFENLTPVTGGKLCDIPRSDEKDINLALDAAHAAKENWG 69 Query: 76 ATSPAERANIMLKIADRMERNLELLATAETWDNGKPIRETMAADLPLAIDHFRYFAGVLR 135 TS AER+NI++KIA RME LELLA AETWDNGKPIRETMAAD+PLAIDHFRYFA +R Sbjct: 70 RTSAAERSNILMKIAQRMEDRLELLAQAETWDNGKPIRETMAADIPLAIDHFRYFASCIR 129 Query: 136 AQEGSISQIDDDTVAYHFHEPLGVVGQIIPWNFPLLMACWKLAPAIAAGNCVVLKPAEQT 195 AQEGSI +ID DT+AYHFHEPLGVVGQIIPWNFP+LMA WKLAPA+AAGNCVVLKPAEQT Sbjct: 130 AQEGSIGEIDHDTIAYHFHEPLGVVGQIIPWNFPILMATWKLAPALAAGNCVVLKPAEQT 189 Query: 196 PAGIMVWANLIGDLLPPGVLNIVNGFGLEAGKPLASSNRIAKIAFTGETTTGRLIMQYAS 255 PA I++WA L+GDLLPPGVLNIVNGFG+EAGKPLA+S RIAKIAFTGETTTGRLIMQYAS Sbjct: 190 PASILLWAELVGDLLPPGVLNIVNGFGIEAGKPLATSPRIAKIAFTGETTTGRLIMQYAS 249 Query: 256 ENLIPVTLELGGKSPNIFFADVAREDDDFFDKALEGFTMFALNQGEVCTCPSRVLIQESI 315 +NLIPVTLELGGKSPNIFFADV EDDDF DKALEGF MFALNQGEVCTCPSR L+QESI Sbjct: 250 QNLIPVTLELGGKSPNIFFADVMAEDDDFLDKALEGFAMFALNQGEVCTCPSRALVQESI 309 Query: 316 YDKFMERAVQRVQAIKQGDPRESDTMIGAQASSEQKEKILSYLDIGKKEGAEVLTGGKAA 375 YD+FME+ V+RV+AIKQG+P + TMIGAQAS+EQ EKIL+YLDIGK+EGA+VL GG Sbjct: 310 YDRFMEKTVKRVEAIKQGNPLDPATMIGAQASTEQLEKILAYLDIGKQEGAQVLAGGSRN 369 Query: 376 DLGGELSGGYYIEPTIFRGNNKMRIFQEEIFGPVVSVTTFKDQAEALEIANDTLYGLGAG 435 DLGG+L+ GYY++PTIF+G+NKMRIFQEEIFGPVVSVTTFK + EALEIANDTLYGLGAG Sbjct: 370 DLGGDLANGYYVKPTIFKGHNKMRIFQEEIFGPVVSVTTFKTEKEALEIANDTLYGLGAG 429 Query: 436 VWSRDANTCYRMGRGIKAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKN 495 VWSRDAN CYR GR I+AGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMML+HYQQTKN Sbjct: 430 VWSRDANRCYRFGREIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKN 489 Query: 496 MLVSYSPKKLGFF 508 MLVSYSPK LGFF Sbjct: 490 MLVSYSPKALGFF 502 Lambda K H 0.319 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 917 Number of extensions: 27 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 508 Length of database: 502 Length adjustment: 34 Effective length of query: 474 Effective length of database: 468 Effective search space: 221832 Effective search space used: 221832 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory