GapMind for catabolism of small carbon sources

 

2'-deoxyinosine catabolism in Rhizobium etli CFN 42

Best path

nupA, nupB, nupC', bmpA, deoD, deoB, deoC, adh, acs

Rules

Overview: In the known pathway for deoxyinosine utilization, a phosphorylase forms deoxyribose 1-phosphate, phosphopentomutase forms deoxyribose 5-phosphate, and an aldolase produces 3-phosphoglycerate (an intermediate in glycolysis) and acetaldehyde (link). MetaCyc also describes a purine deoxyribonucleosidase (EC 3.2.2.M2), yielding deoxyribose, but this enzyme has not been linked to sequence, so it is not included in GapMind. This reaction might also occur non-specifically via ribonucleosidases. The fitness data for Paraburkholderia bryophila 376MFSha3.1 does suggest cytoplasmic hydrolysis of purine deoxynucleosides, but did not identify the deoxyribonucleosidase.

18 steps (14 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
nupA deoxyinosine ABC transporter, ATPase component RHE_RS00955 RHE_RS22535
nupB deoxyinosine ABC transporter, permease component 1 RHE_RS00960 RHE_RS22540
nupC' deoxyinosine ABC transporter, permease component 2 RHE_RS00965 RHE_RS22545
bmpA deoxyinosine ABC transporter, substrate-binding component RHE_RS00950
deoD deoxyinosine phosphorylase RHE_RS00975
deoB phosphopentomutase RHE_RS01005
deoC deoxyribose-5-phosphate aldolase RHE_RS00980
adh acetaldehyde dehydrogenase (not acylating) RHE_RS19035 RHE_RS20725
acs acetyl-CoA synthetase, AMP-forming RHE_RS21025 RHE_RS21035
Alternative steps:
ackA acetate kinase RHE_RS17585
ald-dh-CoA acetaldehyde dehydrogenase, acylating
H281DRAFT_01112 deoxynucleoside transporter, permease component 2 RHE_RS06205 RHE_RS26590
H281DRAFT_01113 deoxynucleoside transporter, ATPase component RHE_RS25530 RHE_RS03100
H281DRAFT_01114 deoxynucleoside transporter, substrate-binding component RHE_RS25535
H281DRAFT_01115 deoxynucleoside transporter, permease component 1
nupC deoxyinosine:H+ symporter NupC
nupG deoxyinosine permease NupG/XapB
pta phosphate acetyltransferase RHE_RS01970 RHE_RS12030

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory