Alignments for a candidate for liuD in Rhizobium etli CFN 42

GapMind for catabolism of small carbon sources

Alignments for a candidate for liuD in Rhizobium etli CFN 42

Align Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) (characterized)
to candidate WP_011425493.1 RHE_RS11415 acyl-CoA carboxylase subunit beta

Query= reanno::psRCH2:GFF1050
         (535 letters)



>NCBI__GCF_000092045.1:WP_011425493.1
          Length = 510

 Score =  256 bits (654), Expect = 1e-72
 Identities = 179/513 (34%), Positives = 252/513 (49%), Gaps = 30/513 (5%)

Query: 22  MLEQVNDLRALLGRVSEGGGATAQQRHVSRGKLLVRERIDTLLDAGSAFLELAPLAAHEV 81
           M E + +L    G    GGG    +    RGKL  RERID  LD GS F E      H  
Sbjct: 1   MKEILEELERRRGIARLGGGKARIEAQHKRGKLTARERIDLFLDEGS-FEEFDMFVEHRS 59

Query: 82  --YGED---VAAAGVVAGIGRVEGIECMIIANDATVKGGTYYPLTVKKHLRAQTVARENR 136
             +G D   +A  GVV G G V G    + A D TV GG+      +K ++ Q +A +NR
Sbjct: 60  TDFGMDKSRIAGDGVVTGWGTVNGRTVFVFAKDFTVFGGSLSEAHAEKIMKVQDMALKNR 119

Query: 137 LPCIYLVDSGGANLPRQDEVFPDREHFGRIFFNQANMSAMG-IPQIAVVMGSCTAGGAYV 195
            P + + D+GGA +    E       +  +F  Q N+ A G IPQI+V+MG C  G  Y 
Sbjct: 120 APIVGIYDAGGARI---QEGVAALGGYAEVF--QRNVLASGVIPQISVIMGPCAGGDVYS 174

Query: 196 PAMADETIMVRNQATIFLAGPPLVKAATGEVVTAEELGGADVHCKTSGVADHYAENDEHA 255
           PAM D   MVR+ + +F+ GP +VK  T E VTAEELGGA VH   S +AD   +ND   
Sbjct: 175 PAMTDFIFMVRDTSYMFVTGPDVVKTVTNETVTAEELGGAVVHTVRSSIADGAYDNDVET 234

Query: 256 LSIARRCVANLNWRKLGQLQTREPRAPLYAAD-ELYGVIPAQAKQPYDVREVIARLVDGS 314
           L   RR +  L       L   E    +   D  L  ++PA A +PYD++E+I ++ D  
Sbjct: 235 LLQVRRLIDFLPLSNSAPLPEIECYQSVTEIDMSLDTLVPASANKPYDIKELIRKVADEG 294

Query: 315 EFDEFKALFGTTLVCGFAHLHGYPIAILANN-----GILFAEAAQKGAHFIELACQRGIP 369
           +F E +  F   +VCGF  + G  +  +AN      G+L ++A++K A F+       IP
Sbjct: 295 DFFEIQESFAKNIVCGFGRVEGSTVGFVANQPMVLAGVLDSDASRKAARFVRFCDCFNIP 354

Query: 370 LLFLQNITGFMVGQKYEAGGIAKHGAKLVTAVACAQVPKFTVLIGGSFGAGNYGMCGRAY 429
           ++   ++ GF+ G   E GG+ KHGAKL+ A A A VPK TV+   +FG     M  +  
Sbjct: 355 IVTFVDVPGFLPGTAQEYGGLIKHGAKLLFAYAEATVPKLTVITRKAFGGAYDVMASKHL 414

Query: 430 DPRFLWMWPNARIAVMGGEQAAGVLAQVKQEQSERAGKSLGDDEVAAIKQPILEQYERQG 489
                + WP A+IAVMG + A  ++ +          K + D E  A    + E  +R  
Sbjct: 415 RGDLNYAWPTAQIAVMGAKGAVEIIFR----------KDIADPEKIAAHTKMYE--DRFL 462

Query: 490 HPYYSSARLWDDGVIDPAQTREVLGLALSAALN 522
            P+ ++ R + D VI P  TR  L   L    N
Sbjct: 463 SPFVAAERGYIDEVIMPHSTRRRLARGLKMLRN 495


Lambda     K      H
   0.321    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 671
Number of extensions: 42
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 535
Length of database: 510
Length adjustment: 35
Effective length of query: 500
Effective length of database: 475
Effective search space:   237500
Effective search space used:   237500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources