Alignments for a candidate for iolA in Rhizobium etli CFN 42

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolA in Rhizobium etli CFN 42

Align malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)
to candidate WP_011424092.1 RHE_RS03695 CoA-acylating methylmalonate-semialdehyde dehydrogenase

Query= reanno::Smeli:SMc00781
         (498 letters)



>NCBI__GCF_000092045.1:WP_011424092.1
          Length = 498

 Score =  902 bits (2331), Expect = 0.0
 Identities = 433/498 (86%), Positives = 472/498 (94%)

Query: 1   MYELGHFIDGKRVAGTSGRVSNIFNPATGEVQGTVALASDADLAAAVESAKAAQPKWAAT 60
           M E+GHFI GK VAGTSGRVSN++NPATGEVQ TVALAS  +L AAVE+AKAAQPKWAAT
Sbjct: 1   MREIGHFIGGKEVAGTSGRVSNVYNPATGEVQATVALASVEELRAAVENAKAAQPKWAAT 60

Query: 61  NPQRRARVFMKFVQLLNDNMNELAEMLSREHGKTIDDAKGDIVRGLEVCEFVIGIPHLQK 120
           NPQRRARVF KFV+LLN +M+ELAEMLS+EHGKTI+DAKGD++RGLEVCEFV GIPHL K
Sbjct: 61  NPQRRARVFFKFVELLNKHMDELAEMLSKEHGKTIEDAKGDVIRGLEVCEFVCGIPHLAK 120

Query: 121 SEFTEGAGPGIDMYSIRQPVGIGAGITPFNFPGMIPMWMFAPAIACGNAFILKPSERDPS 180
            EFTEGAGP IDMYSIRQPVGIGAGITPFNFPGMIPMWMFAPAIACGNAFILKPSERDPS
Sbjct: 121 GEFTEGAGPAIDMYSIRQPVGIGAGITPFNFPGMIPMWMFAPAIACGNAFILKPSERDPS 180

Query: 181 VPIRLAELMIEAGLPAGILNVVNGDKGAVDAILTHPDIAAVSFVGSTPIARYVYGTAAMN 240
           +PIRLAELMIEAGLPAGILNVVNGDKGAVDAILT PDI AVSFVGSTPIARYVYGTAAMN
Sbjct: 181 LPIRLAELMIEAGLPAGILNVVNGDKGAVDAILTDPDIGAVSFVGSTPIARYVYGTAAMN 240

Query: 241 GKRAQCFGGAKNHMIIMPDADLDQAANALIGAGYGSAGERCMAISVAVPVGEETANRLID 300
           GKRAQCFGGAKNHMIIMPDAD+DQA NAL+GAGYGSAGERCMAISVAVPVG+ETANRL++
Sbjct: 241 GKRAQCFGGAKNHMIIMPDADMDQAVNALMGAGYGSAGERCMAISVAVPVGKETANRLVE 300

Query: 301 KLVPMVESLRIGPYTDEKADMGPVVTKEAEQRIRSLIDSGIEQGAKLVVDGRDFKLQGYE 360
            L P +ESLRIGPYTD+KADMGP+VTK+A  R+R LID G+E+GAKLVVDGRDFKLQGYE
Sbjct: 301 MLAPKIESLRIGPYTDDKADMGPLVTKDAYTRVRGLIDRGVEEGAKLVVDGRDFKLQGYE 360

Query: 361 NGHFIGGCLFDDVTPDMDIYKTEIFGPVLSVVRARNYEEALSLPMKHEYGNGVAIYTRDG 420
           +G+F+GGCLFD VTP+MDIYKTEIFGPVLSVVRA+NYEEALSLPMKHEYGNGVAIYTRDG
Sbjct: 361 DGYFVGGCLFDHVTPEMDIYKTEIFGPVLSVVRAKNYEEALSLPMKHEYGNGVAIYTRDG 420

Query: 421 DAARDFASRINIGMVGVNVPIPVPLAYHSFGGWKSSSFGDLNQHGTDSIKFWTRTKTITS 480
           DAARDFASR+NIGM+G+NVPIPVPLAYHSFGGWK+SSFGDLNQHGTDSIKFWT+TKT+T+
Sbjct: 421 DAARDFASRVNIGMIGINVPIPVPLAYHSFGGWKASSFGDLNQHGTDSIKFWTKTKTVTA 480

Query: 481 RWPSGIKDGAEFSIPTMR 498
           RWPSGIK GAEF +PTM+
Sbjct: 481 RWPSGIKSGAEFVMPTMK 498


Lambda     K      H
   0.319    0.137    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 934
Number of extensions: 26
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 498
Length of database: 498
Length adjustment: 34
Effective length of query: 464
Effective length of database: 464
Effective search space:   215296
Effective search space used:   215296
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources