GapMind for catabolism of small carbon sources

 

Alignments for a candidate for AZOBR_RS08245 in Arcobacter nitrofigilis DSM 7299

Align Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate WP_013134865.1 ARNIT_RS05295 urea ABC transporter ATP-binding protein UrtD

Query= uniprot:G8ALJ0
         (294 letters)



>NCBI__GCF_000092245.1:WP_013134865.1
          Length = 262

 Score =  124 bits (311), Expect = 2e-33
 Identities = 84/260 (32%), Positives = 140/260 (53%), Gaps = 19/260 (7%)

Query: 11  LLTVEHLTMRFGGLVAVNDVSFSANNGEITAIIGPNGAGKTTLFNCITGFYTPTVGRLTL 70
           +L V+ +++ F G  A+N++SFS N GE+  IIG NGAGK+T+ + +TG   P  G +  
Sbjct: 21  ILLVDGVSVSFDGFKALNNLSFSINYGELRCIIGANGAGKSTMMDVVTGKTRPDEGEVIF 80

Query: 71  RHADGKEFLLERMPGYRISQKASVARTFQNIRLFGGMSVLENLIVA-QHNKLIRASGFSI 129
               G+   L  +    IS+   + R FQ   +F   SV ENL +A + +K    + FS 
Sbjct: 81  ----GEAVNLLELDEPTISE-IGIGRKFQKPTVFQNHSVFENLELAMKDDKRFFKTLFS- 134

Query: 130 AGLLGLPSYTRTEREAVDLAKYWLDRVRLLEFADWEAGNLPYGAQRRLEIARAMCTEPVM 189
                     +   E  D  +  +  + L E  + +AG L +G ++ LEI   +  EP +
Sbjct: 135 ----------KLRSEQKDRIEETMKLIGLKELYNLDAGILSHGQKQWLEIGMLIMQEPKL 184

Query: 190 LCLDEPAAGLNPRESGELADLLTYIRDEHKIGVLLIEHDMSVVMTISDHVVVLDYGRKIS 249
           L +DEP AG+ P+E  + A++LT +  E+   V+++EHDM  + +I+  V VL  G  ++
Sbjct: 185 LLVDEPVAGMTPQEVEKTAEILTSLSKEN--AVVVVEHDMEFIRSIASKVTVLHEGSVLA 242

Query: 250 DGDPAFVKNDPAVIRAYLGE 269
           +G+   ++N+  V + YLGE
Sbjct: 243 EGNMDAIQNNEKVRKVYLGE 262


Lambda     K      H
   0.319    0.137    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 172
Number of extensions: 7
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 294
Length of database: 262
Length adjustment: 25
Effective length of query: 269
Effective length of database: 237
Effective search space:    63753
Effective search space used:    63753
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory