Alignments for a candidate for adh in Paraburkholderia atlantica CCGE1002

GapMind for catabolism of small carbon sources

Alignments for a candidate for adh in Paraburkholderia atlantica CCGE1002

Align Aldehyde dehydrogenase; Acetaldehyde dehydrogenase; EC 1.2.1.3 (characterized)
to candidate WP_013088770.1 BC1002_RS03875 aldehyde dehydrogenase family protein

Query= SwissProt::A1B4L2
         (508 letters)



>NCBI__GCF_000092885.1:WP_013088770.1
          Length = 508

 Score =  719 bits (1856), Expect = 0.0
 Identities = 341/493 (69%), Positives = 404/493 (81%)

Query: 16  PFEERYDNFIGGEWVAPVSGRYFTNTTPITGAEIGQIARSEAGDIELALDAAHAAKEKWG 75
           P+ +RY+N+IGG+WV PV G YF N +PI G    ++ RS A DI+LA+DAAHAA+ KW 
Sbjct: 16  PYRQRYENYIGGKWVPPVGGDYFDNVSPINGQPFCRVPRSSAADIDLAIDAAHAARRKWA 75

Query: 76  ATSPAERANIMLKIADRMERNLELLATAETWDNGKPIRETMAADLPLAIDHFRYFAGVLR 135
            TS  ERAN++L +A+RME+NL+LLA AET DNGKP+RETMAADLPLA+DHFRYFAG +R
Sbjct: 76  KTSVTERANLLLAVAERMEKNLKLLAVAETIDNGKPLRETMAADLPLAVDHFRYFAGCIR 135

Query: 136 AQEGSISQIDDDTVAYHFHEPLGVVGQIIPWNFPLLMACWKLAPAIAAGNCVVLKPAEQT 195
           AQEG IS+IDD+TVAYHFHEP GVVGQIIPWNFPLLMA WKLAPA+AAG C+V+KPAEQT
Sbjct: 136 AQEGGISEIDDNTVAYHFHEPAGVVGQIIPWNFPLLMAAWKLAPALAAGCCIVMKPAEQT 195

Query: 196 PAGIMVWANLIGDLLPPGVLNIVNGFGLEAGKPLASSNRIAKIAFTGETTTGRLIMQYAS 255
           PA ++V   LIGDL P GV+NIVNGFG EAG+ LA+S RIAKIAFTG T  G+ I+  A+
Sbjct: 196 PASVLVLIELIGDLFPAGVINIVNGFGKEAGEALATSKRIAKIAFTGSTPVGKRILHAAA 255

Query: 256 ENLIPVTLELGGKSPNIFFADVAREDDDFFDKALEGFTMFALNQGEVCTCPSRVLIQESI 315
           +NLIP T+ELGGKSPNIFFADV   DD F DKALEG  MFALNQGEVCTCPSRVL+QESI
Sbjct: 256 DNLIPSTVELGGKSPNIFFADVLDRDDAFLDKALEGLAMFALNQGEVCTCPSRVLVQESI 315

Query: 316 YDKFMERAVQRVQAIKQGDPRESDTMIGAQASSEQKEKILSYLDIGKKEGAEVLTGGKAA 375
           Y++F+E+A+ RV+ IK G P   +TMIGAQAS +Q +KILSY+DIG+ EGA  L GG+ A
Sbjct: 316 YERFIEKAIARVERIKAGHPLNLETMIGAQASQQQLDKILSYIDIGRDEGARCLVGGERA 375

Query: 376 DLGGELSGGYYIEPTIFRGNNKMRIFQEEIFGPVVSVTTFKDQAEALEIANDTLYGLGAG 435
             G  L  G+Y++PT+  GNN MR+FQEEIFGPV SV TFKD+ +A+E+ANDT YGLGAG
Sbjct: 376 TPGAGLGEGFYVKPTMLFGNNDMRVFQEEIFGPVASVMTFKDEQQAIELANDTYYGLGAG 435

Query: 436 VWSRDANTCYRMGRGIKAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKN 495
           VW+RD    Y+MGR I+AGRVWTNCYH YPAHAAFGGYKQSGIGRETHKM L +YQQTK 
Sbjct: 436 VWTRDGTRAYKMGREIEAGRVWTNCYHLYPAHAAFGGYKQSGIGRETHKMALSNYQQTKC 495

Query: 496 MLVSYSPKKLGFF 508
           +LVSY  + LGFF
Sbjct: 496 LLVSYQAEALGFF 508


Lambda     K      H
   0.319    0.136    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 855
Number of extensions: 29
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 508
Length of database: 508
Length adjustment: 34
Effective length of query: 474
Effective length of database: 474
Effective search space:   224676
Effective search space used:   224676
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources