Alignments for a candidate for fumD in Mesorhizobium ciceri biovar biserrulae WSM1271

GapMind for catabolism of small carbon sources

Alignments for a candidate for fumD in Mesorhizobium ciceri biovar biserrulae WSM1271

Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate WP_013533408.1 MESCI_RS28435 fumarate hydratase

Query= BRENDA::Q141Z6
         (520 letters)



>NCBI__GCF_000185905.1:WP_013533408.1
          Length = 517

 Score =  630 bits (1626), Expect = 0.0
 Identities = 302/503 (60%), Positives = 393/503 (78%), Gaps = 2/503 (0%)

Query: 17  IKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAEGKR 76
           I  +D+ +S+AD+LQYISYYHP DYI++L +AY  EQSPAAK+AI QIL NSRM A G+R
Sbjct: 11  IAGKDITRSVADALQYISYYHPPDYIRSLSQAYVREQSPAAKNAIGQILANSRMAAFGRR 70

Query: 77  PICQDTGIVTVFVKVGMDVRWDGATMGVTDMINEGVRRGYLNPDNVLRASIVSPPEGGRK 136
           P+CQDTG+V VF KVGMDVR + A     D++N+GVR+ YL+PDN LRASIV+ P   R 
Sbjct: 71  PMCQDTGLVVVFAKVGMDVRINSAA-SFADLVNDGVRQAYLDPDNPLRASIVADPLARRM 129

Query: 137 NTKDNTPAVIHYEIVPGNTVDVQVAAKGGGSENKSKFAMLNPSDSIVDWILKTVPTMGAG 196
           NT+DNTPAV+H ++V GN +++ +AAKGGGSENK++F  LNPS S+ DW++ TV T+G+G
Sbjct: 130 NTRDNTPAVVHVDLVQGNRIEITIAAKGGGSENKARFTTLNPSTSVADWVVDTVSTLGSG 189

Query: 197 WCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNALGI 256
           WCPPG++ +GIGG+AEKAM++AKE++ +PID+ ++IARG     E LR+EL+E++NALGI
Sbjct: 190 WCPPGLISVGIGGSAEKAMLLAKEAMNEPIDMVELIARGASSPEEGLRIELYERINALGI 249

Query: 257 GAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGAARLEAPSLDAW 316
           GAQGLGGL TV+DVK+   PTHAASKPVA+IP CAA RH  FTLDGSG   L+ P L  W
Sbjct: 250 GAQGLGGLTTVVDVKVATYPTHAASKPVALIPQCAANRHVKFTLDGSGPISLQPPDLSEW 309

Query: 317 PKVHWQPDTEKS-KRVDLNTLTPEEVAAWTPGQTLLLSGKMLTGRDAAHKRIADMLAKGE 375
           P++          +R++L+TL  EE A+W  G+TLLLSG MLTGRDAAHKRI +++  G+
Sbjct: 310 PEIGTDELRPAGVRRINLDTLAKEETASWRCGETLLLSGTMLTGRDAAHKRIVELIDAGK 369

Query: 376 KLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTEMMLAQTGLISMIGKAERGP 435
            LP D   RVIYYVGPV  V DE VGPAGPTT++RMD FTE +LA+TGL +M+GKAERG 
Sbjct: 370 PLPFDLQGRVIYYVGPVRAVGDEVVGPAGPTTSSRMDDFTEKVLAETGLFAMVGKAERGR 429

Query: 436 VAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAVDS 495
            AI++I ++K  YL+AVGGA YL+SK+I+SA+++AFEDLGMEA+YEF+V+DMPV  AVD 
Sbjct: 430 AAIQSIARYKTPYLIAVGGAGYLISKSIKSARLVAFEDLGMEAVYEFEVKDMPVIAAVDV 489

Query: 496 NGTSVHQTGPKEWQARIGKIPVA 518
            G S+H +GP EW+ R+    +A
Sbjct: 490 EGNSIHDSGPLEWRKRMAADSIA 512


Lambda     K      H
   0.316    0.133    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 816
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 517
Length adjustment: 35
Effective length of query: 485
Effective length of database: 482
Effective search space:   233770
Effective search space used:   233770
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources