Alignments for a candidate for tpi in Desulfobacca acetoxidans DSM 11109

GapMind for catabolism of small carbon sources

Alignments for a candidate for tpi in Desulfobacca acetoxidans DSM 11109

Align Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 (characterized)
to candidate WP_013706917.1 DESAC_RS16645 triose-phosphate isomerase

Query= SwissProt::P00943
         (253 letters)



>NCBI__GCF_000195295.1:WP_013706917.1
          Length = 254

 Score =  239 bits (610), Expect = 4e-68
 Identities = 121/250 (48%), Positives = 165/250 (66%)

Query: 1   MRKPIIAGNWKMHKTLAEAVQFVEDVKGHVPPADEVISVVCAPFLFLDRLVQAADGTDLK 60
           MR+P+IAGNWKMH TLAE+      V+  +        V+  PF  L  + Q   G+ ++
Sbjct: 4   MRRPLIAGNWKMHLTLAESAALARTVRTDLKETAVAEVVLAPPFTALSVVAQELAGSPVQ 63

Query: 61  IGAQTMHFADQGAYTGEVSPVMLKDLGVTYVILGHSERRQMFAETDETVNKKVLAAFTRG 120
           + AQ + +  QGAYTG +SP ML+D+G  YVI+GHSERRQ F ETD TVN+K+ A  T G
Sbjct: 64  LAAQDVFWELQGAYTGAISPRMLQDVGCRYVIIGHSERRQYFGETDATVNRKIKAVLTVG 123

Query: 121 LIPIICCGESLEEREAGQTNAVVASQVEKALAGLTPEQVKQAVIAYEPIWAIGTGKSSTP 180
           L P++C GE+L +REA +T AV+ SQ+   LA L PE++ + VIAYEP+WAIGTG+++TP
Sbjct: 124 LQPMMCIGETLAQREAEETLAVITSQLTGGLADLLPEEINRLVIAYEPVWAIGTGRTATP 183

Query: 181 EDANSVCGHIRSVVSRLFGPEAAEAIRIQYGGSVKPDNIRDFLAQQQIDGPLVGGASLEP 240
           E A  V   IR ++++   P+ A  +R+ YGGSV PDNI+       IDG LVGGASL+ 
Sbjct: 184 EQAQEVHSFIRQLLAKQVRPDIAYGLRLLYGGSVTPDNIKVLAQAPDIDGALVGGASLKA 243

Query: 241 ASFLQLVEAG 250
            SFL++   G
Sbjct: 244 DSFLKIAALG 253


Lambda     K      H
   0.318    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 186
Number of extensions: 5
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 253
Length of database: 254
Length adjustment: 24
Effective length of query: 229
Effective length of database: 230
Effective search space:    52670
Effective search space used:    52670
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate WP_013706917.1 DESAC_RS16645 (triose-phosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00419.hmm
# target sequence database:        /tmp/gapView.578238.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    3.2e-68  215.9   0.0    3.6e-68  215.8   0.0    1.0  1  NCBI__GCF_000195295.1:WP_013706917.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000195295.1:WP_013706917.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  215.8   0.0   3.6e-68   3.6e-68       1     228 []       8     244 ..       8     244 .. 0.97

  Alignments for each domain:
  == domain 1  score: 215.8 bits;  conditional E-value: 3.6e-68
                             TIGR00419   1 lviinfKlnesvgkvelevaklaeevaseagvevavappfvdldvvkdeve.seiqvaAqnvdavksGaftGe 72 
                                           l+ +n+K++ ++ + + +  +++ ++ ++a+ ev++appf  l+vv++e+  s +q+aAq+v    +Ga+tG 
  NCBI__GCF_000195295.1:WP_013706917.1   8 LIAGNWKMHLTLAESAALARTVRTDLKETAVAEVVLAPPFTALSVVAQELAgSPVQLAAQDVFWELQGAYTGA 80 
                                           689***********************************************999******************** PP

                             TIGR00419  73 isAemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleereaartinnvatt.aaa 144
                                           is  ml+d+G+++v+igHsErR ++ e+d  ++ k+  +   gl++++C+getl++rea +t+ +++ + ++ 
  NCBI__GCF_000195295.1:WP_013706917.1  81 ISPRMLQDVGCRYVIIGHSERRQYFGETDATVNRKIKAVLTVGLQPMMCIGETLAQREAEETLAVITSQlTGG 153
                                           ***************************************************************9998773566 PP

                             TIGR00419 145 aA......lepdvvAvEPveliGtGkpvskAeaevveksvrdhlkk.vskevaesvrvlyGasvtaaedaela 210
                                            A      ++  v+A+EPv++iGtG+++++ +a++v++++r+ l+k v +++a   r+lyG+svt  + + la
  NCBI__GCF_000195295.1:WP_013706917.1 154 LAdllpeeINRLVIAYEPVWAIGTGRTATPEQAQEVHSFIRQLLAKqVRPDIAYGLRLLYGGSVTPDNIKVLA 226
                                           778999999999********************************999************************** PP

                             TIGR00419 211 aqldvdGvLlasavlkae 228
                                             +d+dG+L+++a+lka+
  NCBI__GCF_000195295.1:WP_013706917.1 227 QAPDIDGALVGGASLKAD 244
                                           ****************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (254 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 22.31
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources