Align MsiK protein, component of The cellobiose/cellotriose (and possibly higher cellooligosaccharides), CebEFGMsiK [MsiK functions to energize several ABC transporters including those for maltose/maltotriose and trehalose] (characterized)
to candidate WP_013723230.1 ALIDE2_RS22605 putative 2-aminoethylphosphonate ABC transporter ATP-binding protein
Query= TCDB::P96483 (377 letters) >NCBI__GCF_000204645.1:WP_013723230.1 Length = 377 Score = 219 bits (559), Expect = 7e-62 Identities = 136/355 (38%), Positives = 206/355 (58%), Gaps = 21/355 (5%) Query: 20 AVDQLDIAIEDGEFLVLVGPSGCGKSTSLRMLAGLEDVNGGAIRIGDRDVTHLPPKDRDI 79 A+ +D+ + GE L +GPSGCGK+T LR++AGLE G+I RD++ LP +RD Sbjct: 34 ALRDIDLTVRQGEMLCFLGPSGCGKTTLLRIIAGLETQTSGSIVQSGRDISWLPASERDY 93 Query: 80 AMVFQNYALYPHMTVADNMGFALKIAGVPKAEIRQKVEEAAKILDLTQYLDRKPKALSGG 139 +VFQ+YAL+P++T+A+N+ + L + KAEI+ +V E + L + P LSGG Sbjct: 94 GIVFQSYALFPNLTIAENVAYGLVNGKMRKAEIQARVAELLAMAGLPTAGGKYPSQLSGG 153 Query: 140 QRQRVAMGRAIVREPQVFLMDEPLSNLDAKLRVSTRTQIASLQRRLGITTVYVTHDQVEA 199 Q+QRVA+ RA+ P + L+DEPLS LDA +RV R +I LQ+++GITT+ VTHDQ EA Sbjct: 154 QQQRVALARALATNPGLLLLDEPLSALDATVRVRLRAEIRRLQKQVGITTIMVTHDQEEA 213 Query: 200 MTMGDRVAVLKDGLLQQVDSPRNMYDKPANLFVAGFIGSPAMNLVEVPITDGGVKFGNSV 259 ++M DR+ V+ G+++QV +P +Y++PA+ FVA F+G +N++ GG +F Sbjct: 214 LSMSDRIVVMNHGVIEQVGTPMEIYERPASPFVANFVGK--VNVLRGQAL-GGKRFRVGK 270 Query: 260 VPVNREALSAADKGDRTVTVGVRPEHFDVVELGGAVAASLSKDSADAPAGLAVSVNVVEE 319 + + EA + + V++ +RPE VA L A P L V VN VE Sbjct: 271 MEIECEASEGSFRLGEDVSLYLRPE--------DRVAEHL---EAGTPYRLGVLVNKVEF 319 Query: 320 LGADGYVYGTAE-VGGEVKDLVVRVNGRQVPE----KGSTLHVVPRPGETHVFST 369 LG +AE +GG+ L +N Q+ + +G + + R VFST Sbjct: 320 LGGLCIAEVSAEALGGQSLGLHFSLN--QMHDLGICEGRRIDIALRASRIRVFST 372 Lambda K H 0.317 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 339 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 377 Length of database: 377 Length adjustment: 30 Effective length of query: 347 Effective length of database: 347 Effective search space: 120409 Effective search space used: 120409 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory