Alignments for a candidate for ectP in Thiomicrospira cyclica ALM1

GapMind for catabolism of small carbon sources

Alignments for a candidate for ectP in Thiomicrospira cyclica ALM1

Align Glycine betaine/proline/choline/ectoine transporter VP1456 (characterized)
to candidate WP_013834731.1 THICY_RS00890 choline BCCT transporter BetT

Query= SwissProt::Q87PP5
         (562 letters)



>NCBI__GCF_000214825.1:WP_013834731.1
          Length = 653

 Score =  412 bits (1059), Expect = e-119
 Identities = 212/506 (41%), Positives = 324/506 (64%), Gaps = 13/506 (2%)

Query: 60  KWGFDIHNPVFGISAGLVVFCLISLLLVEPVTARDALNGIKNGIIEQFDAFFMWSTNFFL 119
           K  F I+ PVF IS+ ++   L++  ++    A    +   + I  Q   F++ +   FL
Sbjct: 5   KTAFQINPPVF-ISSAILTLALVAFTIIFNELAASLFSWTLSWISTQLGWFYVLAVAGFL 63

Query: 120 LFAVGLLFSPLGKIRLGGKEATPDHSTVSWLSMLFAAGMGIGLLFWSVAEPTAYFTDWWG 179
           +F   +  S  G ++LG   + P+ + +SW +MLF+AGMGIGL+F+ VAEP  ++     
Sbjct: 64  VFIAFISLSRFGNVKLGSDHSKPEFTNLSWFAMLFSAGMGIGLMFFGVAEPVMHYV---- 119

Query: 180 TPLNAEAYSADAKSLAMGATMFHWGVHGWSIYALVALALAFFAFNKGLPLSLRAAFYPIF 239
           +P   +  +A+A   AM  T FHWGVH W+IYA+VA++LA+FAF   +P+++R++ YP+ 
Sbjct: 120 SPPVGDPATAEAARQAMRITFFHWGVHAWAIYAIVAMSLAYFAFRHDMPMTIRSSLYPLI 179

Query: 240 GDRAWGWLGHVIDILAVLSTLFGLATSLGLGAQQATSGINHVFGLNGGIGTQMVVIAFVT 299
           GDR  G +GH +D  AV+ TLFG+ATSLG G  Q  +G+N++F +  GI TQ+++IA +T
Sbjct: 180 GDRIHGPIGHAVDTFAVIGTLFGVATSLGFGVMQINAGLNYLFDIPVGITTQVILIAVIT 239

Query: 300 FIAVLSVVRGIDGGVKLLSNVNMIVAFALLIFITFITFDTA--MGSLVDTTMAYIQNIIP 357
            IA +SV  G+ GG++ LS +NMI+A ALL F+  IT  T   + +LV  T  YI  ++ 
Sbjct: 240 GIATISVALGLAGGIRRLSILNMILAAALLAFV-LITGPTVFQLQTLVQNTGMYISQLLD 298

Query: 358 LS-NPHGREDETWMHGWTVFYWAWWVSWSPFVGMFIARVSKGRTVREFLFAVIVIPTLVT 416
           ++ N +  E   W+ GWT+FYW WW++W+PFVGMFIA VSKGRT+REF+  V+++P   T
Sbjct: 299 MTFNLYAYEPTDWIGGWTLFYWGWWIAWAPFVGMFIAMVSKGRTIREFVLGVLLVPVGFT 358

Query: 417 LVWMSVFGGIALDQV-VNKVGELGANGLTDISLTLFHVYDVLPYSSVISILSIVLILVFF 475
            +WM+ FGG AL  + V  + EL      D S+ LF   + LP SS+ +I++ +LI+ FF
Sbjct: 359 FMWMTFFGGTALHMIMVQGITELADAVAADTSVALFQFLEHLPLSSITAIIATLLIITFF 418

Query: 476 ITSSDSGSLVIDSITAGGKIDAPVPQRIFWACIEGSIAAVMLWVGGKEALQALQSGVVAT 535
           +TS+DSGSLV+D +T+GG   +   QRIFWA I+G +AA +L  GG   L ALQ+  +A+
Sbjct: 419 VTSADSGSLVVDRLTSGGTGVSHTWQRIFWAVIQGILAAGLLLAGG---LGALQTATIAS 475

Query: 536 GLPFTFVLLLMCVSLVKGLRTELSAY 561
             PF  +++LM   L + LR +++ +
Sbjct: 476 AFPFVIIMILMAWGLARSLRLDVAKH 501


Lambda     K      H
   0.327    0.141    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1035
Number of extensions: 64
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 562
Length of database: 653
Length adjustment: 37
Effective length of query: 525
Effective length of database: 616
Effective search space:   323400
Effective search space used:   323400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources