Alignments for a candidate for betS in Nocardiopsis lucentensis DSM 44048

GapMind for catabolism of small carbon sources

Alignments for a candidate for betS in Nocardiopsis lucentensis DSM 44048

Align Glycine betaine/proline betaine transporter BetS (characterized)
to candidate WP_040690304.1 D471_RS35115 BCCT family transporter

Query= SwissProt::G3XCN6
         (706 letters)



>NCBI__GCF_000341125.1:WP_040690304.1
          Length = 578

 Score =  445 bits (1145), Expect = e-129
 Identities = 231/480 (48%), Positives = 321/480 (66%), Gaps = 8/480 (1%)

Query: 57  VFVGSVAVIALFVGIGVIAPKRAESIFSGMQTAILSGFGWLYLLSVAVFLFSMLFLAFSR 116
           VF  SV ++ +FV    +      +  + +Q  ++  FGW Y+L V  F+   + +   R
Sbjct: 7   VFWPSVILVTIFVAFTAVFTDVVSTAITTLQDTVIGAFGWYYILIVCGFVVFSIRVGLGR 66

Query: 117 YGELKLGPDDSEPEFRYLSWIAMLFAAGMGIGLMYFAVGEPMTHFASP-PEAEPLTIAAQ 175
           +G++KLGPDD EPEF+  +W +MLFAAGMGIGL+++ V EP+ HFASP P  E  +    
Sbjct: 67  FGDIKLGPDDEEPEFKLGTWFSMLFAAGMGIGLVFWGVAEPLNHFASPKPGVEGTSQELA 126

Query: 176 REAMSVTFFHWGVHAWAIYSVVGLSLAYFGYRYNLPLTVRSGLYPLL-KEGIHGPIGHVV 234
           ++++  TF HWG+H WAIY VVGL++AY  +R   P+++R  L PLL +  ++G +G ++
Sbjct: 127 QQSLVQTFLHWGLHPWAIYVVVGLAIAYAIHRKKRPVSIRWALEPLLGRRRVNGWLGDLI 186

Query: 235 DIFAICGTMFGLATSLGFGILQINSGLNYLLGIPQSI--YVQLLLVTVVTAIATISVVTG 292
           D+ A+ GT+FG+ATSLG G+LQI SGL+ +LGI      +  ++L+  +TA+A  SV TG
Sbjct: 187 DVVAVIGTLFGVATSLGLGVLQIASGLD-VLGIVSDPGNWTNIVLIGGITALAIFSVTTG 245

Query: 293 VEKGVRILSETNLFLAVLLMLFVLVVGPTGTLMRDFVQNIGLYLDSLVLRTFNIYAYEPR 352
           V++GV+ LS+ N+ LAV+LML VLV GPT  L+R+FVQ+IGLY  +L+  +F+  A E +
Sbjct: 246 VKRGVKWLSQINMGLAVVLMLIVLVTGPTLFLLREFVQSIGLYFQNLLRLSFDTTALEGQ 305

Query: 353 ---PWIDSWTLFYWAWWISWSPFVGMFIARISRGRTVREFVTAVLFVPAMFTFLWMTVFG 409
               W   WT FYW WW+SW+PFVG+FIARISRGRTVREFVT VL VP   TFLW+TVFG
Sbjct: 306 GGAQWQGWWTTFYWGWWMSWAPFVGVFIARISRGRTVREFVTGVLLVPTAVTFLWLTVFG 365

Query: 410 NTAIYVDTTIANGELARDVKADLSVALFQFFEYLPWPAVTSTLAVLLVSIFFVTSSDSGS 469
            +A+Y +   + G +A D   +   ALF     LP   V    AV+L+ +FFVTSSDSGS
Sbjct: 366 GSALYRELFGSGGGVAADGSVNTEGALFGLLGELPGGTVLVAGAVILIVLFFVTSSDSGS 425

Query: 470 LVIDTIASGGETATPALQRIFWCSLSGIVAAVLLSTGGLTALQSATISTALPFSLVMLIL 529
           LV+D +ASGG   TP   R+FW +  G+VA  LL  GGL+ALQ   I  ALPFS+VML++
Sbjct: 426 LVVDMLASGGSHETPVWSRVFWAAAEGLVAITLLLAGGLSALQVGAILIALPFSVVMLLM 485


Lambda     K      H
   0.326    0.139    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1101
Number of extensions: 58
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 706
Length of database: 578
Length adjustment: 38
Effective length of query: 668
Effective length of database: 540
Effective search space:   360720
Effective search space used:   360720
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources