GapMind for catabolism of small carbon sources

 

Protein WP_022670152.1 in Hippea alviniae EP5-r

Annotation: NCBI__GCF_000420385.1:WP_022670152.1

Length: 251 amino acids

Source: GCF_000420385.1 in NCBI

Candidate for 22 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine catabolism artP lo Arginine transport ATP-binding protein ArtM (characterized) 38% 98% 148.7 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-asparagine catabolism bztD lo BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) 33% 93% 140.6 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-aspartate catabolism bztD lo BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) 33% 93% 140.6 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-glutamate catabolism gltL lo BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) 33% 93% 140.6 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-histidine catabolism hisP lo Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 36% 97% 140.2 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-lysine catabolism hisP lo Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 36% 97% 140.2 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-asparagine catabolism aatP lo ABC transporter for L-asparagine and L-glutamate, ATPase component (characterized) 37% 92% 139.4 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-aspartate catabolism aatP lo ABC transporter for L-asparagine and L-glutamate, ATPase component (characterized) 37% 92% 139.4 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-asparagine catabolism aapP lo AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 32% 93% 136.7 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-aspartate catabolism aapP lo AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 32% 93% 136.7 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-glutamate catabolism aapP lo AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 32% 93% 136.7 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-histidine catabolism aapP lo AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 32% 93% 136.7 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-leucine catabolism aapP lo AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 32% 93% 136.7 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-proline catabolism aapP lo AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 32% 93% 136.7 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-asparagine catabolism peb1C lo PEB1C, component of Uptake system for glutamate and aspartate (characterized) 36% 98% 135.2 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-aspartate catabolism peb1C lo PEB1C, component of Uptake system for glutamate and aspartate (characterized) 36% 98% 135.2 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-histidine catabolism bgtA lo BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) 34% 95% 133.7 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-proline catabolism opuBA lo BilEA aka OpuBA protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) (characterized) 35% 69% 132.1 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
D-alanine catabolism Pf6N2E2_5405 lo ABC transporter for D-Alanine, ATPase component (characterized) 31% 93% 131.3 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-citrulline catabolism AO353_03040 lo ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized) 34% 98% 126.3 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-asparagine catabolism bgtA lo ATPase (characterized, see rationale) 31% 92% 123.2 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4
L-aspartate catabolism bgtA lo ATPase (characterized, see rationale) 31% 92% 123.2 Phosphate import ATP-binding protein PstB, component of The phosphate transporter, PstABCS 60% 305.4

Sequence Analysis Tools

View WP_022670152.1 at NCBI

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Find functional residues: SitesBLAST

Search for conserved domains

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Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

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Sequence

MNQVLLSVKNLNFFYGKTQILHNINMDIYKNRITALIGPSGCGKTTFLRCFNRMHDLYKG
NRYEGEILYKGENILKVKDLIELRSKIGMVFQKPTPFPMSIFDNVAYGLKLKGIKDKNII
RERVEKALIEAALWDEVKDKLYSSALALSGGQQQRMVIARALAVEPDIILFDEPTSALDP
IATSKIEELLFNLKKITTILIVTHNMQQAARVSDFTAFLFKGHLIEFERTEKLFTNPKEE
LTEKYITGRFG

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory