Alignments for a candidate for gabD in Hippea alviniae EP5-r

GapMind for catabolism of small carbon sources

Alignments for a candidate for gabD in Hippea alviniae EP5-r

Align Succinate-semialdehyde dehydrogenase, mitochondrial; At-SSADH1; Aldehyde dehydrogenase family 5 member F1; NAD(+)-dependent succinic semialdehyde dehydrogenase; Protein ENLARGED FIL EXPRESSING DOMAIN 1; EC 1.2.1.24 (characterized)
to candidate WP_022669887.1 G415_RS0101870 aldehyde dehydrogenase family protein

Query= SwissProt::Q9SAK4
         (528 letters)



>NCBI__GCF_000420385.1:WP_022669887.1
          Length = 474

 Score =  344 bits (883), Expect = 4e-99
 Identities = 182/471 (38%), Positives = 298/471 (63%), Gaps = 12/471 (2%)

Query: 57  IGGKWLDSYDNKTIKVNNPATGEIIADVACMGTKETNDAIASSYEAFTSWSRLTAGERSK 116
           IGG+W+ +  NKTI V +  +GE+IA V     K  + A+ ++ EAF  + ++ A +RS+
Sbjct: 8   IGGEWVST--NKTIDVIDKYSGEVIAKVPKADKKLVDKAVDAAAEAFEEFKKMPAYQRSE 65

Query: 117 VLRRWYDLLIAHKEELGQLITLEQGKPLKEAIGEVAYGASFIEYYAEEAKRVYGDIIPPN 176
           +L +  DL+    +E+ + I  E GK  K ++GEV  G    ++ +EEAKR++G+ +P +
Sbjct: 66  ILEKAADLIKERSDEIAETICREAGKAWKYSVGEVNRGFETFKFASEEAKRIHGETVPMD 125

Query: 177 LS----DRRLLVLKQPVGVVGAITPWNFPLAMITRKVGPALASGCTVVVKPSELTPLTAL 232
            S     R    ++ P+GV+GAITP+NFPL ++  KV PA+ASG T+V+KP+  TP+TAL
Sbjct: 126 ASAGGVGRVGYYIRVPMGVLGAITPFNFPLNLVAHKVAPAIASGNTIVLKPASSTPITAL 185

Query: 233 AAAELALQAGVPPGALNVVMGNAPEIGDALLTSPQVRKITFTGSTAVGKKLMAAAAPTVK 292
             AE+  +AG+P G  NVV+G   E+G++L+T+ +VRKITFTGS  VG +L   A   +K
Sbjct: 186 ILAEILEEAGLPKGVFNVVIGPGGEVGESLVTNDKVRKITFTGSAKVGHRLTQIAG--IK 243

Query: 293 KVSLELGGNAPSIVFDDADLDVAVKGTLAAKFRNSGQTCVCANRVLVQDGIYDKFAEAFS 352
           +++LELG N+ +I+  DAD+D A+   + + F NSGQ C+   R+ V   I D+F E F+
Sbjct: 244 RITLELGNNSATIIEKDADIDKAIPRCVDSAFANSGQVCISLQRIYVHKDIADEFTEKFA 303

Query: 353 EAVQKLEVGDGFRDGTTQGPLINDAAVQKVETFVQDAVSKGAKIIIGGKRHSLGMTFYEP 412
           EA +KL+VG+     T  GP+I++   ++ E ++++AV+ GA++++GGKR        +P
Sbjct: 304 EATKKLKVGNPVEKDTDLGPMIDENEAKRAEEWIKEAVNMGAELVVGGKREG---RVLQP 360

Query: 413 TVIRDVSDNMIMSKEEIFGPVAPLIRFKTEEDAIRIANDTIAGLAAYIFTNSVQRSWRVF 472
           TV+R+ + +M +   E+F P+  ++ + T EDAI+  ND+  GL A I+TN +++     
Sbjct: 361 TVLRNTTKDMRVMCMEVFAPIVSIVEYDTFEDAIQHVNDSDYGLQAGIYTNDIRKIHYAI 420

Query: 473 EALEYGLVGVNE-GLISTEVAPFGGVKQSGLGREGSKYGMDEYLEIKYVCL 522
           + L+ G V +N+  +   +  P+GG K SG+GREG ++ ++E   IK V +
Sbjct: 421 DNLDVGGVMINDTSIFRVDHMPYGGNKMSGIGREGVRFAIEEMTNIKMVMI 471


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 605
Number of extensions: 32
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 528
Length of database: 474
Length adjustment: 34
Effective length of query: 494
Effective length of database: 440
Effective search space:   217360
Effective search space used:   217360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources