Alignments for a candidate for drdehyd-beta in Thermithiobacillus tepidarius DSM 3134

GapMind for catabolism of small carbon sources

Alignments for a candidate for drdehyd-beta in Thermithiobacillus tepidarius DSM 3134

Align 2-deoxy-D-ribose dehydrogenase β subunit (characterized)
to candidate WP_038019894.1 G579_RS17460 molybdopterin-dependent oxidoreductase

Query= metacyc::MONOMER-20833
         (739 letters)



>NCBI__GCF_000423825.1:WP_038019894.1
          Length = 822

 Score =  393 bits (1010), Expect = e-113
 Identities = 260/691 (37%), Positives = 358/691 (51%), Gaps = 45/691 (6%)

Query: 52  QVPAFLEIRPDGTVRLLSPFMEGGQGTHTAMAQIVGEELDADPATFIVEAAPPGEAYVVM 111
           Q   ++ I   GTV +L    E GQG  T +A +V EEL+ D +    E AP G AY+  
Sbjct: 167 QFDPWIRIDEHGTVTILIDRAEMGQGVLTGLAMLVAEELEVDLSAVRTEFAPAGPAYINP 226

Query: 112 ENGLRITGGSMSVRMSYPTMRRLGALARAMLLQAGAKQWGVPVGELTTQPGRVVHAASGR 171
             G + TGGS +VR ++  +RR GA AR  L+ A A+ W V         G V+H +SGR
Sbjct: 227 LLGKQSTGGSTAVRAAWEPLRRAGAQARERLITAAARIWSVEAASCRAAHGAVLHPSSGR 286

Query: 172 SLGYGELASSALDMPVPDPASITLRDPSQFRWIGKPVKRLDAYDKSTGKALYSIDLKVDN 231
            L YG LA+ A  +P P    + L+ P+ FR IG+P   LDA  K TG+A++ +D++V  
Sbjct: 287 RLDYGALAARAAALPAP--TQVVLKPPTAFRLIGQPTPMLDAPLKITGRAVFGVDVQVPG 344

Query: 232 MLHAAVQHAPRLGMTVGSLRNQSQVEGMKGVHSVHVLPGAVAVVAERWWHAKRAVEAIQV 291
           M  A V   P  G    S+  ++  E + GV  +  +   +AV+A+ +W AK+  EA+ V
Sbjct: 345 MRVAVVLRCPSFGGRAVSVDVRA-AEAVPGVQRIVPIASGIAVIADDFWAAKQGREALAV 403

Query: 292 DWQE---AAADSA-LRVMPADFSSDKHREFLAAQQGPTRDDENEGDVAGALKGAKTQVEA 347
            W+    AA DSA +R   A  + D           P RDD   G +  A   A  ++EA
Sbjct: 404 QWEAGPLAALDSAAIRTALAAAARDPGT--------PVRDD---GQLDAAFAQAGRRLEA 452

Query: 348 TYHNQYLNHAQLEPPSALARFNPDGTLEIWLPNQAPDMFRADIAKRTGLDPAQITLHSPL 407
            Y   YL HA +EP +  A    DG  ++W+P QA    +  +A+ TGL PA I +HS  
Sbjct: 453 VYETPYLAHAAMEPMNCTAHVRADGC-DVWVPTQAQGGAQETVAELTGLSPAAIRVHSTF 511

Query: 408 LGGFFGRHFLYDSANPYPQAIALAKAVSRPIKLIWSREEEFLRDVLRPLAVVKFRAALDD 467
           LGG FGR    D      +A+ L++AV  P++++W+RE++   D  RP       AALD 
Sbjct: 512 LGGGFGRRLQQDFV---AEAVQLSRAVDAPMQVVWTREDDLRHDHYRPANHNYLAAALDA 568

Query: 468 KGLPVAIEAVSATEGPTEALAGKQGDKIDPTAVEGLSGKSYAIPNKRIAQIYVKGAPMLG 527
            G P+A   +    GP  +L G                  YAIP+ R+           G
Sbjct: 569 HGRPLAW--LHRVTGPAASLDGAHFP--------------YAIPHVRVEHTEADPGVPTG 612

Query: 528 YWRSVGNSLNDFFYESFLDELADKGGHDPYELRLHLLRDNPRLTTLLQAAGELSGGWKRG 587
            WRSV +S + F  E F+DELA   G DPY  RL LL   PR   +L+ A E +G  +  
Sbjct: 613 AWRSVAHSQSAFAIEGFVDELATAAGADPYAFRLDLLHHAPRHQRVLELAAERAGWGRAL 672

Query: 588 PFTAEDGTRRARGVAMASPFGSHTAAIAEVSIE-NGKVKVHDIWQAIDPGSIVNPAIVEA 646
           P      +   RG A+   FGS  A +AEVS+  +G+V+VH I  A+D G  VNP  V A
Sbjct: 673 P------SGHGRGTAVHQSFGSWVAQVAEVSVAGDGRVRVHRIVCAVDCGLAVNPNAVAA 726

Query: 647 QVNGAVALGLSQTLLEEAVYVDGKPRARNYDLYPILAPAQMARVHVRVVESGEKMGGIGE 706
           Q+ GAVA GLS  L  E     G+    N+D YPIL   +M  V V ++ S E  GG+GE
Sbjct: 727 QMEGAVAFGLSAALKGEITLTHGRVLQGNFDDYPILRMDEMPLVEVHILPSREPPGGVGE 786

Query: 707 PPLPAVAPAVANAVAQLTGQRVRSLPLSRHT 737
           P +P VAPAVANAV   TG+R+R LP+   T
Sbjct: 787 PGVPPVAPAVANAVFAATGRRIRRLPIRAET 817


Lambda     K      H
   0.317    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1588
Number of extensions: 78
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 739
Length of database: 822
Length adjustment: 41
Effective length of query: 698
Effective length of database: 781
Effective search space:   545138
Effective search space used:   545138
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources