Alignments for a candidate for bamH in Thermithiobacillus tepidarius DSM 3134

GapMind for catabolism of small carbon sources

Alignments for a candidate for bamH in Thermithiobacillus tepidarius DSM 3134

Align Benzoyl-CoA reductase electron transfer protein, putative (characterized, see rationale)
to candidate WP_028989730.1 G579_RS0107730 NADH-quinone oxidoreductase subunit NuoF

Query= uniprot:Q39TW5
         (635 letters)



>NCBI__GCF_000423825.1:WP_028989730.1
          Length = 422

 Score =  364 bits (935), Expect = e-105
 Identities = 187/413 (45%), Positives = 264/413 (63%), Gaps = 5/413 (1%)

Query: 142 ENLRLDSK-SMDDYLAIGGYSALSKVL-FQMTPEDVMGEIKKSNLRGRGGGGFPAWRKWE 199
           +NL L    ++  Y + GGY  L KVL   ++PED++ E+KKS LRGRGG GFP   KW 
Sbjct: 8   QNLHLKEPWTLSTYASTGGYDMLRKVLQTPVSPEDIINEVKKSALRGRGGAGFPTGLKWS 67

Query: 200 ESRNAPDPIKYVIVNADEGDPGAFMDRALIEGNPHSILEGLIIGAYAVGAHEGFIYVRQE 259
                    KY++ NADEG+PG   DR ++  +PH ++EG+ I AYA+GA+ G+IY+R E
Sbjct: 68  FMPKGVGHPKYLLCNADEGEPGTCKDRDIMRYDPHRLIEGMAIAAYAIGANVGYIYIRGE 127

Query: 260 YPLAVENINLAIRQASERGFVGKDILGSGFDFTVKVHMGAGAFVCGESSALMTALEGRAG 319
           +    E +  AI +A   GF+GK+ILGS   F +  H GAGA++CGE +AL+ +LEG+ G
Sbjct: 128 FYEPWERVQNAIDEAYAAGFLGKNILGSDLSFDLYTHRGAGAYICGEETALIESLEGKKG 187

Query: 320 EPRPKYIHTAVKGVWDHPSVLNNVETWANVTQIITKGADWFTSYGTAGSTGTKIFSLVGK 379
           +PR K    A  G++  P+ +NNVET A+V  II  G +WF + G   + GTK+FS+ G 
Sbjct: 188 QPRFKPPFPASYGLYGRPTTVNNVETLASVPDIIRHGGEWFLNLGKPNNGGTKLFSVTGH 247

Query: 380 ITNTGLVEVPMGVTLRDIITKVGGGIPGGKKFKAVQTGGPSGGCIP-EAMLDLPVDFDEL 438
           +   G  EVPMG   R+++  + GG+  G+K KAV  GG S   +P E M+++ +D+D +
Sbjct: 248 VNKPGNFEVPMGTPFRELLA-MAGGVRDGRKLKAVIPGGSSTPMVPGEVMMEVNMDYDSI 306

Query: 439 TKAGSMMGSGGMIVMDEDTCMVDIARYFIDFLKDESCGKCTPCREGIRQMLAVLTRITVG 498
           +KAGSM+G+G +IVMDE TC+V  A     F  DESCG+CTPCREG   +  +L RI  G
Sbjct: 307 SKAGSMLGAGSVIVMDETTCIVQAAERMARFYYDESCGQCTPCREGTGWLYRILRRIESG 366

Query: 499 KGKEGDIELLEELAES-TGAALCALGKSAPNPVLSTIRYFRDEYEAHIREKKC 550
           +G E D+ELL  L  +  G  +CALG +A  PV S+I++FR EYE HI+  +C
Sbjct: 367 QGTEADLELLLNLGNNIEGRTICALGDAAVAPVASSIKHFRAEYEHHIKHGRC 419


Lambda     K      H
   0.319    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 789
Number of extensions: 44
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 635
Length of database: 422
Length adjustment: 35
Effective length of query: 600
Effective length of database: 387
Effective search space:   232200
Effective search space used:   232200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources