Alignments for a candidate for gguA in Maridesulfovibrio zosterae DSM 11974

GapMind for catabolism of small carbon sources

Alignments for a candidate for gguA in Maridesulfovibrio zosterae DSM 11974

Align GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized)
to candidate WP_027722484.1 H589_RS0113340 ABC transporter ATP-binding protein

Query= TCDB::O05176
         (512 letters)



>NCBI__GCF_000425265.1:WP_027722484.1
          Length = 513

 Score =  238 bits (606), Expect = 5e-67
 Identities = 157/485 (32%), Positives = 254/485 (52%), Gaps = 16/485 (3%)

Query: 5   ILEMRNITKTFPGVKALENVNLKVKEGEIHALVGENGAGKSTLMKVLSGVYPAGTYEGEI 64
           ++ ++ ITK F  V A  N++L +  G I AL+GENGAGKSTLM +L+G +     EG I
Sbjct: 24  LISLKGITKRFGKVVANNNISLDLYPGRIKALLGENGAGKSTLMSMLAGRFRPD--EGFI 81

Query: 65  HYEGAVRNFRAINDSEDIGIIIIHQELALVPLLSIAENIFLGNEVASNGVISWQQTFNRT 124
             +G   +F    D+   G+ +++Q   LV  +++AEN+ LG E   +  +S ++   R 
Sbjct: 82  EVDGQRVDFSNSKDAIKAGVGMVYQHFMLVDTMTVAENVLLGQE--GSFFVSPKEMNERV 139

Query: 125 RELLKKVGLKESPETLITDIGVGKQQLVEIAKALSKSVKLLILDEPTASLNESDSEALLN 184
           R++ +   L+  P   + D+ +G++Q VEI K L +  ++LI DEPTA L   ++  L  
Sbjct: 140 RQIAEDYELEIDPSARVQDLSMGEKQRVEIIKLLYRESRVLIFDEPTAVLTPREAFRLFE 199

Query: 185 LLMEFRNQGMTSIIITHKLNEVRKVADQITVLRDGMTVKTLDCHQEEISEDVIIRNMVGR 244
            L     QG + + I+HKL EV  +AD++ +LR G     +  ++     D+  R MVG+
Sbjct: 200 ALWAMTRQGKSVVFISHKLEEVMAIADEVAILRRGEIDAEVPRNKIVSKADLACR-MVGK 258

Query: 245 DLEDRYPPRDVPIGETILEVKNWNAYHQQHRDRQVLHDINVTVRKGEVVGIAGLMGAGRT 304
           ++       DV IG+ +LEVKN             L  IN+ VRKGEVV I G+ G G+ 
Sbjct: 259 EVLLEIHKEDVEIGDKVLEVKNMTGIG--------LRGINLDVRKGEVVAIVGVAGNGQQ 310

Query: 305 EFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRKAIDAGLAYVTEDRKHLGLVLNDNILHN 364
           E    V G     + T  + I  KP          +  ++Y+ EDR  L    N +++ N
Sbjct: 311 ELVEGVCGLRKPPKDT--IFIMNKPWRKFFAEMKWNNSMSYIPEDRLDLATARNLDLVDN 368

Query: 365 TTLANLAGVSKASIIDDIKEMKVASDFRTRLRIRSSGIFQETVNLSGGNQQKVVLSKWLF 424
             L    G +   I+   K  KVA +      +R   I      LSGGN QK+VL++ L+
Sbjct: 369 LLLTTRQGFTSGPILQRDKAAKVAEELVKDYDVRPGRIQALAWQLSGGNLQKMVLARELY 428

Query: 425 SNPDVLILDEPTRGIDVGAKYEIYTIINQLAADGKGVLMISSEMPELLGNCDRIYVMNEG 484
             P +++ ++PT+G+D+ A  E++  + + A    G+L+++ ++ E L   DR+ VM  G
Sbjct: 429 RQPHLIVAEQPTQGLDISATEEVWNKLLE-ARKMAGILLVTGDLGEALQLADRVAVMYCG 487

Query: 485 RIVAE 489
           +I+ E
Sbjct: 488 QIMDE 492



 Score = 73.6 bits (179), Expect = 2e-17
 Identities = 59/219 (26%), Positives = 103/219 (47%), Gaps = 20/219 (9%)

Query: 281 HDINVTVRKGEVVGIAGLMGAGRTEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRKAID 340
           ++I++ +  G +  + G  GAG++     + G+       G + +DG+ VD S  + AI 
Sbjct: 41  NNISLDLYPGRIKALLGENGAGKSTLMSMLAGRFRPDE--GFIEVDGQRVDFSNSKDAIK 98

Query: 341 AGLAYVTEDRKHLGLVLNDNILHNTTLANLAGVSKASIIDDIKEM-----KVASDFRTRL 395
           AG+  V +   H  LV    +  N  L       + S     KEM     ++A D+   L
Sbjct: 99  AGVGMVYQ---HFMLVDTMTVAENVLLGQ-----EGSFFVSPKEMNERVRQIAEDYE--L 148

Query: 396 RIRSSGIFQETVNLSGGNQQKVVLSKWLFSNPDVLILDEPTRGIDVGAKYEIYTIINQLA 455
            I  S   Q+   LS G +Q+V + K L+    VLI DEPT  +     + ++  +  + 
Sbjct: 149 EIDPSARVQD---LSMGEKQRVEIIKLLYRESRVLIFDEPTAVLTPREAFRLFEALWAMT 205

Query: 456 ADGKGVLMISSEMPELLGNCDRIYVMNEGRIVAELPKGE 494
             GK V+ IS ++ E++   D + ++  G I AE+P+ +
Sbjct: 206 RQGKSVVFISHKLEEVMAIADEVAILRRGEIDAEVPRNK 244


Lambda     K      H
   0.316    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 576
Number of extensions: 31
Number of successful extensions: 11
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 513
Length adjustment: 35
Effective length of query: 477
Effective length of database: 478
Effective search space:   228006
Effective search space used:   228006
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources