Alignments for a candidate for pimF in Azohydromonas australica DSM 1124

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Azohydromonas australica DSM 1124

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate WP_028998564.1 H537_RS0115025 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein

Query= metacyc::MONOMER-20678
         (699 letters)



>NCBI__GCF_000430725.1:WP_028998564.1
          Length = 697

 Score =  670 bits (1728), Expect = 0.0
 Identities = 373/689 (54%), Positives = 475/689 (68%), Gaps = 18/689 (2%)

Query: 13  VAIVTVDSPPVNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADITEFGKPP 72
           VA++T+D+PPVN +S A+R  +L  ++ A+AD  V+A+VL    + F AGAD+ EFG P 
Sbjct: 13  VAVITLDNPPVNGISHALRSRVLALLDEALADAQVRAVVLTGGEKLFSAGADVREFGTPK 72

Query: 73  --QPPALNDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEAKLGLPEVKLGL 130
             Q P L  +IAALE+S KP +AAI GT LGGGLE+ LGCHFR+A  +A LGLPEVKLGL
Sbjct: 73  SGQQPNLPALIAALESSSKPVVAAIAGTCLGGGLELTLGCHFRIAKADASLGLPEVKLGL 132

Query: 131 LPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVVE--NLVAGAVAFAKKV 188
           LPGAGGTQRLPRA+G E A+ MI+ G P  AA     GL++ VV   NL   AVAFA++V
Sbjct: 133 LPGAGGTQRLPRAIGLETALNMILSGEPQPAARFEGSGLLDAVVTEGNLAQAAVAFAEQV 192

Query: 189 LAEKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTKKA-RGLEAPFACADAIGAAIDLPF 247
           +AE RP +RLRD   KL  A +D   F        K A R L AP  C DA+ A + LPF
Sbjct: 193 VAEGRPPKRLRD--VKLEQAGSDA--FLQFARTQVKAANRFLPAPLKCVDAVAACVSLPF 248

Query: 248 EEGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPRPVSRVAIIGAGTMG 307
           ++GL+ ERE F+ L+ + +S+A R+ F AER A+K D VP     R V +V IIGAGTMG
Sbjct: 249 DDGLRFERELFLGLMNTAESRALRHVFAAERAASKAD-VPASAPLREVRQVGIIGAGTMG 307

Query: 308 GGIAMSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLPPDAPAKRMALITGLV 367
           GGIAM+FANAG+PV L+E  +  L +G   ++ N+E +  +G L      +R+ALIT  +
Sbjct: 308 GGIAMAFANAGLPVVLLEASQAALDKGFNTIRANYEGSLKKGKLTQAQFEQRLALITPTL 367

Query: 368 GLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSYLSIDEIAATTKRPQD 427
             E+++DADL IEAVFE++ VK++VF  +D   KPGA+LASNTS L +D IA  T+RPQD
Sbjct: 368 SYESLRDADLAIEAVFESLEVKEQVFRTLDEVMKPGAILASNTSALDLDAIARFTRRPQD 427

Query: 428 VLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVVVGVCDGFVGNRMLAA 487
           V+G+HFFSPANVM+L E+VRGA TAP+ L TA+ +AK+I KV VV GVCDGF+GNR+LA 
Sbjct: 428 VIGLHFFSPANVMRLLEVVRGAATAPEVLATAMQLAKRIKKVAVVSGVCDGFIGNRILAR 487

Query: 488 RSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWRSRKDRGIK------S 541
               +  LL  GALPQQVD  +  FG  MG F +GDLAGLDIGW  RK R         S
Sbjct: 488 YGAAANDLLDRGALPQQVDRALEDFGFAMGLFRVGDLAGLDIGWAGRKRRAAANPGQDFS 547

Query: 542 EIADALCEAGRFGQKTGKGYYKYEQGSRAPMPDPEVETLINDTLAKLGLKRRDITDEEIL 601
            +AD +CEAGRFGQKTG G+Y+YE G R P+PDP V  +I    A+ G   R I  +EI+
Sbjct: 548 IVADRVCEAGRFGQKTGAGWYRYEPGRRDPIPDPAVTQIIEQWRAERGHATRAIDAQEIV 607

Query: 602 ERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGPMHYADSVGLKHIAERLS 661
           ER V+ M+NEGARIL E IA R SDIDVV+L GYG+P +RGGPM YAD VGL ++  RL 
Sbjct: 608 ERCVFAMVNEGARILAEGIAQRASDIDVVYLNGYGFPKHRGGPMKYADEVGLPYVVRRLK 667

Query: 662 AY-AKATNDPSL-EPAPLLARLAAEGKTF 688
           A+ A+A  + +L  PAPLL +LAAEG +F
Sbjct: 668 AFAAEAGAEAALWTPAPLLLQLAAEGGSF 696


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1151
Number of extensions: 57
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 697
Length adjustment: 39
Effective length of query: 660
Effective length of database: 658
Effective search space:   434280
Effective search space used:   434280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources