Alignments for a candidate for acs in Derxia gummosa DSM 723

GapMind for catabolism of small carbon sources

Alignments for a candidate for acs in Derxia gummosa DSM 723

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized)
to candidate WP_028312775.1 H566_RS0119635 propionate--CoA ligase

Query= curated2:O93730
         (670 letters)



>NCBI__GCF_000482785.1:WP_028312775.1
          Length = 634

 Score =  499 bits (1286), Expect = e-145
 Identities = 272/637 (42%), Positives = 402/637 (63%), Gaps = 21/637 (3%)

Query: 32  AYFKFHRQTVENLESFWESVAKELEWFKPWDKVLDASNPPFYKWFVGGRLNLSYLAVDRH 91
           AY  FH+++VE+ E FW   +  +EW     +VLD SNPPF +WFVGG  NL + A+DRH
Sbjct: 6   AYEAFHQRSVEDAEGFWREQSALIEWKDAPRQVLDFSNPPFARWFVGGTTNLCHNAIDRH 65

Query: 92  VKTWRKNKLAIEWEGEPVDENGYPTDRRKLTYYDLYREVNRVAYMLKQNFGVKKGDKITL 151
           +K  R ++ AI +    VD+       R  T+ D++REVN+VA   K+  GV KGD++ +
Sbjct: 66  LKD-RADQAAIIFISTEVDQE------RVYTFADVHREVNKVAAAYKE-LGVGKGDRVLI 117

Query: 152 YLPMVPELPITMLAAWRIGAITSVVFSGFSADALAERINDSQSRIVITADGFWRRGRVVR 211
           Y+PM+PE    MLAA RIGA+ SVVF GF++ +LA RI+D+   ++++AD   R G+VV 
Sbjct: 118 YMPMIPEALFAMLAAVRIGAVHSVVFGGFASHSLATRIDDATPALIVSADAGARSGKVVE 177

Query: 212 LKEVVDAALEKATGVESVIVLPRLGLKDVPMTEGRDYWWNKLMQGIPPNAYIEPEPVESE 271
            K ++DAA++ A      +++   GL      EGRD  +  +       A +  E +ES 
Sbjct: 178 YKPLLDAAIDLAAHKPPKVLMVDRGLAKFAPVEGRDVDYATITTKHAA-AEVPVEWLESN 236

Query: 272 HPSFILYTSGTTGKPKGIVHDTGGWAVHVYATMKWVFDIRDDDIFWCTADIGWVTGHSYV 331
            PS+ILYTSGTTGKPKG+  DTGG+AV + A++K+++     + F+ T+DIGWV GHSY+
Sbjct: 237 EPSYILYTSGTTGKPKGVQRDTGGYAVALAASIKYIYQGEPGETFFSTSDIGWVVGHSYI 296

Query: 332 VLGPLLMGATEVIYEGAPDYPQPDRWWSIIERYGVTIFYTSPTAIRMFMRYGEEWPRKHD 391
           V GPLL G   V+YEG P  P    WW I+E+Y V++ +++PTA+R+  +       K+D
Sbjct: 297 VYGPLLAGMATVVYEGTPLRPDAGIWWQIVEKYKVSLMFSAPTAVRVLKKQDPTLLSKYD 356

Query: 392 LSTLRIIHSVGEPINPEAWRWAYRVLGNEKVAFGSTWWMTETGGIVISHAPGLYL-VPMK 450
           LS+LR +   GEP++    +W    +G    A    +W TETG  ++S A G+   + +K
Sbjct: 357 LSSLRALFLAGEPLDEPTAQWISDGIGK---AILDNYWQTETGWPILSVARGVQPGMDIK 413

Query: 451 PGTNGPPLPGFEVDVVDENGNPAPPGVKGYLVIKKPW-PGMLHGIWGDPERYIKTYWSRF 509
            G+ G P  G+ V +++E G  A  G KG + +  P  PG +  IWGD  R++KTY++ F
Sbjct: 414 FGSPGRPTYGYNVKLLNEQGEEAAAGEKGVIAVMPPLPPGCMSTIWGDDARFVKTYFTSF 473

Query: 510 P-GMFYAG-DYAIKDKDGYIWVLGRADEVIKVAGHRLGTYELESALISHPAVAESAVVGV 567
           P   FY+  DY ++D+DGY +VLGR D+VI VAGHRLGT E+E ++ SH AVAE AVVGV
Sbjct: 474 PNAQFYSTFDYGMRDEDGYYFVLGRTDDVINVAGHRLGTREIEESISSHNAVAEVAVVGV 533

Query: 568 PDAIKGEVPIAFVVLKQG--VAPSDELRK---ELREHVRRTIGPIAEPAQIFFVTKLPKT 622
            D +KG+V +AF ++K    VA SD  +K   E+ + V   +G +A P+++ FVT LPKT
Sbjct: 534 ADQLKGQVAVAFAIVKDAALVATSDSAKKLEAEIFKVVDGQLGAVARPSRVHFVTALPKT 593

Query: 623 RSGKIMRRLLKAVATGAPLGDVTTLEDETSVEEAKRA 659
           RSGK++RR ++A+  G   GD+TT+ED  ++++ + A
Sbjct: 594 RSGKLLRRAMQALCEGRDPGDLTTIEDPVALQQLRTA 630


Lambda     K      H
   0.319    0.138    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1178
Number of extensions: 71
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 670
Length of database: 634
Length adjustment: 38
Effective length of query: 632
Effective length of database: 596
Effective search space:   376672
Effective search space used:   376672
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources