Align ABC transporter for Xylitol, ATPase component (characterized)
to candidate WP_024851725.1 N745_RS0108650 ABC transporter ATP-binding protein
Query= reanno::Dino:3607124 (338 letters) >NCBI__GCF_000526715.1:WP_024851725.1 Length = 282 Score = 152 bits (384), Expect = 1e-41 Identities = 93/225 (41%), Positives = 133/225 (59%), Gaps = 16/225 (7%) Query: 4 IKIDKINKFY----GTTQALFDINLDIEDGEFVVFVGPSGCGKSTLLRTLAGLEGVSSGR 59 I I +NK + G + L I+L +++ EFV VG SGCGKSTLLR + GLE ++ G+ Sbjct: 5 IIISGLNKTFITKKGAVEVLESIDLTVKENEFVALVGASGCGKSTLLRIIGGLEKLTKGQ 64 Query: 60 IEIGGRDVTTVEPADRDLAMVFQSYALYPHMTVRENMEFG--MKVNGFEPDLRK-----E 112 + + D V +D AMVFQ Y+LYP +TV+EN++F +K+NG L + Sbjct: 65 VLV---DEEIVNKPGKDRAMVFQDYSLYPWLTVKENIQFSRNLKINGQGNGLGDIGSMVD 121 Query: 113 RIAEAARVLQLEDYLDRKPGQLSGGQRQRVAIGRAIVKNPSVFLFDEPLSNLDAKLRVQM 172 R ++ LE D P QLSGG RQRVAI RA++ P V L DEP LDA+ R M Sbjct: 122 RSYALLDLMGLEKVQDSHPNQLSGGMRQRVAIARALMSKPDVLLMDEPFGALDAQTREVM 181 Query: 173 RVELEGLHKQLGATMIYVTHDQVEAMTMADKIVVL--NRGRIEQV 215 + L + +T+++VTHD EA+ +AD++VV+ N GRI+ + Sbjct: 182 HDLILHLFEIEKSTIVFVTHDVEEAIYLADRVVVMAPNPGRIDSI 226 Lambda K H 0.320 0.139 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 266 Number of extensions: 14 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 338 Length of database: 282 Length adjustment: 27 Effective length of query: 311 Effective length of database: 255 Effective search space: 79305 Effective search space used: 79305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory