Alignments for a candidate for lacZ in Haloglycomyces albus DSM 45210

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacZ in Haloglycomyces albus DSM 45210

Align Exo-alpha-(1->6)-L-arabinopyranosidase; APY; Beta-D-galactopyranosidase; EC 3.2.1.- (characterized)
to candidate WP_025272094.1 HALAL_RS0100400 glycoside hydrolase family 3 C-terminal domain-containing protein

Query= SwissProt::E7CY69
         (757 letters)



>NCBI__GCF_000527155.1:WP_025272094.1
          Length = 806

 Score =  228 bits (582), Expect = 7e-64
 Identities = 144/405 (35%), Positives = 210/405 (51%), Gaps = 23/405 (5%)

Query: 12  LTLEEKASLTSGGDAWHLQGVESKGIPSYMITDGPHGLRKSLASSAGETDLDDSVPATCF 71
           LTLE+K SL SG D W LQ +   G+ +  ++DGP G+R    +SA         P+   
Sbjct: 15  LTLEQKISLLSGIDNWRLQALPHIGLRTITLSDGPIGVRGRYWTSAD--------PSLAL 66

Query: 72  PPAAGLSSSWNPELIHKVGEAMAEECIQEKVAVILGPGVNIKRNPLGGRCFEYWSEDPYL 131
           P     +++W+  LI ++G  +A+E  ++ V V+L P VN++R PLGGR FE +SEDPYL
Sbjct: 67  PSPTAQAATWDTTLIRRIGHLLAQEAHRKNVDVLLAPTVNMQRTPLGGRHFENFSEDPYL 126

Query: 132 AGHEAIGIVEGVQSKGVGTSLKHFAANNQETDRLRVDARISPRALREIYFPAFEHIVKKA 191
            G      V GVQ  G+ T++KH+ AN+ ET+R  V+  I  R LRE Y   FE I + A
Sbjct: 127 TGRIGTAYVTGVQEGGIATTMKHYVANDAETERFTVNNIIDQRTLRETYLRPFEMITRHA 186

Query: 192 QPWTIMCSYNRINGVHSAQNHWLLTDVLRDEWGFDGIVMSDWGADHDRGASLNAGLNLEM 251
           +PW +M +YN +NG  +  +H  L +++R E GFDG  +SDW A  D   ++ AGL+L M
Sbjct: 187 RPWGLMTAYNSVNGT-TMTSHQPLMNLIRHECGFDGFNVSDWTAARDAEHNIEAGLDLAM 245

Query: 252 PPSYT--DDQIVYAVRDGLITPAQLDRMAQGMIDLVNKTRAAMSIDNYR-FDVDAHDEVA 308
           P   T  +  +   +R G I    LD   + ++ L  +  A    +     ++   +   
Sbjct: 246 PGPNTPYNQHLATEIRAGNIDINHLDTAVRHVLTLAARVGALDGTNTTTPPEIPQPETFL 305

Query: 309 HQAAIESIVMLKNDDAILPLNAGPVANPSATPQKIAVIGEFARTPRYQGGGSSHITPTKM 368
              A +S V+ +ND+  LPLN          P KIA+IG  AR  R  GGGS+ + PT +
Sbjct: 306 RSIARQSFVLARNDNNTLPLNRN-------DPGKIALIGPAAREARVLGGGSATVFPTHI 358

Query: 369 TSFLD----TLAERGIKADFAPGFTLDLEPADPALESEAVETAKN 409
            S LD     L +  I        T +L PA    E      AK+
Sbjct: 359 VSPLDGLTHALGDNTIDYHQGVDLTEELSPASEGAELTVTAVAKD 403



 Score =  173 bits (439), Expect = 3e-47
 Identities = 112/275 (40%), Positives = 144/275 (52%), Gaps = 17/275 (6%)

Query: 396 DPALESEAVETAKNADVVLMFLGLPEAVESEGFDRDTLDMPAKQIALLEQVAAANQNVVV 455
           D  L +EA   AKNAD  ++ +   E  ESEG+DR  LD+P +Q  L+  VA AN N +V
Sbjct: 534 DHELIAEAATAAKNADTAIIVVATTELTESEGWDRTNLDLPGRQNDLVTAVAQANPNTIV 593

Query: 456 VLSNGSVITVAPWAKNAKGILESWLLGQSGGPALADVIFGQVSPSGKLAQSIPLDINDDP 515
           V++ GS +T+ PW  +   IL +W  GQ GG ALADV+ G   P G+L  + P    D P
Sbjct: 594 VVNAGSPVTM-PWRNDVPAILLTWFPGQEGGHALADVLLGS-EPGGRLPATWPATTEDAP 651

Query: 516 SMLNWPGEEGHVDYGEGVFAGYRYYDTYGKAVD-YPFGYGLSYATFEITGVAVAKTGANT 574
            +   P  +G + Y EGVF GYR ++  G     Y FG+GLSY T+E   +AV     NT
Sbjct: 652 VLNAKPNADGDLHYEEGVFIGYRAWEKRGTPQPAYWFGHGLSYTTWEYESLAVE---GNT 708

Query: 575 ATVTATVTNTSDVDAAETVQVYVVPG-----KADVARPKHELKGFTKAFLKAGESKTVAI 629
           A V  TV N+      E VQVYV P      K D    KH L GFT       ES TV +
Sbjct: 709 ARV--TVRNSGSRSGREVVQVYVGPDADTAEKIDGGIEKHRLAGFTTLEAAPNESTTVEV 766

Query: 630 DLDERAF----AYWSEKYNDWHVEAGEYAIEVGVS 660
           +LDE AF      W+     W V AG    ++ VS
Sbjct: 767 ELDEHAFDVFDGEWTTVSGGWQVSAGRSVADLRVS 801


Lambda     K      H
   0.315    0.132    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1445
Number of extensions: 84
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 757
Length of database: 806
Length adjustment: 41
Effective length of query: 716
Effective length of database: 765
Effective search space:   547740
Effective search space used:   547740
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources