Alignments for a candidate for glk in Thiothrix lacustris DSM 21227

GapMind for catabolism of small carbon sources

Alignments for a candidate for glk in Thiothrix lacustris DSM 21227

Align Glucokinase; EC 2.7.1.2; Glucose kinase (uncharacterized)
to candidate WP_028490344.1 Q394_RS0117280 glucokinase

Query= curated2:Q55855
         (355 letters)



>NCBI__GCF_000621325.1:WP_028490344.1
          Length = 336

 Score =  227 bits (578), Expect = 4e-64
 Identities = 134/341 (39%), Positives = 185/341 (54%), Gaps = 18/341 (5%)

Query: 9   LAGDIGGTKTILALVTINESSPGLARPVTLFEQTYSSPAFPDLVPMVQQFRQEAAFVLGN 68
           LAGD+GGTKT+L L  +N + P       + +  Y S  +     +V  F  + A     
Sbjct: 5   LAGDVGGTKTVLCLYRVNAAKPDGTSLEEVHKAVYPSSGYERFGDLVTSFLTDVA----- 59

Query: 69  PISVAKACFAIAGPVIDNTCRLTNLDWHLSGDRLAQELAIAQVDLINDFAAVGYGILGLG 128
           P+    ACF IAGP+    C  TNL W +    LA++     V LIND  A  YG+L L 
Sbjct: 60  PLRPECACFGIAGPIQQQRCNATNLPWVIDAGILAEDFGFEDVYLINDLEAAAYGMLHLA 119

Query: 129 SEDLTVLQAAPVDPSGAIAILGAGTGLGQCYVIPQGQGRYRVFASEGAHGDFAPRSPLEW 188
           +++   L       +G  A++ AGTGLGQ  +   GQ R+ V  +EG H DF   S  E 
Sbjct: 120 ADEFVELNPHAAPQTGHAAVIAAGTGLGQAIMAWDGQ-RHIVMPTEGGHCDFGANSSQED 178

Query: 189 QLLEYLKKKYSLGRISIERVVSGMGIAMIYEFLRHQYPERESAQFSKLYQTWNREKDQET 248
            LL +L++++  G +S ER+++G G   +Y+FLR           +  +   N   + E 
Sbjct: 179 ALLVFLRERFG-GHVSGERILAGDGFGNLYDFLR-----------ASNHAAPNAAIEAEM 226

Query: 249 KTVDLAAAVSQAALEGTDVLADQAMELFLGAYGAEAGNLALKLLPRGGLYVAGGIAPKII 308
              D  A +S+  L+G D L  + M LF+  YG+EAGNLALK LPRGG+Y+ GGIAPKI 
Sbjct: 227 LHEDRNAVISRHGLQGDDPLCTEVMRLFVRIYGSEAGNLALKCLPRGGIYIGGGIAPKIR 286

Query: 309 PLLEKGSFMQGFSDKGRMQSLMGTIPVQVVLNAKVGLIGAA 349
             LE G FMQGF DKGRM   +  IPV++ LN +  L+GAA
Sbjct: 287 AALETGLFMQGFLDKGRMARAIEHIPVRLSLNPEAPLLGAA 327


Lambda     K      H
   0.320    0.137    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 312
Number of extensions: 17
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 355
Length of database: 336
Length adjustment: 29
Effective length of query: 326
Effective length of database: 307
Effective search space:   100082
Effective search space used:   100082
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_028490344.1 Q394_RS0117280 (glucokinase)
to HMM TIGR00749 (glk: glucokinase (EC 2.7.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00749.hmm
# target sequence database:        /tmp/gapView.895898.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00749  [M=315]
Accession:   TIGR00749
Description: glk: glucokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    9.4e-77  244.3   0.0    1.1e-76  244.1   0.0    1.0  1  NCBI__GCF_000621325.1:WP_028490344.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000621325.1:WP_028490344.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  244.1   0.0   1.1e-76   1.1e-76       1     315 []       5     327 ..       5     327 .. 0.90

  Alignments for each domain:
  == domain 1  score: 244.1 bits;  conditional E-value: 1.1e-76
                             TIGR00749   1 lvgdiGGtnarlalvevapgeieqv.......ktyssedfpsleavvrvyleeakvelkdpikgcfaiatPii 66 
                                           l+gd+GGt+++l+l  v + + +          +y s  ++ + ++v  +l + +     p  +cf ia+Pi 
  NCBI__GCF_000621325.1:WP_028490344.1   5 LAGDVGGTKTVLCLYRVNAAKPDGTsleevhkAVYPSSGYERFGDLVTSFLTDVAPL--RPECACFGIAGPIQ 75 
                                           79************94443332222223347789*****************998765..4669********** PP

                             TIGR00749  67 gdfvrltnldWalsieelkqelalaklelindfaavayailalkeedliqlggakveesaaiailGaGtGlGv 139
                                           ++ ++ tnl W +    l+++++++ + lind+ a ay++l l  ++++ l+   + +++  a++ aGtGlG+
  NCBI__GCF_000621325.1:WP_028490344.1  76 QQRCNATNLPWVIDAGILAEDFGFEDVYLINDLEAAAYGMLHLAADEFVELNPHAAPQTGHAAVIAAGTGLGQ 148
                                           ******************************************************999**************** PP

                             TIGR00749 140 atliqqsdgrykvlageGghvdfaPrseleillleylrkky.grvsaervlsGsGlvliyealskrkgerevs 211
                                           a +  +++ r+ v+++eGgh df  +s +e  ll +lr+++ g+vs er+l+G G+ ++y++l+  +     +
  NCBI__GCF_000621325.1:WP_028490344.1 149 AIMAWDGQ-RHIVMPTEGGHCDFGANSSQEDALLVFLRERFgGHVSGERILAGDGFGNLYDFLRASNHAAPNA 220
                                           *9999665.7889****************************99********************9776555544 PP

                             TIGR00749 212 klsk...eelkekdiseaalegsdvlarralelflsilGalagnlalklgarGGvyvaGGivPrfiellkkss 281
                                            +     +e ++  is+  l+g+d+l+ + + lf+ i+G++agnlalk ++rGG+y+ GGi+P++   l+++ 
  NCBI__GCF_000621325.1:WP_028490344.1 221 AIEAemlHEDRNAVISRHGLQGDDPLCTEVMRLFVRIYGSEAGNLALKCLPRGGIYIGGGIAPKIRAALETGL 293
                                           44440004556667*********************************************************** PP

                             TIGR00749 282 fraafedkGrlkellasiPvqvvlkkkvGllGag 315
                                           f+++f dkGr+   +++iPv++ l+ ++ llGa+
  NCBI__GCF_000621325.1:WP_028490344.1 294 FMQGFLDKGRMARAIEHIPVRLSLNPEAPLLGAA 327
                                           ********************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (315 nodes)
Target sequences:                          1  (336 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 23.83
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources