Alignments for a candidate for fumD in Thiothrix lacustris DSM 21227

GapMind for catabolism of small carbon sources

Alignments for a candidate for fumD in Thiothrix lacustris DSM 21227

Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate WP_028487649.1 Q394_RS0101065 fumarate hydratase

Query= BRENDA::Q141Z6
         (520 letters)



>NCBI__GCF_000621325.1:WP_028487649.1
          Length = 505

 Score =  744 bits (1922), Expect = 0.0
 Identities = 367/496 (73%), Positives = 425/496 (85%), Gaps = 2/496 (0%)

Query: 14  MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAE 73
           MT+IKQ D+I S+AD+LQ+ISYYHP D+I+A+ +A+E E+SPAAKD+IAQIL NSRMCAE
Sbjct: 1   MTIIKQNDVIASVADALQFISYYHPADFIEAMHKAWEREESPAAKDSIAQILVNSRMCAE 60

Query: 74  GKRPICQDTGIVTVFVKVGMDVRWDGATMGVTDMINEGVRRGYLNPDNVLRASIVSPPEG 133
           G RPICQDTGIVTVFVKVGM+V+W+G  + ++DMINEGVRR Y+NPDNVLRASI++ P G
Sbjct: 61  GHRPICQDTGIVTVFVKVGMNVQWEG-DLSLSDMINEGVRRAYMNPDNVLRASILADPAG 119

Query: 134 GRKNTKDNTPAVIHYEIVPGNTVDVQVAAKGGGSENKSKFAMLNPSDSIVDWILKTVPTM 193
            RKNTKDNTPAVIHYEIVPG+ V   VAAKGGGSENKSK  MLNPSDSIVDW+LKTVPTM
Sbjct: 120 ARKNTKDNTPAVIHYEIVPGDKVSFDVAAKGGGSENKSKMVMLNPSDSIVDWVLKTVPTM 179

Query: 194 GAGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNA 253
           GAGWCPPGMLGIGIGGTAEKA V+AKE LM+PIDIQD+IARGP++ +EELR+EL+EKVN 
Sbjct: 180 GAGWCPPGMLGIGIGGTAEKAAVLAKEVLMEPIDIQDLIARGPQNRVEELRLELYEKVNQ 239

Query: 254 LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGAARLEAPSL 313
           LGIGAQGLGGL TVLDVKI   PTHAAS PV +IPNCAATRH HF LDGSG A    P L
Sbjct: 240 LGIGAQGLGGLTTVLDVKIKDYPTHAASLPVCMIPNCAATRHTHFVLDGSGPALQTPPDL 299

Query: 314 DAWPKVHWQPDTEKSKRVDLNTLTPEEVAAWTPGQTLLLSGKMLTGRDAAHKRIADMLAK 373
             WP++      +++ RV+L+T+TPE+V    PG+T+LLSGKMLTGRDAAHKR+ DML K
Sbjct: 300 ATWPQIS-LAGGDQATRVNLDTITPEDVRKLKPGETVLLSGKMLTGRDAAHKRMIDMLNK 358

Query: 374 GEKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTEMMLAQTGLISMIGKAER 433
           GE+LPVD   R IYYVGPVDPVR+E VGPAGPTT+TRMDKFT  +L QTGL+ MIGK+ER
Sbjct: 359 GEQLPVDLKGRFIYYVGPVDPVREEVVGPAGPTTSTRMDKFTRQILDQTGLLGMIGKSER 418

Query: 434 GPVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAV 493
           GP+AIEAI++  A YLMAVGGAAYLVSKAI  AKVLAF +LGMEAIYEF+V+DMPVTVAV
Sbjct: 419 GPIAIEAIKEFGAVYLMAVGGAAYLVSKAIVGAKVLAFPELGMEAIYEFEVKDMPVTVAV 478

Query: 494 DSNGTSVHQTGPKEWQ 509
           DSNG SVH TGP +W+
Sbjct: 479 DSNGESVHNTGPAQWK 494


Lambda     K      H
   0.316    0.133    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 938
Number of extensions: 32
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 505
Length adjustment: 35
Effective length of query: 485
Effective length of database: 470
Effective search space:   227950
Effective search space used:   227950
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources