Alignments for a candidate for pccA1 in Thiothrix lacustris DSM 21227

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccA1 in Thiothrix lacustris DSM 21227

Align acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized)
to candidate WP_028489135.1 Q394_RS0109800 acetyl-CoA carboxylase biotin carboxylase subunit

Query= metacyc::MONOMER-13597
         (509 letters)



>NCBI__GCF_000621325.1:WP_028489135.1
          Length = 447

 Score =  386 bits (991), Expect = e-111
 Identities = 212/444 (47%), Positives = 285/444 (64%), Gaps = 6/444 (1%)

Query: 6   RVLVANRGEIATRVLKAIKEMGMTAIAVYSEADKYAVHTKYADEAYYIGKAPALDSYLNI 65
           +VL+ANRGEIA R+L+  +E+G+  +AV+S AD+   H + ADE+  IG   + DSYLNI
Sbjct: 4   KVLIANRGEIALRILRGCRELGIKTVAVHSTADRDLKHVRLADESVCIGPPRSTDSYLNI 63

Query: 66  EHIIDAAEKAHVDAIHPGYGFLSENAEFAEAVEKAGITFIGPSSEVMRKIKDKLDGKRLA 125
             II AAE    DAIHPGYGFLSENA+FAE VE +G  FIGP ++ +R + DK+  K   
Sbjct: 64  PAIISAAEVTGADAIHPGYGFLSENADFAERVESSGFIFIGPRADTIRLMGDKISAKDAM 123

Query: 126 NMAGVPTAPGSDGPV-TSIDEALKLAEKIGYPIMVKAASGGGGVGITRVDNQDQLMDVWE 184
             AGVP  PGS+G      +E LK+  +IGYP++VKA  GGGG G+  V   D+L+    
Sbjct: 124 IAAGVPCVPGSEGGTPEDPEEILKMGTRIGYPLIVKATGGGGGRGMRVVHTADELVAAVA 183

Query: 185 RNKRLAYQAFGKADLFIEKYAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKLIEEA 244
             +  A  AFG   +F+EK+  +PRHIE Q++ D +GN +   ER+C++QRRNQK++EEA
Sbjct: 184 LTRSEARAAFGNDIVFMEKFLQHPRHIELQVLADSHGNAIHLCERDCSMQRRNQKVVEEA 243

Query: 245 PSPALKMEERESMFEPIIKFGKLINYFTLGTFETAFSDVSRDFYFLELNKRLQVEHPTTE 304
           P+P +  E+R  +   + +  + I Y   GTFE  + D   +FYF+E+N RLQVEHP TE
Sbjct: 244 PAPFITEEQRARIGNRVAEACRKIGYRGAGTFEFLYED--GEFYFIEMNTRLQVEHPVTE 301

Query: 305 LIFRIDLVKLQIKLAAGEHLPFSQEDLNKRVRGTAIEYRINAEDALNNFTGSSGFVTYYR 364
           LI  +DLVK Q+ +A+GE L   QED+  +V G AIE RINAED    F  S G +T Y 
Sbjct: 302 LITGVDLVKQQLLIASGEVLALRQEDI--KVNGHAIECRINAEDP-QTFAPSPGKITRYH 358

Query: 365 EPTGPGVRVDSGIESGSYVPPYYDSLVSKLIVYGESREYAIQAGIRALADYKIGGIKTTI 424
            P G GVRVDS I +   VPPYYDS++ KLIV+G+ RE AI     AL++  I GIKT I
Sbjct: 359 VPGGLGVRVDSHIYADYSVPPYYDSMIGKLIVHGQDRETAINRMHGALSEMVIEGIKTNI 418

Query: 425 ELYKWIMQDPDFQEGKFSTSYISQ 448
            L   IM++P F+ G     Y+ +
Sbjct: 419 PLQTRIMENPTFRAGGADIHYLEK 442


Lambda     K      H
   0.317    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 551
Number of extensions: 13
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 509
Length of database: 447
Length adjustment: 33
Effective length of query: 476
Effective length of database: 414
Effective search space:   197064
Effective search space used:   197064
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources