Alignments for a candidate for prpE in Thiothrix lacustris DSM 21227

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Thiothrix lacustris DSM 21227

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_028489625.1 Q394_RS0112815 acetate--CoA ligase

Query= SwissProt::P27550
         (652 letters)



>NCBI__GCF_000621325.1:WP_028489625.1
          Length = 648

 Score =  827 bits (2137), Expect = 0.0
 Identities = 390/648 (60%), Positives = 489/648 (75%), Gaps = 4/648 (0%)

Query: 1   MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK-ILDWIKPYQKVKNTS 59
           MS +  + +PA  A +  IN +QY +MY QSIN PDTFWGEQ +  L W + + KV++ S
Sbjct: 1   MSDVKTYPVPAEFAAQANINAEQYASMYAQSINDPDTFWGEQAEQYLTWFQKWDKVQDWS 60

Query: 60  FAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDASQSKHISYKELHRDVCR 119
           F   ++ I W+  GTLN++ NCLDRHL   GD+ AIIWEGD+ ++ + I+Y+ELH +V +
Sbjct: 61  FDQDDLHINWFTGGTLNVSYNCLDRHLDTRGDQVAIIWEGDNPNEDRKITYRELHAEVSK 120

Query: 120 FANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSN 179
            AN L   G+K+GD V++Y+PM+PEAAVAMLAC RIGA+HS++FGGFSP+A+  RI DS 
Sbjct: 121 LANVLKARGVKRGDRVSLYLPMIPEAAVAMLACTRIGAIHSIVFGGFSPDALRDRIQDSE 180

Query: 180 SRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHD 239
             +VITSD+ +R G+ +PLK N D A+      SV   +V++R G  + W + RD+W+H+
Sbjct: 181 CGVVITSDQSMRGGKKVPLKGNADKAMDQ--CPSVHTCIVVQRGGDPVKWNDSRDVWYHE 238

Query: 240 LVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGD 299
            +  A  +  AE M A+DPLFILYTSGSTGKPKGVLHTTGGYL+ AA+T K VFDY  G+
Sbjct: 239 AIAAADAECPAEPMEADDPLFILYTSGSTGKPKGVLHTTGGYLLQAAMTHKTVFDYKDGE 298

Query: 300 IYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPT 359
           +YWCTADVGWVTGHSY++YGPLA GAT+LMFEG+P +P   R  QV DKH V   YTAPT
Sbjct: 299 VYWCTADVGWVTGHSYIVYGPLANGATSLMFEGIPTYPDAGRFWQVCDKHNVATFYTAPT 358

Query: 360 AIRALMAEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETG 419
           AIR LM  GD+ +E    SSLR+LGSVGEPINPEAWEWY + +G  +CP+VDTWWQTETG
Sbjct: 359 AIRMLMGAGDQFVEKAQLSSLRLLGSVGEPINPEAWEWYHRVVGKNRCPIVDTWWQTETG 418

Query: 420 GFMITPLPGATELKAGSATRPFFGVQPALVDNEGNPLEG-ATEGSLVITDSWPGQARTLF 478
             M+TPLPGAT LK GSAT PFFGV+P L+D++GN +EG    G+L I   WP   R+L+
Sbjct: 419 AHMLTPLPGATPLKPGSATHPFFGVEPCLLDDQGNEVEGNPAVGNLAIKRPWPSMMRSLY 478

Query: 479 GDHERFEQTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVA 538
           GDH+RF + YF+ FK  YF+GDGARRDEDGYYWITGRVDDVLN+SGHRLGTAEIESALV 
Sbjct: 479 GDHKRFYEAYFAMFKGYYFTGDGARRDEDGYYWITGRVDDVLNISGHRLGTAEIESALVL 538

Query: 539 HPKIAEAAVVGIPHNIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHW 598
           HPK+AEAAVVG PH + GQ IYAYVTL  GEE + +L  E+   VRKEIGP+A  +++ W
Sbjct: 539 HPKVAEAAVVGYPHELTGQGIYAYVTLMAGEEGTDDLKNELNTLVRKEIGPIAKINIIQW 598

Query: 599 TDSLPKTRSGKIMRRILRKIAAGDTSNLGDTSTLADPGVVEKLLEEKQ 646
           +  LPKTRSGKIMRRILRKIAA +   LGDTSTLADP VV+ L++ ++
Sbjct: 599 SPGLPKTRSGKIMRRILRKIAANEIDGLGDTSTLADPSVVDNLIDFRE 646


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1444
Number of extensions: 65
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 648
Length adjustment: 38
Effective length of query: 614
Effective length of database: 610
Effective search space:   374540
Effective search space used:   374540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources