Alignments for a candidate for mtlE in Thiothrix lacustris DSM 21227

GapMind for catabolism of small carbon sources

Alignments for a candidate for mtlE in Thiothrix lacustris DSM 21227

Align ABC transporter for D-Sorbitol, periplasmic substrate-binding component (characterized)
to candidate WP_028488081.1 Q394_RS0103645 sugar ABC transporter substrate-binding protein

Query= reanno::acidovorax_3H11:Ac3H11_2944
         (443 letters)



>NCBI__GCF_000621325.1:WP_028488081.1
          Length = 439

 Score =  690 bits (1781), Expect = 0.0
 Identities = 326/435 (74%), Positives = 375/435 (86%)

Query: 9   TRLALAAAALAASLCGAAAHAQTELVIATVNNGHMIEMQKLSKNFEQAHPDIKLKWVTLE 68
           TRL  A +          +HA TELV+ATVNNGHM+EMQKLSK+FE A+PDIKLKWVTLE
Sbjct: 4   TRLFTAISGAILLTSATLSHAATELVVATVNNGHMVEMQKLSKHFEDANPDIKLKWVTLE 63

Query: 69  EGVLRQRVTTDIATKGGQFDVMTIGMYEAPIWGKKGWLQELKTDAAYDVDDLLPAVRNGL 128
           EGVLRQRVTTDIATKGGQFDVMTIGMYEAPIWGKKGW+++L  D AYDV D+LPA+R+GL
Sbjct: 64  EGVLRQRVTTDIATKGGQFDVMTIGMYEAPIWGKKGWIKDLTFDDAYDVGDILPAIRDGL 123

Query: 129 SIDGKLFAAPFYGESSMLMYRKDLADKAGVQVPERPTWPQIKDLAAKIHDPKNGVYGICL 188
           S DGKL+AAPFYGESSMLMYRKDL +KAG+ +P+ PTW  + ++AAKI D  NGVYGICL
Sbjct: 124 SADGKLYAAPFYGESSMLMYRKDLVEKAGMDMPDNPTWDHVAEVAAKITDKDNGVYGICL 183

Query: 189 RGKPGWGDNMAFLSTLVNTFGGQWFDMQWKPQLESKPWKEAITFYVDLLKNYGPPGSSAN 248
           RGKPGWGDNMA ++T+ NTFGGQWFDM WKPQLESKPWK+A+ FYVDL+  YGPPGS++N
Sbjct: 184 RGKPGWGDNMALITTMANTFGGQWFDMDWKPQLESKPWKDAVGFYVDLMSKYGPPGSASN 243

Query: 249 SFNEILALTNSGKCGMWIDATIAASFVSDPKQSKVADQMAFAQAPTMNTPKGANWLWSWN 308
           SFNEILAL N GKCGMW+DATIAASF++DP QSKVAD++AFAQAPT  TPKGANWLW+W 
Sbjct: 244 SFNEILALYNEGKCGMWVDATIAASFITDPAQSKVADKVAFAQAPTKATPKGANWLWAWA 303

Query: 309 LAIPAGSKKVDAAQKFITWSTSKEYVQLVAKTNGWANVPTGTRKSTYASPEFQKAARFAA 368
           LA+P  SK    AQKFI WSTSK+Y+ LVAK  GW+ VPTGTR STYA+ +FQKAA FA+
Sbjct: 304 LAVPESSKHSAEAQKFIEWSTSKDYINLVAKEEGWSKVPTGTRASTYANADFQKAAVFAS 363

Query: 369 AEKVAIDSANPTDSTLPKSPYVGVQFAAIPEFQAIGIAVGQQMSAALAGKTTVDAALKAS 428
           AEK AIDSANP DSTLPKSPY GVQFAAIPEFQAIGIAVGQQ+S+ALAGK +VD ALKAS
Sbjct: 364 AEKTAIDSANPNDSTLPKSPYTGVQFAAIPEFQAIGIAVGQQLSSALAGKMSVDDALKAS 423

Query: 429 QVSADREMKKAGYYK 443
           QVSA+REM+++GY+K
Sbjct: 424 QVSAEREMQRSGYFK 438


Lambda     K      H
   0.316    0.129    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 659
Number of extensions: 16
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 443
Length of database: 439
Length adjustment: 32
Effective length of query: 411
Effective length of database: 407
Effective search space:   167277
Effective search space used:   167277
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources