GapMind for catabolism of small carbon sources

 

Alignments for a candidate for ecfA1 in Thiothrix lacustris DSM 21227

Align Energy-coupling factor transporter ATP-binding protein EcfA1; Short=ECF transporter A component EcfA; EC 7.-.-.- (characterized, see rationale)
to candidate WP_028488909.1 Q394_RS0108480 spermidine/putrescine ABC transporter ATP-binding protein PotA

Query= uniprot:P40735
         (281 letters)



>NCBI__GCF_000621325.1:WP_028488909.1
          Length = 369

 Score =  130 bits (326), Expect = 6e-35
 Identities = 82/237 (34%), Positives = 136/237 (57%), Gaps = 7/237 (2%)

Query: 1   MNQNQLISVEDIVFRYRKDAERRALDGVSLQVYEGEWLAIVGHNGSGKSTLARALNGLIL 60
           M ++ ++++ +I  R+   A +  L   +L + +GE+  I+G +G GK+T+ R + G   
Sbjct: 1   MTRSPILTLSNISKRF---ASQEVLSDFNLTIQDGEFFTILGPSGCGKTTVLRLIAGFEQ 57

Query: 61  PESGDIEVAGIQLTEESVWEVRKKIGMVFQNPDNQFVGTTVRDDVAFGLENNGVPREEMI 120
           P  G I + G  +    +   ++    VFQ+    F   TV D+VAFGL+  GV  +++ 
Sbjct: 58  PNEGQILLNGDDIAR--IPAEKRPFNTVFQSYA-LFPHLTVFDNVAFGLKMAGVDVQDIA 114

Query: 121 ERVDWAVKQVNMQDFLDQEPHHLSGGQKQRVAIAGVIAARPDIIILDEATSMLDPIGREE 180
            RV  A+  V + +F  ++PH LSGGQKQRVAIA  +  RP I++LDE+ S LD   R++
Sbjct: 115 VRVADALAIVRLSEFATRKPHQLSGGQKQRVAIARAVVNRPKILLLDESLSALDYKLRQQ 174

Query: 181 VLETVRHLKEQGMATVISITHDLNEA-AKADRIIVMNGGKKYAEGPPEEIFKLNKEL 236
           +   ++ L+ Q   T + +THD  EA + +DRI+VM+ G+    G P EI++  + L
Sbjct: 175 MQLELKQLQRQLGITFVYVTHDQEEALSMSDRILVMHNGQAQQVGTPREIYESPRNL 231


Lambda     K      H
   0.316    0.135    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 254
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 281
Length of database: 369
Length adjustment: 28
Effective length of query: 253
Effective length of database: 341
Effective search space:    86273
Effective search space used:    86273
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory