Alignments for a candidate for pimF in Xenophilus azovorans DSM 13620

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Xenophilus azovorans DSM 13620

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate WP_038216456.1 Q392_RS27900 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein

Query= metacyc::MONOMER-20678
         (699 letters)



>NCBI__GCF_000745855.1:WP_038216456.1
          Length = 704

 Score =  652 bits (1683), Expect = 0.0
 Identities = 352/697 (50%), Positives = 472/697 (67%), Gaps = 23/697 (3%)

Query: 13  VAIVTVDSPPVNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADITEFGKPP 72
           VA++T+ +PPVN L  + R GI + ++ A+AD AV+AIVL  AG+ F  GADI EFG P 
Sbjct: 11  VAVITMSNPPVNGLGLSTRVGITDGLSKALADAAVKAIVLTGAGKAFSGGADIKEFGSPK 70

Query: 73  --QPPALNDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEAKLGLPEVKLGL 130
             Q P L  VI ALE S KP +AA+H   +GGGLE+ALGCH+RVA     + LPEVKLGL
Sbjct: 71  ALQDPNLLSVILALEASTKPIVAAVHSICMGGGLELALGCHYRVAAPGTSVALPEVKLGL 130

Query: 131 LPGAGGTQRLPRAVGPELAVQMIVGGSPIGA---AEALKHGLVEEVV---ENLVAGAVAF 184
           +PGAGGTQRLPR +G E A+ +IV G P+ +   A+A    L +++    E+L+  A+AF
Sbjct: 131 IPGAGGTQRLPRVLGVEAALNLIVSGQPVKSELLAKAPGQKLFDKLAASPESLMDEALAF 190

Query: 185 AKKVLAEKRP----LRRLRDDDSKLAAAKADRSIFTNAVAAMTKKARGLEAPFACADAIG 240
           A++V A+       L  +R+   +     A      N V  M+K      AP  C +A+ 
Sbjct: 191 AREVAAKHGQGGGDLPLVRNLPCRHPQGDAYFQFARNMVGGMSKN---FPAPLKCVEAVQ 247

Query: 241 AAIDLPFEEGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPRPVSRVAI 300
           AA  L F+ GL KERE F+ L+V+ +S A R+ F AER A+K+  VP+ T  R +  V +
Sbjct: 248 AATQLKFDLGLAKEREIFLALMVTPESAALRHLFMAERAASKIPDVPEDTPVRAIKTVGV 307

Query: 301 IGAGTMGGGIAMSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLPPDAPAKRM 360
           IGAGTMGGGI+M+F NAGIPVT++E  +E + RG+  ++KN+EA   +G L  D   +RM
Sbjct: 308 IGAGTMGGGISMNFLNAGIPVTILEMKQEAVDRGVATIRKNYEAQVKKGKLKQDKYEERM 367

Query: 361 ALITGLVGLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSYLSIDEIAA 420
           AL+   +  +++KD DLIIEAVFE + VK++VF  +DA AK GA+LASNTS L +D+IAA
Sbjct: 368 ALLKTTLSYDDLKDCDLIIEAVFEELGVKEKVFKQLDAVAKQGAILASNTSTLDVDKIAA 427

Query: 421 TTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVVVGVCDGFV 480
            T+RPQDV+GMHFFSPANVMKL E+VRG  TA D L T + +AKKI K  VV GVCDGF+
Sbjct: 428 FTQRPQDVVGMHFFSPANVMKLLEVVRGKATAKDVLATVMQLAKKIKKTAVVSGVCDGFI 487

Query: 481 GNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWRSRKDRGIK 540
           GNRM+   S+Q+  LL EGA PQQVD  + KFG  MGPF MGDLAG DIGW  RK R ++
Sbjct: 488 GNRMIEQYSRQAGFLLDEGATPQQVDKAIEKFGFAMGPFRMGDLAGNDIGWAIRKRRAVE 547

Query: 541 ------SEIADALCEAGRFGQKTGKGYYKYEQGSRAPMPDPEVETLINDTLAKLGLKRRD 594
                 S  AD LCE GRFGQKTG G+Y Y+ G R  +P   V+ +I +   +LG+  R 
Sbjct: 548 QPDMKYSRTADKLCELGRFGQKTGAGWYDYQAGKRDAIPSELVDKMIEEHRKELGITPRK 607

Query: 595 ITDEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGPMHYADSVGLK 654
           I+DEEI++R+V+ ++NEGA ILE+ IA++  DID+V+L GYG+P++RGGPMHYA  VGL 
Sbjct: 608 ISDEEIVQRLVFSLVNEGAHILEDGIASKAGDIDMVYLTGYGFPMHRGGPMHYASQVGLY 667

Query: 655 HIAERLSAYAKATNDPS--LEPAPLLARLAAEGKTFA 689
           ++AE +  +A+  +D +   EPAPL+ +L AEGK+F+
Sbjct: 668 NVAEAMKRFARNPHDDAKFWEPAPLIQKLVAEGKSFS 704


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1085
Number of extensions: 54
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 704
Length adjustment: 39
Effective length of query: 660
Effective length of database: 665
Effective search space:   438900
Effective search space used:   438900
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources