Alignments for a candidate for leuT in Desulfobacter vibrioformis DSM 8776

GapMind for catabolism of small carbon sources

Alignments for a candidate for leuT in Desulfobacter vibrioformis DSM 8776

Align The amino acid (leucine):2 Na+ symporter, LeuTAa (Yamashita et al., 2005). LeuT possesses two ion binding sites, NA1 and NA2, both highly specific for Na+ but with differing mechanisms of binding (Noskov and Roux, 2008). X-ray structures have been determined for LeuT in substrate-free outward-open and apo inward-open states (characterized)
to candidate WP_035240196.1 Q366_RS14330 sodium-dependent transporter

Query= TCDB::O67854
         (513 letters)



>NCBI__GCF_000745975.1:WP_035240196.1
          Length = 490

 Score =  233 bits (595), Expect = 9e-66
 Identities = 162/472 (34%), Positives = 239/472 (50%), Gaps = 40/472 (8%)

Query: 4   KREHWATRLGLILAMAGNAVGLGNFLRFPVQAAENGGGAFMIPYIIAFLLVGIPLMWIEW 63
           KRE W TR G ILA  G+A+GLGN  RFP  A ENGGGAF IPY+ A L  GIP + +E+
Sbjct: 3   KREQWGTRAGFILAAIGSAIGLGNIWRFPYVAYENGGGAFFIPYLFAMLTAGIPFLILEF 62

Query: 64  AMGRYGGAQGHGTTPAIFYLL---WRNRFAKILGVFGLWIPLVVAIYYVYIESWTLG-FA 119
            +G     +   + P IF  L   W     + LG + L +  +++IYYV +  W++  F 
Sbjct: 63  GVGH----KFKTSVPNIFRSLSGRW-----EWLGWWQLLVAFIISIYYVAVVGWSISYFV 113

Query: 120 IKFLVGLVPEPPPNATDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFIN 179
           + F  G          DP +    FK +L      P     I  P   A    L   FI 
Sbjct: 114 LAFTRGW-------GADPANFF--FKTYL-QLSDTPFAFNGIRWPIFGA---ILCAWFIC 160

Query: 180 VSILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLK 239
             +L  G+ KGIE  +KI MP LFI+ + +  R   L+     A +GLN+++ PDF  L 
Sbjct: 161 WGVLFSGVKKGIESASKIFMPLLFIMVLIITARAVTLD----GAKEGLNWMFKPDFSALL 216

Query: 240 DPGVWIAAVGQIFFTLSLGFGAIITYASYVRKDQDIVLSGLTAATLNEKAEVILGGSI-S 298
           +  VWIAA GQ+FF+LS+GF  ++TYASY+ +D D+  +G   A  N    ++ G  + S
Sbjct: 217 NFKVWIAAYGQLFFSLSIGFAIMLTYASYLPEDSDMANNGFITAFCNCGFSILCGIMVFS 276

Query: 299 IPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLTSSIA 358
           +   +AF        +  +G   L F+T+P   +   G  F G L+F  L FAGL+S ++
Sbjct: 277 VLGNMAFQQGVGVDKVVSSGV-GLAFVTIPTAINSLPGPVFFGTLFFAALLFAGLSSMVS 335

Query: 359 IMQPMIAFLEDELKLSRKHAVLWTAAI-----VFFSAHLVMFLNKSLDEMDFWAGTIGVV 413
           + +  ++ L D   +SRK A      I     + F++H  + +   LD +D +    GV+
Sbjct: 336 VCEVSVSALIDRFGISRKIAASLYCGIGILCGIVFASHSGLLV---LDIVDRFINNFGVL 392

Query: 414 FFGLTELIIFFWIFGADKAWEEINRGGIIKVPRIYYYVMRYITPAFLAVLLV 465
             GL E+I   WI G D     IN     KV  ++ + ++ ITPA L  + +
Sbjct: 393 AGGLVEIIFLAWICGLDGFKGIINLTSDFKVGTLWAFCLKIITPAVLGYMSI 444


Lambda     K      H
   0.330    0.146    0.463 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 747
Number of extensions: 41
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 513
Length of database: 490
Length adjustment: 34
Effective length of query: 479
Effective length of database: 456
Effective search space:   218424
Effective search space used:   218424
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources