Align glycolaldehyde oxidoreductase small subunit (characterized)
to candidate WP_036260175.1 DL86_RS08520 (2Fe-2S)-binding protein
Query= metacyc::MONOMER-18073 (163 letters) >NCBI__GCF_000746085.1:WP_036260175.1 Length = 152 Score = 127 bits (320), Expect = 6e-35 Identities = 62/143 (43%), Positives = 89/143 (62%) Query: 12 IKVKVNGVLYERYVSPRILLVDFLREELGLTGTKIGCDTTTCGACTVLLNGKSVKSCTLF 71 + ++VN ++ P + LV LR+ +GL GTK GC CGAC V L+G++V++C Sbjct: 2 LDLRVNDAAHQVDAPPEMPLVWVLRDLIGLRGTKFGCGIGACGACIVHLDGQAVRACITT 61 Query: 72 AVQADGAEITTIEGLSVDSKLHPIQEAFKENFALQCGFCTPGMIMQAYFLLKENPNPSEE 131 A +A G ITTIEGL+ H +Q A+K+ A QCG+C PG +MQA LL + P P++E Sbjct: 62 AAEAHGKAITTIEGLAGAHGDHSLQRAWKQVNAPQCGYCQPGQLMQAAALLAQTPKPTDE 121 Query: 132 EVRDGLHGNICRCTGYQNIVKAV 154 ++ + GNICRC YQ I A+ Sbjct: 122 QITSAMAGNICRCGAYQRIKAAI 144 Lambda K H 0.322 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 87 Number of extensions: 2 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 163 Length of database: 152 Length adjustment: 17 Effective length of query: 146 Effective length of database: 135 Effective search space: 19710 Effective search space used: 19710 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 43 (21.2 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory