GapMind for catabolism of small carbon sources

 

Alignments for a candidate for pta in Methylocapsa aurea KYG

Align Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized)
to candidate WP_036261521.1 DL86_RS11540 bifunctional enoyl-CoA hydratase/phosphate acetyltransferase

Query= curated2:Q9X448
         (316 letters)



>NCBI__GCF_000746085.1:WP_036261521.1
          Length = 469

 Score =  306 bits (784), Expect = 6e-88
 Identities = 168/296 (56%), Positives = 204/296 (68%)

Query: 10  SKYDRLIAAARAEAPAVTIVAHPCDETSLGGAIEAAEMGLITPILVAPEAKIRNVAAEHR 69
           S+ +RL+ A R   P  T V  P +E +L GA+  A   LI PIL+   A+IR +AA + 
Sbjct: 154 SQVERLLGACRNLPPMPTAVVAPEEENALLGALAGARENLIIPILIGDAAQIRAIAASNG 213

Query: 70  LDLGRREIVDVPHSHAAAAKAVALIREGRGELLMKGSLHTDELMHEVAASATGLRTQRRI 129
            DL    I D P   AAAA+AV ++ +G    +MKG LHTD L+  V  S  GLR  RRI
Sbjct: 214 ADLAGIAIEDAPSHDAAAARAVEMVHQGLATAVMKGHLHTDALLKHVVKSQGGLRIGRRI 273

Query: 130 SHVFVMDVPGHTDTLFITDAAINIFPDLEAKRDIVQNAIDLWVAIGLGEPRVAILSAVET 189
           SHVFVMD P     L +TDAAINI P LE K DIVQNAIDL  A+G+  P+V +LSAVET
Sbjct: 274 SHVFVMDAPSLHQLLLVTDAAINIAPTLEEKVDIVQNAIDLAQALGIARPKVGVLSAVET 333

Query: 190 VTAKIPSTIEAAALCKMAERGQITGGVLEGPLAFDNAIDQEAARIKGINSPVAGHAQILV 249
           V  KI ST++AAAL KM+ERGQI GGV++GPLA DNAI   AA+ KG+ S VAG A ILV
Sbjct: 334 VNPKIQSTLDAAALSKMSERGQIRGGVVDGPLAMDNAISLSAAKTKGLTSLVAGRADILV 393

Query: 250 VPDLEAGNMLAKNLTFLTHADAAGLVLGARVPIVLTSRADSVRTRLASCAVAALYA 305
            P+LEAGN+LAK LT+   A+ AGLVLGA+VP++LTSRAD  R+RL SCAVA LYA
Sbjct: 394 APNLEAGNILAKELTYAAQAEGAGLVLGAKVPVLLTSRADDERSRLFSCAVAVLYA 449


Lambda     K      H
   0.320    0.133    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 325
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 316
Length of database: 469
Length adjustment: 30
Effective length of query: 286
Effective length of database: 439
Effective search space:   125554
Effective search space used:   125554
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory