GapMind for catabolism of small carbon sources

 

Alignments for a candidate for dopDH in Methylocapsa aurea KYG

Align Alpha-ketoglutaric semialdehyde dehydrogenase; alphaKGSA dehydrogenase; 2,5-dioxovalerate dehydrogenase; EC 1.2.1.26 (characterized)
to candidate WP_036262823.1 DL86_RS14335 aldehyde dehydrogenase family protein

Query= SwissProt::P42236
         (488 letters)



>NCBI__GCF_000746085.1:WP_036262823.1
          Length = 506

 Score =  281 bits (719), Expect = 4e-80
 Identities = 184/485 (37%), Positives = 264/485 (54%), Gaps = 15/485 (3%)

Query: 10  YLNFINGEWVKSQSGDMVKVENPADVNDIVGYVQNSTAEDVERAVTAANEAKTAWRKLTG 69
           Y NFI G+WV +  G   +  +P D   ++  V  S+AEDVE A+ AA+ A+ AW + + 
Sbjct: 19  YDNFIGGKWVPAVKGQTFENISPID-GRVICTVARSSAEDVELALDAAHAARGAWGRTSP 77

Query: 70  AERGQYLYKTADIMEQRLEEIAACATREMGKTLPEAKG-ETARGIAILRYYAGEGMRKTG 128
           AER   L + AD +EQ+L+ +A   T + GK + E K  +    +   RY+AG  +R   
Sbjct: 78  AERSLALLRIADRIEQKLDMLAMVETIDNGKPIRETKAADLPLAVDHFRYFAG-CLRAQE 136

Query: 129 DVIPSTDKDALMFTTRVPLGVVGVISPWNFPVAIPIWKMAPALVYGNTVVIKPATETAVT 188
             I   D D + +    PLGVV  I PWNFP+ + +WK+APAL  GN V++KPA +T ++
Sbjct: 137 GGISEIDHDTVAYHFHEPLGVVAQIIPWNFPLLMAVWKLAPALAAGNCVILKPAEQTPMS 196

Query: 189 CAKIIACFEEAGLPAGVINLVTGPGSVVGQGLAEHDGVNAVTFTGSNQVGKIIGQAALAR 248
              ++    +  LP GV+N++ G G   G+ LA++  +  V FTG    G++I Q A   
Sbjct: 197 VMVLVDMIGDL-LPPGVLNVINGFGVEAGKPLAQNKRIAKVAFTGETTTGRLIMQYASEN 255

Query: 249 GAKYQLEMGGKNPVI----VADDAD--LEAAAEAVITGAFRSTGQKCTATSRVIVQSGIY 302
                LE+GGK+P I    VAD+ D   + A E     A    G+ CT  SR +VQ  IY
Sbjct: 256 IIPVTLELGGKSPNIFFADVADEDDDFFDKALEGFSMFALNQ-GEVCTCPSRALVQEKIY 314

Query: 303 ERFKEKLLQRTKDITIGDSLKEDVWMGPIASKNQLDNCLSYIEKGKQEGASLLIGGEKLE 362
           +RF E+ + R K I  G+ L     +G  AS +QL+  LSY++ G+QEGA +L GG + +
Sbjct: 315 DRFMERAVARVKKIKQGNPLDPATMIGAQASNDQLEKILSYLDIGRQEGAKVLAGGGRAD 374

Query: 363 NG-KYQNGYYVQPAIFDNVTSEMTIAQEEIFGPVIALIKVDSIEEALNIANDVKFGLSAS 421
            G +   G+YVQP I +   ++M I QEEIFGPV+++      EEAL IAND  +GL A 
Sbjct: 375 VGPELAGGFYVQPTILEG-HNKMRIFQEEIFGPVLSVTSFKDDEEALEIANDTLYGLGAG 433

Query: 422 IFTENIGRMLSFIDEIDAGLVRINAESAGVELQAPFGGMKQSSSHSREQGEAAKDFFTAI 481
           ++T N  R   F   I+AG V  N         A FGG KQS    RE  +   D +   
Sbjct: 434 VWTRNGTRAYRFGRAIEAGRVWTNCYHL-YPAHAAFGGYKQSGI-GRENHKMMLDHYQKT 491

Query: 482 KTVFV 486
           K + V
Sbjct: 492 KNMLV 496


Lambda     K      H
   0.315    0.132    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 541
Number of extensions: 21
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 488
Length of database: 506
Length adjustment: 34
Effective length of query: 454
Effective length of database: 472
Effective search space:   214288
Effective search space used:   214288
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory