Alignments for a candidate for pgmA in Clostridium tyrobutyricum FAM22553

GapMind for catabolism of small carbon sources

Alignments for a candidate for pgmA in Clostridium tyrobutyricum FAM22553

Align Phosphoglucomutase; PGM; Alpha-phosphoglucomutase; Glucose phosphomutase; EC 5.4.2.2 (characterized)
to candidate WP_017752866.1 PN53_RS06475 phospho-sugar mutase

Query= SwissProt::P18159
         (581 letters)



>NCBI__GCF_000816635.1:WP_017752866.1
          Length = 577

 Score =  551 bits (1419), Expect = e-161
 Identities = 278/577 (48%), Positives = 388/577 (67%), Gaps = 8/577 (1%)

Query: 3   WRKSYERWKQTEHLDLELKERLIELEGDEQALEDCFYKDLEFGTGGMRGEIGAGTNRMNI 62
           +R+ YE+W  +E +D + K+ L  ++ DEQ +ED FYKDLEFGTGG+RG + AGTNRMNI
Sbjct: 2   YREKYEQWLNSEFIDEDTKKELRNIK-DEQEIEDRFYKDLEFGTGGLRGVMAAGTNRMNI 60

Query: 63  YTVRKASAGFAAYISKQGEEAKKRGVVIAYDSRHKSPEFAMEAAKTLATQGIQTYVFDEL 122
           YT+ K + G + Y+  + + A    V I YDSR  S EFA  AA  L   GI+  +F+ L
Sbjct: 61  YTIGKTTQGLSDYLLNKYKNADI-SVSIGYDSRIMSKEFAERAALNLCANGIKVNLFESL 119

Query: 123 RPTPELSFAVRQLNAYGGIVVTASHNPPEYNGYKVYGDDGGQLPPKEADIVIEQVNAIEN 182
           RPTP LS+AVR   +  GIV+TASHNP +YNGYKVYG+DGGQ+    A  ++  +  +++
Sbjct: 120 RPTPMLSYAVRYFKSKAGIVITASHNPKQYNGYKVYGEDGGQVTDTLAKEILYSIENVDD 179

Query: 183 ELTITVDEENKLKEKGLIKIIGEDIDKVYTEKLTSISVHPEL--SEEVDVKVVFTPLHGT 240
              I     +  + K L+ I+GE++D+ Y EK+  + +  EL  S+  ++ +++TP+HG+
Sbjct: 180 ISKIKNMSRSGAENKKLLNIVGEEVDRSYLEKVKGLGIRKELVKSKAKELNIIYTPIHGS 239

Query: 241 ANKPVRRGLEALGYKNVTVVKEQELPDSNFSTVTSPNPEEHAAFEYAIKLGEEQNADILI 300
            N PVRR L  LGY+NV VVKEQE PD NF T   PNPE+   FE A+ + ++   DI+ 
Sbjct: 240 GNVPVRRALSELGYENVFVVKEQEKPDGNFPTAPYPNPEDPKVFEIALDMAKKIKPDIIF 299

Query: 301 ATDPDADRLGIAVKNDQGKYTVLTGNQTGALLLHYLLSEKKKQGILPDNGVVLKTIVTSE 360
            TDPD DR+G+ VKN  G+Y +LTGNQTG LL +Y+LS  K+   +P+NG ++KTIVT+E
Sbjct: 300 GTDPDCDRIGVIVKNSNGEYKILTGNQTGILLTNYILSSLKEFNSIPENGAIVKTIVTTE 359

Query: 361 IGRAVASSFGLDTIDTLTGFKFIGEKIKEYEASGQYTFQFGYEESYGYLIGDFARDKDAI 420
               +A  +G++ ID LTGFK+IGEKIKE++ SG   F FG+EESYGYL GDF RDKDA+
Sbjct: 360 AASKIAEDYGVEIIDVLTGFKYIGEKIKEFKKSGDKNFIFGFEESYGYLAGDFVRDKDAV 419

Query: 421 QAALLAVEVCAFYKKQGMSLYEALINLFNEYGFYREGLKSLTLKGKQGAEQIEAILASFR 480
            A+ L  E+  +YK +GMSLY+ALI L+++YG+YRE L S+ LKGK+G E+IE I+   R
Sbjct: 420 IASTLICEMSLYYKSKGMSLYDALIALYDKYGYYREKLVSVELKGKEGHEKIEKIMQYLR 479

Query: 481 QNPPQKMAGKQVVTAEDYAVSKRTLLTESKEEAIDLPKSNVLKYFLEDGSWFCLRPSGTE 540
            +    +   ++V   DY +S    L   KE  I+LPKSNVLK+ LED S F +RPSGTE
Sbjct: 480 HSMKNIVGSVKIVKKMDYKLSVEKDLVSIKEIPINLPKSNVLKFILEDKSCFVVRPSGTE 539

Query: 541 PKVKFYFAVKGSSLEDSEKRLAVLSEDVMKTVDEIVE 577
           PK+K Y +V G++L+DS+ R+    E+  K +D I +
Sbjct: 540 PKIKIYLSVIGNTLDDSKNRI----ENFQKKIDNIFD 572


Lambda     K      H
   0.313    0.133    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 883
Number of extensions: 35
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 581
Length of database: 577
Length adjustment: 36
Effective length of query: 545
Effective length of database: 541
Effective search space:   294845
Effective search space used:   294845
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources