Alignments for a candidate for malEIICBA in Clostridium tyrobutyricum FAM22553

GapMind for catabolism of small carbon sources

Alignments for a candidate for malEIICBA in Clostridium tyrobutyricum FAM22553

Align The maltose/maltotriose porter, MalT (31% identical to 4.A.1.1.9) (characterized)
to candidate WP_039653499.1 PN53_RS12165 glucose PTS transporter subunit IIA

Query= TCDB::Q8DS05
         (729 letters)



>NCBI__GCF_000816635.1:WP_039653499.1
          Length = 667

 Score =  327 bits (837), Expect = 2e-93
 Identities = 232/722 (32%), Positives = 360/722 (49%), Gaps = 76/722 (10%)

Query: 10  FEFWQKFGKCLMVVIAVMPAAGLMVSIGNSLALIDPKSTLLVTVANIIAQIGWGVINNLH 69
           F   QK GK LM+ ++V+PAAG+++ +G    L  P           I   G  +  NL 
Sbjct: 6   FSVLQKIGKSLMLPVSVLPAAGILLRLGQPDLLNMP----------YIEAAGNAIFTNLP 55

Query: 70  ILFAVAIGGSWAKERAGGAFAAALAFILINLITGNFYGISLE----MIADKTSYVHNIFG 125
           ++FAV +   ++    GG   AALA ++  LI  N   ++       +A  T+  H++  
Sbjct: 56  MIFAVGVAIGFS----GGEAVAALAAVVGELILENIEKLASSNAATALAQTTAASHHMTL 111

Query: 126 GKMHVADYFINVLGQPALNMGVFVGIISGFVGATAYNKYYNFRKLPDVLSFFNGKRFVPF 185
                   + N++ +  +NMGVF GII G V A  YN++++  KLP VL FF GKRFVP 
Sbjct: 112 KAFMETQVYQNIVTKTTINMGVFGGIIIGIVAALLYNRFHSI-KLPQVLGFFGGKRFVPI 170

Query: 186 VVIVRSTIVALILSVFWPIVQSGINGFGMWIASSQHTAPFLAPFLYGTLERLLLPFGLHH 245
           V    + I+  I    W  VQ  I+      ++S      L P  Y   +RLL+P GLHH
Sbjct: 171 VTSAAALIIGAIGVSIWVPVQGWIDTMANLASNSA-----LGPAFYAAGKRLLIPVGLHH 225

Query: 246 MLTIPMNYTQLGGTYVVLTGAQAGKHVLGQDPLWLAWVQDLIHLKGAGHMSQYHHLLTSV 305
           +   P+   Q G             H +     ++            G   +Y H     
Sbjct: 226 IY-YPVFLYQFG-------------HFISNGITYI------------GDSPRYFH--GDP 257

Query: 306 TPARFKVGQMIGSSGILMGLTLAMYRNVDPDKKEKYKGMFLSAAVAVFLTGVTEPLEYMF 365
           T   F   +       L G  LAM       K+++  GM +S+A   F+TG+TEP+E+ F
Sbjct: 258 TAGIFMASEFPILMFGLPGAALAMIAAAKKSKRKQMAGMMISSAFVAFVTGITEPIEFSF 317

Query: 366 MFAALPLYLVYAVVQGLAFASADLIHLRV---HSFGNIEFLTRTPMAIKAGLAMDIVNFI 422
           +F A  L++ + +V   +      +H+R+    S   I+++     A    L        
Sbjct: 318 IFVAPILFVFHVLVAFCSGLVTSFLHIRLGYTFSASFIDYILGFRYAEHPWLIWP----- 372

Query: 423 VVSVVFGVAMYFITNFMIKKFNLATSGRNGNYDTGDDASDETASNSNAGTANANSQIVKI 482
            V V F +  + +  F+I+  NL T GR       D+  +E    +  G+A A     K+
Sbjct: 373 -VGVAFFLLYFVVFYFLIRAMNLKTPGRE------DEDGEEIVHINVKGSAKA----AKV 421

Query: 483 INLLGGKENISDVDACMTRLRITVTDVAKVGDEAAWKKAGAMGLIVKGNGVQAVYGPKAD 542
           +  +GGK+NI  +DAC+TRLR+T+ D A   DE   +  GA G++  GN VQ V+G +A+
Sbjct: 422 LEAIGGKDNIKVLDACITRLRLTLNDPAV--DEKTLRALGAAGIMKAGNSVQVVFGTEAE 479

Query: 543 VLKSDIQDLLDSGVDIPKTDVTALEEDKTADVSFKGVT--EEIATVADGQVLPITQVHDP 600
            +K DI+ ++ +G  + +++ T   ED +     K V+    + + ADG+++ + +V DP
Sbjct: 480 RIKDDIKAIIANGGVVEESE-TQQGEDTSGGSESKAVSGVNLLLSPADGEIVTLEEVPDP 538

Query: 601 VFSQKMMGDGFAVEPENGNIYSPVAGLVTSVFPTKHALGLLTDDGLEVLVHVGLDTVALN 660
            FS+K++GDGFAV P   NIY+P  G +T +FPTKHA  + T  GLE+L+H+G+DTVALN
Sbjct: 539 TFSEKLLGDGFAVIPSGDNIYAPADGEITVLFPTKHAFAITTAQGLELLIHIGIDTVALN 598

Query: 661 GAPFSAKVKDGQRVALGDLLLVADLEAIKSADRETTVIVAFTNTAELKSVTLEKTGQQAA 720
           G  F+A VK G +V  GDL+L  D + IKS  +     V  TN   + ++ ++K     A
Sbjct: 599 GEGFTAHVKQGDKVKKGDLILNLDSKFIKSKGKNMITPVIVTNMDIVDNIDIKKGKVDHA 658

Query: 721 KT 722
           KT
Sbjct: 659 KT 660


Lambda     K      H
   0.322    0.138    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1028
Number of extensions: 50
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 729
Length of database: 667
Length adjustment: 39
Effective length of query: 690
Effective length of database: 628
Effective search space:   433320
Effective search space used:   433320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources