GapMind for catabolism of small carbon sources

 

propionate catabolism in Oleispira antarctica RB-8

Best path

putP, prpE, pccA, pccB, epi, mcm-large, mcm-small

Rules

Overview: Propionate degradation in GapMind is based on MetaCyc pathways for the 2-methylcitrate cycle (link, link) and for propanoyl-CoA degradation (link, link).

24 steps (15 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
putP propionate transporter; proline:Na+ symporter OLEAN_RS17495
prpE propionyl-CoA synthetase OLEAN_RS11725 OLEAN_RS19275
pccA propionyl-CoA carboxylase, alpha subunit OLEAN_RS02940 OLEAN_RS07665
pccB propionyl-CoA carboxylase, beta subunit OLEAN_RS02925 OLEAN_RS05310
epi methylmalonyl-CoA epimerase OLEAN_RS05320
mcm-large methylmalonyl-CoA mutase, large (catalytic) subunit OLEAN_RS05325
mcm-small methylmalonyl-CoA mutase, small (adenosylcobamide-binding) subunit OLEAN_RS05325 OLEAN_RS10290
Alternative steps:
acn (2R,3S)-2-methylcitrate dehydratase OLEAN_RS05010 OLEAN_RS11565
acnD 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) OLEAN_RS05010
dddA 3-hydroxypropionate dehydrogenase OLEAN_RS17440 OLEAN_RS18030
hpcD 3-hydroxypropionyl-CoA dehydratase OLEAN_RS07250 OLEAN_RS10755
iolA malonate semialdehyde dehydrogenase (CoA-acylating) OLEAN_RS18035
lctP propionate permease
mcmA methylmalonyl-CoA mutase, fused catalytic and adenosylcobamide-binding components OLEAN_RS05325
mctC propionate:H+ symporter
mctP propionate permease
pccA1 propionyl-CoA carboxylase, biotin carboxyl carrier subunit OLEAN_RS07665 OLEAN_RS02940
pccA2 propionyl-CoA carboxylase, biotin carboxylase subunit
pco propanyl-CoA oxidase
prpB 2-methylisocitrate lyase
prpC 2-methylcitrate synthase OLEAN_RS08510
prpD 2-methylcitrate dehydratase
prpF methylaconitate isomerase
SLC5A8 sodium-coupled monocarboxylate transporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory