Alignments for a candidate for ytfR in Nitriliruptor alkaliphilus DSM 45188

GapMind for catabolism of small carbon sources

Alignments for a candidate for ytfR in Nitriliruptor alkaliphilus DSM 45188

Align galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) (characterized)
to candidate WP_052664361.1 NITAL_RS01685 sugar ABC transporter ATP-binding protein

Query= ecocyc::YTFR-MONOMER
         (500 letters)



>NCBI__GCF_000969705.1:WP_052664361.1
          Length = 494

 Score =  322 bits (824), Expect = 2e-92
 Identities = 185/485 (38%), Positives = 283/485 (58%), Gaps = 12/485 (2%)

Query: 3   TDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGENGAGKSTLIKALTGVYHAD 62
           TD    +L    +++ F GV+AL  VD + RRG + ALLGENGAGKSTL++  +G   AD
Sbjct: 2   TDAPDVVLEVRQVTRSFSGVRALRGVDTAFRRGRVHALLGENGAGKSTLVRVCSGAVEAD 61

Query: 63  RGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVADNLFIGREPKR--FGLLRRK 120
            G +WL+G  +   +   A Q GI  V QE  L   +SV  N+F+ R   R   G LR  
Sbjct: 62  GGELWLDGGVVRFASPFVAAQEGIAVVNQEPALAEQLSVLKNIFLPRLSLRRPLGRLRSD 121

Query: 121 EMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAIDLSAKVLILDEPTASLDTQE 180
           E+     +L+A  G S+D+  P++  +VA +Q+V + +A+    ++L +DEP ++L + E
Sbjct: 122 ELRDEGEQLLAELGASIDLDAPVSSLTVAERQLVELAKALATRPRLLFMDEPNSALTSGE 181

Query: 181 VELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVLRNGSFVGCRETCELPQIELVKM 240
            E +  L+ +LR++GV+++ VTH L + +QV+D +TVLR+G  V      E    E++++
Sbjct: 182 TERMLGLIGRLREQGVAVVLVTHRLREAFQVADDVTVLRDGQTVLRAPAAETSIDEVIRI 241

Query: 241 MLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTIAPFDLEVRPGEIVGLAGLLGSGRT 300
           M G +   H  Q     L + K     +    +G  A  +LE+R  EIVGLAGL+G+GR+
Sbjct: 242 MAG-DAPLHEHQ-GPPDLPAGKVALKAEGLTVRGRFADVNLELRESEIVGLAGLVGAGRS 299

Query: 301 ETAEVIFGIKPADSGTALIKGKPQNLRSPHQASVLGIGFCPEDRKTDGIIAAASVRENII 360
           + AE +FG++  D+G   + G+      P  A   GI   PEDRK   + A  S+  N+ 
Sbjct: 300 DLAETLFGLRKPDAGRLYVNGQAVTWSGPSDAMRAGIALVPEDRKVQALFAGLSIDWNLR 359

Query: 361 LALQAQRGWLRPISRKEQQEIAERFIRQLGIRTPSTEQPIEFLSGGNQQKVLLSRWLLTR 420
            A  A       I R ++  IAE  +R+  ++  S   PI  LSGGNQQK +++RW+   
Sbjct: 360 AASVA-------IGRDDRHRIAEA-VRKFRVKFDSLRAPISTLSGGNQQKAVIARWMALG 411

Query: 421 PQFLILDEPTRGIDVGAHAEIIRLIETLCADGLALLVISSELEELVGYADRVIIMRDRKQ 480
           P+ L+LDEPTRGIDV A AE+  L+    A+G+A+L+ISSEL+E++  + R+I+MRD + 
Sbjct: 412 PRTLLLDEPTRGIDVAAKAEVHELVRRTAAEGVAILLISSELDEVLDLSHRIIVMRDGRV 471

Query: 481 VAEIP 485
           V E+P
Sbjct: 472 VTELP 476



 Score = 85.5 bits (210), Expect = 4e-21
 Identities = 61/220 (27%), Positives = 102/220 (46%), Gaps = 4/220 (1%)

Query: 280 DLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASVLGIGF 339
           D   R G +  L G  G+G++    V  G   AD G   + G      SP  A+  GI  
Sbjct: 28  DTAFRRGRVHALLGENGAGKSTLVRVCSGAVEADGGELWLDGGVVRFASPFVAAQEGIAV 87

Query: 340 CPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIRTPSTEQP 399
             ++     +    SV +NI L   + R  L  +   E ++  E+ + +LG      + P
Sbjct: 88  VNQE---PALAEQLSVLKNIFLPRLSLRRPLGRLRSDELRDEGEQLLAELGASI-DLDAP 143

Query: 400 IEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCADGLALLVIS 459
           +  L+   +Q V L++ L TRP+ L +DEP   +  G    ++ LI  L   G+A+++++
Sbjct: 144 VSSLTVAERQLVELAKALATRPRLLFMDEPNSALTSGETERMLGLIGRLREQGVAVVLVT 203

Query: 460 SELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIA 499
             L E    AD V ++RD + V   P AE S+  ++  +A
Sbjct: 204 HRLREAFQVADDVTVLRDGQTVLRAPAAETSIDEVIRIMA 243


Lambda     K      H
   0.321    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 581
Number of extensions: 36
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 500
Length of database: 494
Length adjustment: 34
Effective length of query: 466
Effective length of database: 460
Effective search space:   214360
Effective search space used:   214360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources