Alignments for a candidate for ald-dh-CoA in Herbaspirillum autotrophicum IAM 14942

GapMind for catabolism of small carbon sources

Alignments for a candidate for ald-dh-CoA in Herbaspirillum autotrophicum IAM 14942

Align Acetaldehyde dehydrogenase; Acetaldehyde dehydrogenase [acetylating]; EC 1.2.1.10 (characterized)
to candidate WP_050465872.1 AKL27_RS26330 acetaldehyde dehydrogenase (acetylating)

Query= SwissProt::Q52060
         (312 letters)



>NCBI__GCF_001189915.1:WP_050465872.1
          Length = 314

 Score =  453 bits (1166), Expect = e-132
 Identities = 228/311 (73%), Positives = 267/311 (85%), Gaps = 2/311 (0%)

Query: 2   NQKLKVAIIGSGNIGTDLMIKVLRNAKYLEMGAMVGIDAASDGLARAQRMGVTTTYAGVE 61
           N K+KVAIIGSGNIGTDLMIKVLRNAK+LEMGA VGID ASDGL RA+R+ V  T  G++
Sbjct: 3   NNKVKVAIIGSGNIGTDLMIKVLRNAKHLEMGAFVGIDPASDGLERARRLNVPVTAEGID 62

Query: 62  GLIKLPEFADIDFVFDATSASAHVQNEALLRQAKPGIRLIDLTPAAIGPYCVPVVNLEEH 121
           GL+K+PEFADI   FDATSA AH  + ALL+Q   G+R+IDLTPAAIGPY +P +NL+E 
Sbjct: 63  GLLKMPEFADIRIAFDATSAGAHAHHNALLQQH--GVRVIDLTPAAIGPYVIPAINLDEQ 120

Query: 122 LGKLNVNMVTCGGQATIPMVAAVSRVAKVHYAEIVASISSKSAGPGTRANIDEFTETTSK 181
           LG  N+NMVTCGGQATIP+VAA+SRV KVHYAEIVASISSKSAGPGTRANIDEFTETTS+
Sbjct: 121 LGASNINMVTCGGQATIPVVAAISRVTKVHYAEIVASISSKSAGPGTRANIDEFTETTSR 180

Query: 182 AIEVIGGAAKGKAIIIMNPAEPPLIMRDTVYVLSAAADQAAVAASVAEMVQAVQAYVPGY 241
           AIEV+GGA +GKAII++NPA+PPLIMRDTV+ L+  ADQ A+  S+ +MV+ V +YVPGY
Sbjct: 181 AIEVLGGATRGKAIIVLNPADPPLIMRDTVFALADPADQQAIEDSILKMVEQVNSYVPGY 240

Query: 242 RLKQQVQFDVIPESAPLNIPGLGRFSGLKTSVFLEVEGAAHYLPAYAGNLDIMTSAALAT 301
           R+KQ+VQFD+  E+  LNIPG+G+ SGLK SVFLEVEGAAHYLPAYAGNLDIMTSAALA 
Sbjct: 241 RIKQKVQFDLFDEANALNIPGIGKKSGLKVSVFLEVEGAAHYLPAYAGNLDIMTSAALAC 300

Query: 302 AERMAQSMLNA 312
           A+RMAQ+ L A
Sbjct: 301 ADRMAQTQLAA 311


Lambda     K      H
   0.317    0.132    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 328
Number of extensions: 4
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 312
Length of database: 314
Length adjustment: 27
Effective length of query: 285
Effective length of database: 287
Effective search space:    81795
Effective search space used:    81795
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_050465872.1 AKL27_RS26330 (acetaldehyde dehydrogenase (acetylating))
to HMM TIGR03215 (acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03215.hmm
# target sequence database:        /tmp/gapView.857078.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03215  [M=285]
Accession:   TIGR03215
Description: ac_ald_DH_ac: acetaldehyde dehydrogenase (acetylating)
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.5e-146  473.1   3.6   1.7e-146  472.9   3.6    1.0  1  NCBI__GCF_001189915.1:WP_050465872.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_001189915.1:WP_050465872.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  472.9   3.6  1.7e-146  1.7e-146       1     284 [.       5     309 ..       5     310 .. 0.99

  Alignments for each domain:
  == domain 1  score: 472.9 bits;  conditional E-value: 1.7e-146
                             TIGR03215   1 kvkvaiiGsGnigtdllikllr.sevlelallvGidpesdGlararelgvetsaeGvdalleee...didivf 69 
                                           kvkvaiiGsGnigtdl+ik+lr +++le+ ++vGidp+sdGl+rar+l+v ++aeG+d+ll+++   di+i+f
  NCBI__GCF_001189915.1:WP_050465872.1   5 KVKVAIIGSGNIGTDLMIKVLRnAKHLEMGAFVGIDPASDGLERARRLNVPVTAEGIDGLLKMPefaDIRIAF 77 
                                           79********************99***************************************99999***** PP

                             TIGR03215  70 datsakahaenaklleelgkividltPaavGpyvvPavnleevldaknvnlvtCgGqatiPivaavsrvakvk 142
                                           datsa aha++++ll+++g++vidltPaa+Gpyv+Pa+nl+e+l a+n+n+vtCgGqatiP+vaa+srv+kv+
  NCBI__GCF_001189915.1:WP_050465872.1  78 DATSAGAHAHHNALLQQHGVRVIDLTPAAIGPYVIPAINLDEQLGASNINMVTCGGQATIPVVAAISRVTKVH 150
                                           ************************************************************************* PP

                             TIGR03215 143 yaeivasiasksaGpgtranideftettskaleqvgGakkgkaiiilnPaePpllmrdtvyalveeadeeaie 215
                                           yaeivasi+sksaGpgtranideftetts+a+e +gGa++gkaii+lnPa+Ppl+mrdtv+al++ ad++aie
  NCBI__GCF_001189915.1:WP_050465872.1 151 YAEIVASISSKSAGPGTRANIDEFTETTSRAIEVLGGATRGKAIIVLNPADPPLIMRDTVFALADPADQQAIE 223
                                           ************************************************************************* PP

                             TIGR03215 216 asveemveevqkyvpGyrlkqevvld.................gekvsvlleveGagdylPkyaGnldiltaa 271
                                           +s+ +mve+v++yvpGyr+kq+v++d                 g kvsv+leveGa++ylP+yaGnldi+t+a
  NCBI__GCF_001189915.1:WP_050465872.1 224 DSILKMVEQVNSYVPGYRIKQKVQFDlfdeanalnipgigkksGLKVSVFLEVEGAAHYLPAYAGNLDIMTSA 296
                                           ************************************************************************* PP

                             TIGR03215 272 alavaeklaeell 284
                                           ala+a+++a+++l
  NCBI__GCF_001189915.1:WP_050465872.1 297 ALACADRMAQTQL 309
                                           *********9876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (285 nodes)
Target sequences:                          1  (314 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 19.70
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources