Alignments for a candidate for adh in Herbaspirillum autotrophicum IAM 14942

GapMind for catabolism of small carbon sources

Alignments for a candidate for adh in Herbaspirillum autotrophicum IAM 14942

Align Aldehyde dehydrogenase; Acetaldehyde dehydrogenase; EC 1.2.1.3 (characterized)
to candidate WP_050465302.1 AKL27_RS23455 aldehyde dehydrogenase

Query= SwissProt::A1B4L2
         (508 letters)



>NCBI__GCF_001189915.1:WP_050465302.1
          Length = 506

 Score =  808 bits (2087), Expect = 0.0
 Identities = 388/493 (78%), Positives = 437/493 (88%)

Query: 16  PFEERYDNFIGGEWVAPVSGRYFTNTTPITGAEIGQIARSEAGDIELALDAAHAAKEKWG 75
           PF++RYDN+IGG++V PV+G YF N TP+TG    +IARS A DIELALDAAHAAK  WG
Sbjct: 14  PFKQRYDNYIGGKFVPPVNGAYFENITPVTGKAFCEIARSTAEDIELALDAAHAAKASWG 73

Query: 76  ATSPAERANIMLKIADRMERNLELLATAETWDNGKPIRETMAADLPLAIDHFRYFAGVLR 135
            TS A+RANI+ +IADRME NLELLATAET DNGKPIRETMAAD+PLAIDHFRYFAG +R
Sbjct: 74  KTSLADRANILNRIADRMEANLELLATAETIDNGKPIRETMAADIPLAIDHFRYFAGCIR 133

Query: 136 AQEGSISQIDDDTVAYHFHEPLGVVGQIIPWNFPLLMACWKLAPAIAAGNCVVLKPAEQT 195
           AQEGS++QID +T AYHFHEPLGVVGQIIPWNFP+LM  WK+APA+AAGNC+VLKPAEQT
Sbjct: 134 AQEGSVAQIDSETYAYHFHEPLGVVGQIIPWNFPILMGVWKMAPALAAGNCIVLKPAEQT 193

Query: 196 PAGIMVWANLIGDLLPPGVLNIVNGFGLEAGKPLASSNRIAKIAFTGETTTGRLIMQYAS 255
           PA I+V   LIGDLLPPGVLN+VNGFGLEAGKPLASS RIAKIAFTGET TGRLIMQYAS
Sbjct: 194 PASILVLMELIGDLLPPGVLNVVNGFGLEAGKPLASSKRIAKIAFTGETGTGRLIMQYAS 253

Query: 256 ENLIPVTLELGGKSPNIFFADVAREDDDFFDKALEGFTMFALNQGEVCTCPSRVLIQESI 315
           +NLIPVTLELGGKSPNIFFADV  +DD FFDK LEGF MFALNQGEVCTCPSRVLIQESI
Sbjct: 254 QNLIPVTLELGGKSPNIFFADVMDQDDAFFDKCLEGFAMFALNQGEVCTCPSRVLIQESI 313

Query: 316 YDKFMERAVQRVQAIKQGDPRESDTMIGAQASSEQKEKILSYLDIGKKEGAEVLTGGKAA 375
           YD+F+ERA++RV AIKQG+P +  TMIGAQAS EQ EKILSY+DIG++EGA+ L GG   
Sbjct: 314 YDRFIERAIKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYMDIGRQEGAQTLLGGARQ 373

Query: 376 DLGGELSGGYYIEPTIFRGNNKMRIFQEEIFGPVVSVTTFKDQAEALEIANDTLYGLGAG 435
            L G+L  GYY++PTIF+G+NKMRIFQEEIFGPVVSVTTFKD+AEALEIANDTLYGLGAG
Sbjct: 374 TLNGDLESGYYVQPTIFKGHNKMRIFQEEIFGPVVSVTTFKDEAEALEIANDTLYGLGAG 433

Query: 436 VWSRDANTCYRMGRGIKAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKN 495
           +W+RD +  +R+GR I+AGRVWTNCYH YPAHAAFGGYKQSGIGRE HKMMLDHYQQTKN
Sbjct: 434 LWTRDGSRAFRVGRAIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKN 493

Query: 496 MLVSYSPKKLGFF 508
           +LVSY+P  +GFF
Sbjct: 494 LLVSYNPNAMGFF 506


Lambda     K      H
   0.319    0.136    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 873
Number of extensions: 27
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 508
Length of database: 506
Length adjustment: 34
Effective length of query: 474
Effective length of database: 472
Effective search space:   223728
Effective search space used:   223728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources