Alignments for a candidate for MFS-glucose in Pseudarthrobacter sulfonivorans Ar51

GapMind for catabolism of small carbon sources

Alignments for a candidate for MFS-glucose in Pseudarthrobacter sulfonivorans Ar51

Align The glucose uptake porter, GluP (characterized)
to candidate WP_058930749.1 AU252_RS11015 sugar porter family MFS transporter

Query= TCDB::Q0SE66
         (483 letters)



>NCBI__GCF_001484605.1:WP_058930749.1
          Length = 479

 Score =  498 bits (1281), Expect = e-145
 Identities = 255/451 (56%), Positives = 324/451 (71%), Gaps = 5/451 (1%)

Query: 26  IAVVAALGGLLFGYDSAVINGAVSAIEGKFQVDSALLGFAIASALLGAAAGAMLAGRIAD 85
           +A+  A+GG LFG+DS+V+NGAV AI+ +F +  A+ GFA+A ALLG AAGA LAG++AD
Sbjct: 19  LALAGAVGGFLFGFDSSVVNGAVDAIKDEFALSEAVTGFAVAIALLGCAAGAFLAGKVAD 78

Query: 86  RYGRLVTMRAAAVMFLLSAIGTGFAANVEMLVTFRIVGGIGVGLASVIAPAYIAEISPAR 145
           RYGR+  M+  A++FL+SAIGTGFA  V  L+ +R+VGG+G+GLASVIAPAYI+EISP +
Sbjct: 79  RYGRIPAMKLGALLFLVSAIGTGFAFGVWDLIFWRLVGGLGIGLASVIAPAYISEISPRQ 138

Query: 146 VRGRLGSLQQLAIVTGIFVSLLVDYALAAIAGGSQEELWFGLEAWRWMFLAMCVPALAYG 205
           VRGRL SLQQLAI TGIF +LL D   A  AGG+ +  W GLEAWRWMFLA  VPA+ YG
Sbjct: 139 VRGRLASLQQLAITTGIFAALLSDALFATTAGGADQAFWLGLEAWRWMFLAAAVPAVVYG 198

Query: 206 LLSLTIPESPRYLIAQGRLGEARGILAVLLGEKGLDLKIESIRSTLARETQPSIR-DLKG 264
            ++ T+PESPR+L+ QG+  EAR +   +   +  D  I  I+  +  +     +  L+G
Sbjct: 199 WIAYTLPESPRFLVFQGKEDEARKVFDSIAPSEDTDRHIREIQDAIEEDKLSGQKGSLRG 258

Query: 265 SAFGLMPIVWIGIGLSVFQQFVGINVIFYYSSVLWQAVGFDEGSSLQITVITSVVNIATT 324
             FGL  +VW+GI LSV QQFVGINVIFYYS+ LW+AVGF E  SL I+V TS+ NI  T
Sbjct: 259 KTFGLQAVVWVGIVLSVLQQFVGINVIFYYSTTLWKAVGFQEKDSLTISVATSITNILVT 318

Query: 325 LIAIAYIDRVGRRPLLIIGSAGMAVTLATMAFIFGTASTTVVDGVTTPQLTGLQGPIALV 384
           L+AIA +DR+GRRP+L+ GS GMAV+L TMA  F  A    V   +   L G  GP+ALV
Sbjct: 319 LVAIALVDRIGRRPILLAGSIGMAVSLGTMALAFSAA----VGSGSEISLPGAWGPVALV 374

Query: 385 AANLFVVAFGMSWGPVVWVLLGEAFPNRIRAAALSLAAGAQWAANWLITVTFPSMKDFSL 444
           AAN+FVV+FG SWGP+VWVLLGE FP+RIRA AL LAA AQW AN+ IT++FP M   SL
Sbjct: 375 AANIFVVSFGASWGPLVWVLLGEIFPSRIRARALGLAAAAQWVANFAITLSFPVMAAASL 434

Query: 445 GISYGFYAVCAVLSLVFVLRWVKETKGVELE 475
            ++Y  YA+ A  S  FV+  V ET G+ LE
Sbjct: 435 PLTYAMYALFAAASFFFVMFKVPETNGMSLE 465


Lambda     K      H
   0.324    0.138    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 680
Number of extensions: 27
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 483
Length of database: 479
Length adjustment: 34
Effective length of query: 449
Effective length of database: 445
Effective search space:   199805
Effective search space used:   199805
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources