Alignments for a candidate for aacS in Pseudarthrobacter sulfonivorans Ar51

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Pseudarthrobacter sulfonivorans Ar51

Align acetoacetate-CoA ligase (EC 6.2.1.16) (characterized)
to candidate WP_058932965.1 AU252_RS14440 acetate--CoA ligase

Query= BRENDA::Q9Z3R3
         (650 letters)



>NCBI__GCF_001484605.1:WP_058932965.1
          Length = 673

 Score =  203 bits (517), Expect = 2e-56
 Identities = 176/622 (28%), Positives = 278/622 (44%), Gaps = 44/622 (7%)

Query: 49  RGAFWTAVWEHCKVIGESGEKALVDGDRMLDARFFPEARLNFAENLLRK---TGSGD--A 103
           R AFW           +   +AL D      A++F    +N A N L +    G GD  A
Sbjct: 64  RPAFWARQARELLTWSKDFGQAL-DWSNPPFAKWFVGGEVNAAYNALDRHVENGLGDRVA 122

Query: 104 LIFRGEDKVSYRLTWDELRALVSRLQQALRAQGIGAGDRVAAMMPNMPETIALMLATASV 163
           + F GE   +   T+ +L   V +   A  + G+G GDRVA  +P +PE +  +LA A +
Sbjct: 123 IYFEGEPGDTRTYTYAQLTEEVKKAANAFESLGVGKGDRVAVYLPMIPEAVITLLACARI 182

Query: 164 GAIWSSCSPDFGEQGVLDRFGQIAPKLFIVCDGYWYNGK----RQDVDSKVRAVAKSLGA 219
           GA+ S     F    +  R      KL +  DG +  GK    +  VD  + A   ++  
Sbjct: 183 GAVHSVVFGGFSADALRSRIEDAEAKLVVTADGTYRRGKPSALKTAVDDALAAHGHTVQN 242

Query: 220 PTVIVP-------YAGDSAALAPTVEGGVTLADFIAGFQAGPLVFERLPFGHPLYILFSS 272
             V+         + G     A TVE   T    +                HPL+IL++S
Sbjct: 243 VVVVKRNGQNVDWHEGRDHWWADTVELASTKHTAVGHDSE-----------HPLFILYTS 291

Query: 273 GTTGVPKCIVHSAGGTLLQHLKEHRFHCGLRDGERLFYFTT-CGWMMWNWLA--SGLAVG 329
           GTTG PK I+H+ GG L Q    H+    L     +F+ T   GW+  +     + L  G
Sbjct: 292 GTTGKPKGILHTTGGYLTQGAYTHKAVFDLHPETDVFWCTADVGWVTGHTYVAYAPLING 351

Query: 330 ATLCLYDGSPFCPDGNVLFDYAAAERFAVFGTSAKYIDAVRKGGFTPARTHDLSSLRLMT 389
           AT  +Y+G+P  P     ++     + ++  T+   I    K G      +DL+SLR++ 
Sbjct: 352 ATQVMYEGTPDSPHQGRWWEIIEKYKVSILYTAPTAIRTFMKWGRDIPDKYDLTSLRVLG 411

Query: 390 STGSPLSPEGFSF---VYEGIKPDV--QLASISGGTDIVSCFVLGNPLKPVWRGEIQGPG 444
           S G  ++PE + +   V  G K  +         G  +++        KP   G  Q P 
Sbjct: 412 SVGESINPEAWMWYRDVIGGNKAPIVDTWWQTETGAQMIAPLPGVTATKP---GSAQTPL 468

Query: 445 LGLAVDVWNDEGKPV-RGEKGELVCTRAFPSMPVMFWNDPDGAKYRAAYFDRFDNVWCHG 503
            G+AVDV +++G+ V  G  G LV    +P+M    W DP+  +++  Y+ RF+ ++  G
Sbjct: 469 PGIAVDVVDEQGESVPNGHGGFLVIREPWPAMLRGIWGDPE--RFKETYWSRFEGMYFAG 526

Query: 504 DFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQMDEVAEALCIGQDWEDDVRVV 563
           D A+    G I + GR D  +N  G R+ T EI + +     VAEA  +G   E   + V
Sbjct: 527 DGAKKDEDGDIWLLGRVDDVMNISGHRLSTTEIESALVSHPSVAEAAVVGAADETTGQAV 586

Query: 564 LFVRLARG--VELTEALTREIKNRIRSGASPRHVPAKIIAVADIPRTKSGKIVELAVRDV 621
           +   + RG  V+  +A   E++N +     P   P  I+ V ++P+T+SGKI+   ++DV
Sbjct: 587 VAFVILRGDAVDAGDATIAELRNHVGKEIGPIAKPKTILVVPELPKTRSGKIMRRLLKDV 646

Query: 622 VHGRPVKNKEALANPEALDLFA 643
             GR V +   LA+   +   A
Sbjct: 647 AEGREVGDSTTLADNTVMQQIA 668


Lambda     K      H
   0.322    0.139    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1256
Number of extensions: 72
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 650
Length of database: 673
Length adjustment: 38
Effective length of query: 612
Effective length of database: 635
Effective search space:   388620
Effective search space used:   388620
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources