Alignments for a candidate for paaH in Pseudarthrobacter sulfonivorans Ar51

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaH in Pseudarthrobacter sulfonivorans Ar51

Align FadB (EC 5.3.3.8; EC 1.1.1.35; EC 5.1.2.3; EC 4.2.1.17; EC 1.1.1.36) (characterized)
to candidate WP_058929311.1 AU252_RS02130 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein

Query= metacyc::G1G01-2277-MONOMER
         (715 letters)



>NCBI__GCF_001484605.1:WP_058929311.1
          Length = 738

 Score =  254 bits (649), Expect = 1e-71
 Identities = 203/667 (30%), Positives = 321/667 (48%), Gaps = 41/667 (6%)

Query: 36  TLNELRQAVDAIRADASVKGVIVR---SGKDVFIV-GADITEFVDNFKLPEAELVAGNLE 91
           TL EL   ++ ++  A+ +G IV    +GK  F+V GAD++  V +    +  L    L 
Sbjct: 61  TLVELGTVLEGLKERAA-RGEIVGVGVTGKPYFLVAGADLSA-VKSLDNRDHGLWMAQL- 117

Query: 92  ANRIFNAFEDLEVPTVAAINGIALGGGLEMCLAADYRVMSTSA-RIGLPEVKLGIYPGFG 150
            + ++    +L VP+ A ING+ALGGGLE+ L + YR +ST A  + LPE  +G+ PG+G
Sbjct: 118 GHDVYATLANLGVPSFAFINGVALGGGLEITLQSTYRTVSTGAGALALPEAFIGLVPGWG 177

Query: 151 GTVRLPRLIGSDNAIEWIAAG--KENRA---EDALKVGAVDAVVAPELLLAGALDLIKRA 205
           G   LPRLIG +NA++ +       NR      A ++G  DA+  P   L  +L      
Sbjct: 178 GVYILPRLIGPENAVKVMIENPLSNNRTLNGPQAFQLGIADALFEPADFLEQSLAWAATV 237

Query: 206 ISGELDYKAKRQPKLEKLKLNAIEQMMAFETAKGFVAGQAGPNYPAPVEAIKSIQKAANF 265
           I+GE+   A+           A     A    + FV  +     PAP + +  ++     
Sbjct: 238 ITGEV-VPARANSVDPADPAVAGRWSAAVAAGRSFVESKTSNASPAPAKVLDILEANRTM 296

Query: 266 GRDKALEVEAAGFAKLAKTSVAESLIGLFLN--DQELKRKAKAHDE-IAHDVKQAAVLGA 322
            + ++  +E    A L +T    + +  FL+   +  KR A A D  +A  V +  V+GA
Sbjct: 297 SQAESAALECETLAGLMQTDEFRATVYAFLDLVQKRSKRPAGAPDRRLARPVTKIGVVGA 356

Query: 323 GIMGGGIAYQSAVK-GTPILMKDIREEAIQLGLN----EASKLLGNRVEKGRLTPAKMAE 377
           G+M   +A   A +   P++M DI +  +  G+     E  KLLG    K R++      
Sbjct: 357 GLMASQLALLFARQLKVPVVMTDIDQARVDKGVGYVHAEVDKLLG----KKRISQDAANR 412

Query: 378 ALNAIRPTLSYGDFANVDIVVEAVVENPKVKQAVLAEVEGQVKDDAILASNTSTISINLL 437
               +  ++S   FA+ D V+EAV E   VK+ V AEVE  V  D ILA+NTS++S+  +
Sbjct: 413 TRALVTGSVSKEAFADADFVIEAVFEELNVKKQVFAEVEAIVSPDCILATNTSSLSVTAM 472

Query: 438 AKALKRPENFVGMHFFNPVHMMPLVEVIRGEKSSDVAVATTVAYAKKMGKNPIVVNDCPG 497
           A+ L  PE  VG HFFNPV +MPL+E++R  K+ D  +AT    AK + K  ++V D P 
Sbjct: 473 AQDLAHPERLVGFHFFNPVAVMPLLEIVRAPKTDDAVLATAFELAKGLKKTAVLVKDAPA 532

Query: 498 FLVNRVLFPYFGGFAKLVSAGVDFVRIDKVMEKFGWPMGPAYLMDVVGIDTGHHGRDVMA 557
           F+VNR+L    G        G      D  +   G PM P  L  +VG+    H ++ + 
Sbjct: 533 FVVNRILLRLMGEVTAAFDEGTPAEVADTSLRPMGLPMTPFTLGAMVGLPVAQHVQESLH 592

Query: 558 EGFPDRMKDERRSAVDALYEANRLGQKNGKGFYAYETDKRGKPKKVFDATVLDVLKPIVF 617
             F DR       AV       +L +   K  +    D     +++ ++T    L  + F
Sbjct: 593 AAFGDRF------AVST--NLQKLIENGVKSLWVPAAD---GSQEIPEST----LSLMSF 637

Query: 618 EQREVTDEDIINWMMVPLCLETVRCLEDGIVETAAEADMGLVYGIGFPPFRGGALRYIDS 677
                T ++++  +   L  E    L++G+V    + D+ ++ G G+P F GG   Y+D 
Sbjct: 638 GTSPSTGDNVLRRVQDALAEEIGLMLDEGVVAGPEDIDLCMILGAGWPMFLGGITPYLDR 697

Query: 678 IGVAEFV 684
           +G +E V
Sbjct: 698 VGASERV 704


Lambda     K      H
   0.319    0.137    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1080
Number of extensions: 64
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 715
Length of database: 738
Length adjustment: 40
Effective length of query: 675
Effective length of database: 698
Effective search space:   471150
Effective search space used:   471150
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources