Alignments for a candidate for rhaT' in Pseudarthrobacter sulfonivorans Ar51

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaT' in Pseudarthrobacter sulfonivorans Ar51

Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate WP_058930145.1 AU252_RS07280 sugar ABC transporter ATP-binding protein

Query= TCDB::Q7BSH4
         (512 letters)



>NCBI__GCF_001484605.1:WP_058930145.1
          Length = 506

 Score =  356 bits (914), Expect = e-102
 Identities = 194/473 (41%), Positives = 304/473 (64%), Gaps = 6/473 (1%)

Query: 20  ILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPNEGEILV 79
           +LE+RG+++ F GV+AL  V + + PG V  ++G+NGAGKSTL+K L+G+++P+ GEI  
Sbjct: 10  LLEVRGLTKRFAGVQALKGVDLQVLPGEVHCVMGQNGAGKSTLIKTLSGVHQPDGGEIRW 69

Query: 80  DGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRTIDWQTMNSRSK 139
           +G+  T     AA+D G+  ++QE  + D L+VAENIFLGH  R+    +  +  N+ ++
Sbjct: 70  EGKQITLPRPTAALDLGIATMYQELDVVDGLSVAENIFLGHE-RSTGGVLHVKKTNAIAR 128

Query: 140 ALLTAL-ESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSRKEIDDLFR 198
            LL  L   ++ P+  +  LS A + +V++ARALS + +++IMDEP+A L   E+ +LFR
Sbjct: 129 TLLKRLGHGSLSPSTEVGTLSAANKQIVSMARALSRDTKLIIMDEPSAILDSGEVSNLFR 188

Query: 199 IVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVS-RKTPQDEIVRMMVGR 257
           +VR L  QG A+++ISH+ +E+ +I D   V  +  R    G+S   T + E++R+M GR
Sbjct: 189 VVRELTAQGIAVVYISHRLEEIRQIGDRISVI-KDGRSTANGLSVTDTQKSELIRLMTGR 247

Query: 258 DVENVFP-KIDVAIGGPV-LEIRNYSHRTEFRDISFTLRKGEILGVYGLIGAGRSELSQS 315
           DV NVFP +  V    PV L++ N      F  +S T+R GEILG  GL+G+ RSE+ ++
Sbjct: 248 DVANVFPERKPVPADAPVVLDVDNLELYGHFEKVSLTVRAGEILGFAGLVGSKRSEILET 307

Query: 316 LFGITKPLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNMTLPSLA 375
           ++G  K  SG++ + G+ +   S   A+ AGI   PEER   GL L  P+F+N+TL +  
Sbjct: 308 IYGARKASSGRVSVNGKALRPGSVTSAVNAGIGLSPEERKSQGLILDEPLFKNVTLSTFE 367

Query: 376 RTSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPKVII 435
           R +R G+L  A E   AR+    L+LR A    P  TLSGGNQQK+++ +WL     V++
Sbjct: 368 RFARMGYLNEAAERNAAREQIAALELRPADPDRPARTLSGGNQQKILLARWLVHGTSVLL 427

Query: 436 LDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEG 488
           LDEPT+G+D+G+++ ++  I +LA  G +II+VSSE+ E++G++D VLV+ +G
Sbjct: 428 LDEPTRGVDVGARSEIYDLIRKLAEAGTAIIVVSSEIEEVLGLADNVLVIDDG 480



 Score =  104 bits (259), Expect = 9e-27
 Identities = 76/244 (31%), Positives = 125/244 (51%), Gaps = 12/244 (4%)

Query: 273 PVLEIRNYSHR----TEFRDISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMV 328
           P+LE+R  + R       + +   +  GE+  V G  GAG+S L ++L G+ +P  G++ 
Sbjct: 9   PLLEVRGLTKRFAGVQALKGVDLQVLPGEVHCVMGQNGAGKSTLIKTLSGVHQPDGGEIR 68

Query: 329 LEGQEITIHSPQDAIRAGIVYVPEERGR-HGLALPMPIFQNMTLPSLARTSRRGFLRAAN 387
            EG++IT+  P  A+  GI  + +E     GL++   IF           S  G L    
Sbjct: 69  WEGKQITLPRPTAALDLGIATMYQELDVVDGLSVAENIFLGH------ERSTGGVLHVKK 122

Query: 388 EFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGS 447
             A+AR   +RL   + + S  VGTLS  N+Q V + + L+   K+II+DEP+  +D G 
Sbjct: 123 TNAIARTLLKRLGHGSLSPSTEVGTLSAANKQIVSMARALSRDTKLIIMDEPSAILDSGE 182

Query: 448 KAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLS-AGIFERAELSPEALVR 506
            + +   + EL A+G++++ +S  L EI  + DR+ V+K+G S A      +     L+R
Sbjct: 183 VSNLFRVVRELTAQGIAVVYISHRLEEIRQIGDRISVIKDGRSTANGLSVTDTQKSELIR 242

Query: 507 AATG 510
             TG
Sbjct: 243 LMTG 246


Lambda     K      H
   0.320    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 663
Number of extensions: 37
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 506
Length adjustment: 34
Effective length of query: 478
Effective length of database: 472
Effective search space:   225616
Effective search space used:   225616
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources