Alignments for a candidate for mglA in Pseudarthrobacter sulfonivorans Ar51

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Pseudarthrobacter sulfonivorans Ar51

Align Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized)
to candidate WP_058930145.1 AU252_RS07280 sugar ABC transporter ATP-binding protein

Query= TCDB::G4FGN3
         (494 letters)



>NCBI__GCF_001484605.1:WP_058930145.1
          Length = 506

 Score =  398 bits (1023), Expect = e-115
 Identities = 209/492 (42%), Positives = 316/492 (64%), Gaps = 4/492 (0%)

Query: 2   KPILEVKSIHKRFPGVHALKGVSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEI 61
           +P+LEV+ + KRF GV ALKGV ++  PGEVH ++G+NGAGKSTL+K ++GV+QPD GEI
Sbjct: 8   RPLLEVRGLTKRFAGVQALKGVDLQVLPGEVHCVMGQNGAGKSTLIKTLSGVHQPDGGEI 67

Query: 62  IYEGRGVRWNHPSEAINAGIVTVFQELSVMDNLSVAENIFMGDEEKRGIFIDYKKMYREA 121
            +EG+ +    P+ A++ GI T++QEL V+D LSVAENIF+G E   G  +  KK    A
Sbjct: 68  RWEGKQITLPRPTAALDLGIATMYQELDVVDGLSVAENIFLGHERSTGGVLHVKKTNAIA 127

Query: 122 EKFMKEEFGIEIDPEEKLGKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQKETEKLF 181
              +K      + P  ++G  S A +Q+V +ARA+ +  K++I+DEP++ L   E   LF
Sbjct: 128 RTLLKRLGHGSLSPSTEVGTLSAANKQIVSMARALSRDTKLIIMDEPSAILDSGEVSNLF 187

Query: 182 EVVKSLKEKGVAIIFISHRLEEIFEICDKVSVLRDGEYIGTD-SIENLTKEKIVEMMVGR 240
            VV+ L  +G+A+++ISHRLEEI +I D++SV++DG       S+ +  K +++ +M GR
Sbjct: 188 RVVRELTAQGIAVVYISHRLEEIRQIGDRISVIKDGRSTANGLSVTDTQKSELIRLMTGR 247

Query: 241 KLEKFYIKEAHEPGE--VVLEVKNLS-GERFENVSFSLRRGEILGFAGLVGAGRTELMET 297
            +   + +    P +  VVL+V NL     FE VS ++R GEILGFAGLVG+ R+E++ET
Sbjct: 248 DVANVFPERKPVPADAPVVLDVDNLELYGHFEKVSLTVRAGEILGFAGLVGSKRSEILET 307

Query: 298 IFGFRPKRGGEIYIEGKRVEINHPLDAIEQGIGLVPEDRKKLGLILIMSIMHNVSLPSLD 357
           I+G R    G + + GK +       A+  GIGL PE+RK  GLIL   +  NV+L + +
Sbjct: 308 IYGARKASSGRVSVNGKALRPGSVTSAVNAGIGLSPEERKSQGLILDEPLFKNVTLSTFE 367

Query: 358 RIKKGPFISFKREKELADWAIKTFDIRPAYPDRKVLYLSGGNQQKVVLAKWLALKPKILI 417
           R  +  +++   E+  A   I   ++RPA PDR    LSGGNQQK++LA+WL     +L+
Sbjct: 368 RFARMGYLNEAAERNAAREQIAALELRPADPDRPARTLSGGNQQKILLARWLVHGTSVLL 427

Query: 418 LDEPTRGIDVGAKAEIYRIMSQLAKEGVGVIMISSELPEVLQMSDRIAVMSFGKLAGIID 477
           LDEPTRG+DVGA++EIY ++ +LA+ G  +I++SSE+ EVL ++D + V+  GK+     
Sbjct: 428 LDEPTRGVDVGARSEIYDLIRKLAEAGTAIIVVSSEIEEVLGLADNVLVIDDGKVLTQTK 487

Query: 478 AKEASQEKVMKL 489
           A +  +  V+ L
Sbjct: 488 ASDIDEHGVLDL 499



 Score = 75.9 bits (185), Expect = 3e-18
 Identities = 53/222 (23%), Positives = 104/222 (46%), Gaps = 7/222 (3%)

Query: 23  VSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEIIYEGRGVRWNHPSEAINAGIV 82
           VS+    GE+    G  G+ +S +++ I G  +   G +   G+ +R    + A+NAGI 
Sbjct: 281 VSLTVRAGEILGFAGLVGSKRSEILETIYGARKASSGRVSVNGKALRPGSVTSAVNAGIG 340

Query: 83  TVFQELSVMDNLSVAENIFMG---DEEKRGIFIDYKKMYREAEKFMKEEFGIEI---DPE 136
              +E      L + E +F        +R   + Y     E     ++   +E+   DP+
Sbjct: 341 LSPEERK-SQGLILDEPLFKNVTLSTFERFARMGYLNEAAERNAAREQIAALELRPADPD 399

Query: 137 EKLGKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQKETEKLFEVVKSLKEKGVAIIF 196
                 S   QQ + +AR +     VL+LDEPT  +      +++++++ L E G AII 
Sbjct: 400 RPARTLSGGNQQKILLARWLVHGTSVLLLDEPTRGVDVGARSEIYDLIRKLAEAGTAIIV 459

Query: 197 ISHRLEEIFEICDKVSVLRDGEYIGTDSIENLTKEKIVEMMV 238
           +S  +EE+  + D V V+ DG+ +      ++ +  ++++++
Sbjct: 460 VSSEIEEVLGLADNVLVIDDGKVLTQTKASDIDEHGVLDLVM 501


Lambda     K      H
   0.318    0.138    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 635
Number of extensions: 31
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 494
Length of database: 506
Length adjustment: 34
Effective length of query: 460
Effective length of database: 472
Effective search space:   217120
Effective search space used:   217120
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources