Alignments for a candidate for paaK in Hyphomicrobium sulfonivorans WDL6

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaK in Hyphomicrobium sulfonivorans WDL6

Align phenylacetate-CoA ligase (EC 6.2.1.30) (characterized)
to candidate WP_245281768.1 APY04_RS00535 long-chain fatty acid--CoA ligase

Query= BRENDA::A7KUK6
         (562 letters)



>NCBI__GCF_001541235.1:WP_245281768.1
          Length = 575

 Score =  201 bits (510), Expect = 8e-56
 Identities = 154/503 (30%), Positives = 245/503 (48%), Gaps = 43/503 (8%)

Query: 68  RKGDVLALFTPNSIDTPVVMWGTLWAGGTISPANPGYTVDELAFQLKNSHAKGLVT---- 123
           RKG  + LF PNS    +  +  L AGGT+   NP YTV EL  Q+++S  + ++T    
Sbjct: 85  RKGTKVGLFLPNSPTFIIYFFAVLKAGGTVVNFNPLYTVAELTHQVEDSDTELMITLDLK 144

Query: 124 ----------QASVLPVAREAAKKVGMPEDRIILIGDQRDPDARVKHFTSVRN------- 166
                     Q+  L  A  A     +P  +  L    R  +      + V N       
Sbjct: 145 VLFDKVEALLQSGCLARALVAPFPSLLPATKAALFRLFRSRELARPLSSPVANRVTLEGE 204

Query: 167 -ISGATRYRKQKITPAKDVAFLVYSSGTTGVPKGVMISHRNIVANIRQQFIAEGEMLSWN 225
            ++GA   +   I P  DVA L Y+ GTTG PKG M++H N+  N++Q      ++    
Sbjct: 205 ALAGAATMQPVAIDPENDVAVLQYTGGTTGTPKGAMLTHANVYINVQQVAATAPDL---- 260

Query: 226 GGPDGKGDRVLAFLPFYHIYGLTCLITQALYKGYHLIVMSKFDIEKWCAHVQNYRCSFSY 285
              +   +RVL  LPF+H++ LT ++   + K   +I+M +F ++     +   + +   
Sbjct: 261 ---EPGVERVLGVLPFFHVFALTVVMNLGIAKAAEIIIMPRFALDDALKLIDRTKPTIMP 317

Query: 286 IVPPVVLLLGKHPVVDKYDLSSLRMMNSGAAPLTQELVEAVYSRIKVGIKQGYGLSETSP 345
            VP +   +  HP +  +DLSSL+   SG A L  E+ +   +     + +GYGLSE SP
Sbjct: 318 GVPTLFNAIMNHPKIASFDLSSLKFCLSGGAALPIEVKQRFEAITGCKVVEGYGLSEASP 377

Query: 346 TTHSQRWEDWREAMGSVGRLMPNMQAKYMTMPEDGSEPKEVGEGEVGELYLKGPNVFLGY 405
                  +    A GS+G  +P +    +++ +  +   EV  GE GE+ +KGP V  GY
Sbjct: 378 VVACNPIDGPARA-GSIG--IP-LVGTTISLRDLNNPELEVPLGEKGEICVKGPQVMKGY 433

Query: 406 HENPEATKGCLSEDGWFQTGDVGYQDAKGNFYITDRVKELIKYKGFQVPPAELEGYLVDN 465
           ++ PE T   +  + + +TGDV   DA G FYI DR+K+LI   GF V P  +E  L ++
Sbjct: 434 YKRPEDTAAQMVGE-YLRTGDVACMDADGFFYIKDRIKDLIISSGFNVYPRRVEEALYEH 492

Query: 466 DAIDDVAVIGIESETHGSEVPMACVVRSAKSKSSGTSEKDEAARIIKWLDSKVASHKRLR 525
            A+ +V VIGI  +  G E P A V    K+ ++ T      A +++ L +++ S   L 
Sbjct: 493 PAVAEVTVIGIRDKKRG-EAPKAFV--RLKTDTTVT-----VAELMEHLQTRI-SRIELP 543

Query: 526 GGVHFVDEIPKNPSGKILRRILK 548
             + F  E+PK   GK+ ++ LK
Sbjct: 544 AEIEFRAELPKTLIGKLSKKELK 566


Lambda     K      H
   0.317    0.136    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 683
Number of extensions: 37
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 562
Length of database: 575
Length adjustment: 36
Effective length of query: 526
Effective length of database: 539
Effective search space:   283514
Effective search space used:   283514
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources