GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hisQ in Hydrogenophaga taeniospiralis CCUG 15921 NBRC 102512

Align Probable permease of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate WP_068168721.1 HTA01S_RS07505 ABC transporter permease subunit

Query= TCDB::Q9HU30
         (231 letters)



>NCBI__GCF_001592305.1:WP_068168721.1
          Length = 226

 Score =  187 bits (474), Expect = 2e-52
 Identities = 102/223 (45%), Positives = 145/223 (65%), Gaps = 4/223 (1%)

Query: 4   DLHGFGEQLLAGTWMTLKLSLAAVCVGLLLGLLGAIAKTSKYAALRFLGGTYTTIVRGVP 63
           DL  +  QLL G W+T++L+L+++ VGL LGL+ A    S    LR      T ++RG+P
Sbjct: 3   DLWLYAGQLLQGAWVTVQLALSSLVVGLALGLVLAAGSLSGRTLLRRAVRLCTGLLRGIP 62

Query: 64  ETLWVLMIYFGTVSGLNALGDLFGKPDLALSPFAAGTLALGLCFGAYATEVFRGALLSIP 123
           E L VL+ YFG  + +N    L G  ++  SPFAAG  AL + F AYA+EVFRG+  +IP
Sbjct: 63  EFLIVLVCYFGLSNLIN--NHLDGAVEI--SPFAAGVFALSIVFAAYASEVFRGSFAAIP 118

Query: 124 RGHREAGQALGLSPGRIFWRIVLPQIWRVALPGLGNLYLILLKDTALVSLITLDEIMRKA 183
           RG  EA QA GL+  + F+ + LPQ WR+ALP LGNL+  LLKDT+LVS++ L+++++K+
Sbjct: 119 RGQIEAAQAFGLTATQTFFAVRLPQAWRIALPSLGNLWQSLLKDTSLVSVVGLEDLLKKS 178

Query: 184 QVASNATKEPFTFYMTAAAIYLSLTVVIMVALHFLERRAGRGF 226
            +A+ AT +PF F++ AA +Y            +LERRAGRGF
Sbjct: 179 NMAAQATHQPFLFFLAAATVYFVFLHASQPVFAWLERRAGRGF 221


Lambda     K      H
   0.327    0.143    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 160
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 231
Length of database: 226
Length adjustment: 23
Effective length of query: 208
Effective length of database: 203
Effective search space:    42224
Effective search space used:    42224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory