Alignments for a candidate for dddA in Hydrogenophaga taeniospiralis CCUG 15921 NBRC 102512

GapMind for catabolism of small carbon sources

Alignments for a candidate for dddA in Hydrogenophaga taeniospiralis CCUG 15921 NBRC 102512

Align 3-hydroxypropionate dehydrogenase (EC 1.1.1.59) (characterized)
to candidate WP_068167459.1 HTA01S_RS04880 choline dehydrogenase

Query= metacyc::MONOMER-15202
         (579 letters)



>NCBI__GCF_001592305.1:WP_068167459.1
          Length = 547

 Score =  353 bits (905), Expect = e-101
 Identities = 224/547 (40%), Positives = 293/547 (53%), Gaps = 29/547 (5%)

Query: 36  FDYIVVGAGTAGCLLANRLSADPANRVLLIEAGGRDNYHWIHIPVGYLYCINNPRTDWRF 95
           +DY++VG G+AGC+LA+RLS DP  RV L+EAG  D    IH P G           W  
Sbjct: 2   YDYLIVGGGSAGCVLASRLSEDPQVRVALLEAGPADKNVLIHCPAGLAVMAKYELNGWGL 61

Query: 96  RTEPDPGLNGRSLIYPRGKTLGGCSSINGMLYLRGQARDYDGWAELTGDDAWRWDNCLPD 155
            T P PGLNGR    PRGK LGG SSIN M+Y RG A DYD WA   G+  W + + LP 
Sbjct: 62  ATVPQPGLNGRRGYQPRGKVLGGSSSINAMIYARGHASDYDAWA-AEGNPGWSFADVLPY 120

Query: 156 FMRHEDHYRLDEGGDADPDHYKFHGHGGEWRIEKQRLKWQVLADFATAAVEAGVPRTRDF 215
           F R E + R   G DA       HG GG   +   R     L  F  A  +AG     DF
Sbjct: 121 FKRAEHNER---GADA------LHGQGGPLNVMDLRSPNPFLPAFMEAGQQAGYRLNNDF 171

Query: 216 NRGDNEGVDAFEVNQRSGWRWNASKAFLRGVEQRGNLTVWH---STQVLKLDFASGEGSE 272
           N  + EG+  ++V  ++G R++A+KA+L     R NL V     +T+VL       E  +
Sbjct: 172 NGPEQEGIGPYQVTHKNGERFSAAKAYLTPHLNRPNLNVITGALTTRVLT------EVVD 225

Query: 273 PRCCGVTVE---RAGKKVVTTARC----EVVLSAGAIGSPQLLQLSGIGPTALLAEHAIP 325
            R C V VE    AG+  + + R     EVVLSAGA GSPQLL LSGIGP A L EH I 
Sbjct: 226 GRPCAVGVEYRPDAGRGALQSLRLKPGGEVVLSAGAFGSPQLLMLSGIGPAAHLREHGIG 285

Query: 326 VVADLPGVGENLQDHLQIRSIYKV-KGAKTLNTMANSLIGKAKIGLEYILKRSGPMSMAP 384
           VV DLPGVG NL DH  +  +    K  +        ++   K   E+  +RSGP++   
Sbjct: 286 VVHDLPGVGANLHDHPDVVMVVNAPKLTELFGLSWTGVVNMVKGLFEWRRQRSGPLTTNF 345

Query: 385 SQLCIFTRSSKEYEHPNLEYHVQPLSLEAFGQPLHDFPAITASVCNLNPTSRGTVRIKSG 444
           ++   F +SS E   P+L+ H     L   G+        +  VC L P SRGT+R+ S 
Sbjct: 346 AEAGGFIKSSPEEPIPDLQLHFVVGKLVDHGRKTVLGHGYSCHVCLLRPRSRGTLRLASA 405

Query: 445 NPRQAPAISPNYLSTEEDRQVAADSLRVTRHIASQPAFAKYDPEEFKPGVQYQSDEDLAR 504
           +P+ AP I P +L   +D        +  R +  QPA A++   E K   + QSD  + +
Sbjct: 406 DPQVAPLIDPAFLQDADDTARLVRGFKCMRELLQQPALARHGGMESKASAEAQSDAQIEQ 465

Query: 505 LAGDIGTTIFHPVGTAKMGRDDDPMAVVDSHLRVRGVTGLRVVDASIMPTITSGNTNSPT 564
              +   TI+HPVGT +MG   D  AVVD+ LRV GV GLRVVDAS+MP++  GNTN+P 
Sbjct: 466 FIRNHADTIYHPVGTCRMG--PDGAAVVDARLRVHGVAGLRVVDASVMPSVVGGNTNAPV 523

Query: 565 LMIAEKA 571
           +M+AEKA
Sbjct: 524 IMMAEKA 530


Lambda     K      H
   0.318    0.135    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 922
Number of extensions: 38
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 579
Length of database: 547
Length adjustment: 36
Effective length of query: 543
Effective length of database: 511
Effective search space:   277473
Effective search space used:   277473
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources