Alignments for a candidate for rhaA in Hydrogenophaga taeniospiralis CCUG 15921 NBRC 102512

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaA in Hydrogenophaga taeniospiralis CCUG 15921 NBRC 102512

Align L-rhamnose isomerase (EC 5.3.1.14) (characterized)
to candidate WP_084236003.1 HTA01S_RS10220 L-rhamnose catabolism isomerase

Query= reanno::Smeli:SMc02321
         (430 letters)



>NCBI__GCF_001592305.1:WP_084236003.1
          Length = 428

 Score =  623 bits (1606), Expect = 0.0
 Identities = 305/426 (71%), Positives = 351/426 (82%), Gaps = 1/426 (0%)

Query: 5   ISTSVLDAENASRRDALTRDYESLGDRLARRGIDIDAVKAKVAAYGVAVPSWGVGTGGTR 64
           I +S L + N S       DYE LG++LARR IDI+ V+ +VAA+GVA+PSWGVGTGGTR
Sbjct: 4   IDSSFLASHNYSLARHAHHDYEHLGEQLARRDIDIETVRQQVAAFGVAIPSWGVGTGGTR 63

Query: 65  FARFPGPGEPRNIFDKLEDCAVIQQLTRATPAVSLHIPWDKVSDLGALKEKGSALGLSFD 124
           FARFPG GEPR++FDKL+DC VIQQL RATP VSLHIPWDK SD G L++  ++ GL+FD
Sbjct: 64  FARFPGQGEPRHVFDKLQDCGVIQQLARATPTVSLHIPWDKCSDWGELRQAAASAGLAFD 123

Query: 125 AMNSNTFSDAPGQAHSYKFGSLSHTDSATRRQAIEHNLECVEIGKALGSKALTVWVGDGS 184
           AMNSNTFSD PGQAHSYK+GSLSHTD+ATR QA+ HNLEC++IG+ALGSKALTVWVGDGS
Sbjct: 124 AMNSNTFSDQPGQAHSYKYGSLSHTDAATRAQAVAHNLECIDIGQALGSKALTVWVGDGS 183

Query: 185 NFPGQSNFTRAFERYLDSMKAVYAALPDDWRIFTEHKMFEPAFYSTVVQDWGTNYLIAQE 244
           NFPGQ +F RAF+RYL+S + +YAALP DWR+  EHK+ EPAFY+TV+QDWG+++L AQ 
Sbjct: 184 NFPGQQHFRRAFDRYLESAQQIYAALPGDWRMMLEHKICEPAFYATVIQDWGSSFLAAQT 243

Query: 245 LGPKAFCLVDLGHHAPNVNIEMIVARLIQFKKLGGFHFNDSKYGDDDLDTGSIDPYRLFL 304
           LGPKA CLVDLGHHAPNVNIE+IVARLI   KL GFHFNDSKYGDDDLD GS+ PYRLFL
Sbjct: 244 LGPKAQCLVDLGHHAPNVNIELIVARLIAAGKLAGFHFNDSKYGDDDLDAGSVSPYRLFL 303

Query: 305 VFNELVDAETRAANGFDPAHMLDQSHNVTDPIESLMTSAMEVGRAYAQALIVDRKALAGY 364
           VFNELV A       FDPA+MLDQSHNVTDPIESLMTSA++V R+YAQAL+VDR AL   
Sbjct: 304 VFNELV-AAAHEGVPFDPAYMLDQSHNVTDPIESLMTSAVQVQRSYAQALLVDRAALDAA 362

Query: 365 QEENDALMASETLKTAFRTDVEPILATARLENDGAIAPVAAYRASGYRARVAAERPAVAG 424
           QE NDAL A+  LK AF+TDV P+L   RL+  GAI PVAAYRASGYR RVA ERPA  G
Sbjct: 363 QEANDALTATHLLKQAFQTDVTPLLQRVRLDAGGAIDPVAAYRASGYRQRVAGERPAAVG 422

Query: 425 GGGGIV 430
           GGGGIV
Sbjct: 423 GGGGIV 428


Lambda     K      H
   0.318    0.134    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 606
Number of extensions: 22
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 428
Length adjustment: 32
Effective length of query: 398
Effective length of database: 396
Effective search space:   157608
Effective search space used:   157608
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources