Alignments for a candidate for treEIIA in Hydrogenophaga taeniospiralis CCUG 15921 NBRC 102512

GapMind for catabolism of small carbon sources

Alignments for a candidate for treEIIA in Hydrogenophaga taeniospiralis CCUG 15921 NBRC 102512

Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate WP_068174654.1 HTA01S_RS20310 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::pseudo3_N2E3:AO353_15995
         (844 letters)



>NCBI__GCF_001592305.1:WP_068174654.1
          Length = 588

 Score =  262 bits (669), Expect = 5e-74
 Identities = 194/581 (33%), Positives = 282/581 (48%), Gaps = 43/581 (7%)

Query: 277 LRGVCASAGSAFGYVVQVAERTLEMPEF--AADQQL-ERESLERA-------LMHATQAL 326
           + G   S G A G  V VA   +++  +   ADQ   E E L +A       ++   Q L
Sbjct: 5   VHGQAVSRGIAIGRAVIVASSRVDVAHYFVTADQTTAEIERLRQARNAVMEEIVRVQQGL 64

Query: 327 QRLRDNAAGEAQADIFKAHQELLEDPSLLEQAQALIAEGK-SAAFAWNSATEATATLFKS 385
             L  N A    + +   H  LL+D  L+   +  I +   +A +A  +  E  A  F  
Sbjct: 65  GELGSNDAHPELSALLDVHLMLLQDEQLISGVKHWIVDRHYNAEWALTTQLEVIARQFDE 124

Query: 386 LGSTLLAERALDLMDVGQRVLKLILGVP-----------------DGVWELPDQAILIAE 428
           +    L ER  DL  V +R+L+ + GV                  D V ++P   +LIA 
Sbjct: 125 MEDPYLRERKADLEQVVERMLRFMRGVASPIMAPVRTEARRDAAHDSVVDVP--LVLIAH 182

Query: 429 QLTPSQTAALDTGKVLGFATVGGGATSHVAILARALGLPAVCGLPLQVLSLASGTRVLLD 488
            L+P+           GF T  GG TSH AI+AR++ +PAV G       +     V++D
Sbjct: 183 DLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARSASHLVEQDDWVIID 242

Query: 489 ADKGELHLDPAVSVIEQLHAKRQQQRQRHQHELENAARAAVTRDGHHFEVTANVASLAET 548
            D G + +DP+  ++ +   K++Q     +         AVT DG   E+ AN+    + 
Sbjct: 243 GDAGVVLVDPSPVLLAEYGFKQRQGEVERERLSRLKNTPAVTLDGQRIELQANIEQPDDA 302

Query: 549 EQAMSLGAEGIGLLRSEFLYQQRS-VAPSHDEQAGTYSAIARALGPQRNL--VVRTLDVG 605
             A+  GA G+GL R+EFL+  R+   P  +EQ   Y A  RA+   + L   +RT+DVG
Sbjct: 303 VAALKAGAVGVGLFRTEFLFMGRNGKLPDEEEQ---YQAYRRAVEGMQGLPVTIRTVDVG 359

Query: 606 GDKPLAYVPM---DSEANPFLGMRGIRLCLERPQLLREQFRAILSSAGLARLHIMLPMVS 662
            DKPL   P+   +   NP LG+R IR  L  P +   Q RAIL +A    +++++PM++
Sbjct: 360 ADKPLDRTPVRAGEDHLNPALGLRAIRWSLADPAMFLAQLRAILRAAAHGSVNLLIPMLA 419

Query: 663 QLSELRLARLMLEEEALALGLRELP----KLGIMIEVPAAALMADLFAPEVDFFSIGTND 718
             SE+R   ++++     L  R       +LG MIE+PAAAL   LF    DF SIGTND
Sbjct: 420 HASEIRQTLVLVDRAREQLNTRGQVYGPVRLGAMIEIPAAALTIPLFLRHFDFLSIGTND 479

Query: 719 LTQYTLAMDRDHPRLASQADSFHPSVLRLIASTVKAAHAHGKWVGVCGALASETLAVPLL 778
           L QYTLA+DR    +A   D  HP+VL+L+AST+    A GK V VCG +A +     LL
Sbjct: 480 LIQYTLAIDRADEAVAHLYDPVHPAVLQLLASTIAQCRAQGKGVSVCGEMAGDVTMTRLL 539

Query: 779 LGLGVDELSVSVPLIPAIKAAIREVELSDCQAIAHQVLGLE 819
           LGLG+   S+    I A+K  I   + +  Q  A  VL  E
Sbjct: 540 LGLGLRTFSMHPSQILAVKQQILRSDTARLQVWAQSVLVAE 580


Lambda     K      H
   0.318    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1029
Number of extensions: 44
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 844
Length of database: 588
Length adjustment: 39
Effective length of query: 805
Effective length of database: 549
Effective search space:   441945
Effective search space used:   441945
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources