Alignments for a candidate for nagEIIA in Alkalihalobacterium alkalinitrilicum DSM 22532

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagEIIA in Alkalihalobacterium alkalinitrilicum DSM 22532

Align PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized)
to candidate WP_078430180.1 BK574_RS22665 glucose-specific PTS transporter subunit IIBC

Query= CharProtDB::CH_001857
         (699 letters)



>NCBI__GCF_002019605.1:WP_078430180.1
          Length = 656

 Score =  736 bits (1900), Expect = 0.0
 Identities = 385/699 (55%), Positives = 496/699 (70%), Gaps = 46/699 (6%)

Query: 1   MFKALFGVLQKIGRALMLPVAILPAAGILLAIGNAMQNKDMIQVLHFLSNDNVQLVAGVM 60
           MFK  FG LQ++GRALM PV++LPAAG+LL +G+                +NV L   +M
Sbjct: 1   MFKKAFGTLQRVGRALMTPVSVLPAAGLLLGLGH----------------ENV-LDIPIM 43

Query: 61  ESAGQIVFDNLPLLFAVGVAIGLANGDGVAGIAAIIGYLVMNVSMSAVLLANGTIPSDSV 120
             AG ++F NL LLFAVGVAIGLA+GDGVAG+AA+IGYLVMN ++  +    G       
Sbjct: 44  AEAGGVIFGNLALLFAVGVAIGLADGDGVAGLAAVIGYLVMNTTLGIMAGYYGA------ 97

Query: 121 ERAKFFTENHPAYVNMLGIPTLATGVFGGIIVGVLAALLFNRFYTIELPQYLGFFAGKRF 180
                        V +LGI TL TGVFGGII+G++AA L+ RFY IELP +LGFFAGKRF
Sbjct: 98  -----------ETVTVLGIETLQTGVFGGIIMGLVAAALYKRFYNIELPPFLGFFAGKRF 146

Query: 181 VPIVTSISALILGLIMLVIWPPIQHGLNAFSTGLVEANPTLAAFIFGVIERSLIPFGLHH 240
           VPI+T+ +A++LG+I + +WPPIQ  ++ FS    E   T+AAF+FG  +R+LIPFGLHH
Sbjct: 147 VPIITAATAVLLGIIFVFVWPPIQGVIDWFSHLATETGTTVAAFVFGFTQRALIPFGLHH 206

Query: 241 IFYSPFWYEFFSYKSAAGEIIRGDQRIFMAQIKDGVQLTAGTFMTGKYPFMMFGLPAAAL 300
           IFY PFW+EF ++ +AAG+++RGD   F A        TAGTFM G +PFM+FGLPAAAL
Sbjct: 207 IFYQPFWFEFGTFTNAAGDVVRGDMTRFFAG-----DPTAGTFMAGLFPFMLFGLPAAAL 261

Query: 301 AIYHEAKPQNKKLVAGIMGSAALTSFLTGITEPLEFSFLFVAPVLFAIHCLFAGLSFMVM 360
           AIYHEA+P+ KK VAGIM SAALTSFLTGITEP+EF+FLFVAPVLF IHC+FAG SF++M
Sbjct: 262 AIYHEARPERKKAVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFLIHCIFAGFSFVIM 321

Query: 361 QLLNVKIGMTFSGGLIDYFLFGILPNRTAWWLVIPVGLGLAVIYYFGFRFAIRKFNLKTP 420
            LL VK G TFSGG IDY L+  L   +  WL+IPVG+    +YYFGFRFAIRK+NLKTP
Sbjct: 322 DLLGVKAGFTFSGGFIDYVLYWGLSTNS--WLIIPVGIAFGFVYYFGFRFAIRKWNLKTP 379

Query: 421 GREDAAEETAAPGKTGEAGDLPYEILQAMGDQENIKHLDACITRLRVTVNDQKKVDKDRL 480
           GRE   E+ +     G   +L   +L A+G ++NI  LDACITRLRV+V +  +VD  +L
Sbjct: 380 GREPKEEQVSEEVAKGGKRELASNVLHALGGKQNITALDACITRLRVSVKEPSEVDSAQL 439

Query: 481 KQLGASGVLEVGNNIQAIFGPRSDGLKTQMQDIIAGRKPRPEPKTSAQEEVGQQVEEVIA 540
           K+LGASGVLE+GNNIQAIFG +SD LK Q++D+I G+    E      ++   + + V  
Sbjct: 440 KKLGASGVLEMGNNIQAIFGTQSDALKGQIKDLINGK----EVMNDLDDQEVDETKVVAP 495

Query: 541 EPLQNEIGEEV-FVSPITGEIHPITDVPDQVFSGKMMGDGFAILPSEGIVVSPVRGKILN 599
           +    E  ++V  VSPI G++ PIT VPD+VF+ KMMGDGFAI P+EG VVSPV GKI+N
Sbjct: 496 DAQPPETAKKVELVSPIQGKLLPITQVPDKVFAEKMMGDGFAIEPTEGKVVSPVDGKIMN 555

Query: 600 VFPTKHAIGLQSDGGREILIHFGIDTVSLKGEGFTSFVSEGDRVEPGQKLLEVDLDAVKP 659
           +FPTKHAIG+ ++ G EILIH GIDTV + GEGF +FV EGD ++ GQKLLE+DL+ ++ 
Sbjct: 556 IFPTKHAIGILANSGHEILIHVGIDTVKMNGEGFETFVKEGDEIKSGQKLLEMDLNLIQE 615

Query: 660 NVPSLMTPIVFTNLAEGETVSIKASGSVNREQEDIVKIE 698
              S++TPIVFTNL E E ++++  G+V+  +  IV IE
Sbjct: 616 KASSIITPIVFTNLKEAEKITLQTEGNVDVGETGIVSIE 654


Lambda     K      H
   0.323    0.141    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1447
Number of extensions: 87
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 656
Length adjustment: 39
Effective length of query: 660
Effective length of database: 617
Effective search space:   407220
Effective search space used:   407220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources