Align ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized)
to candidate WP_078429421.1 BK574_RS17060 ABC transporter ATP-binding protein
Query= reanno::Smeli:SMc03065 (362 letters) >NCBI__GCF_002019605.1:WP_078429421.1 Length = 351 Score = 211 bits (536), Expect = 3e-59 Identities = 132/363 (36%), Positives = 196/363 (53%), Gaps = 21/363 (5%) Query: 1 MTGLLLKDIRKSYG--AVDVIHGIDLDIKEGEFVVFVGPSGCGKSTLLRMIAGLEEITGG 58 M+ + ++ K Y A + ++L+I EGE + +GPSGCGK+T LRM+AG E+ T G Sbjct: 1 MSFIQFDNVTKYYNRAATPAVDQLNLEILEGEIITLLGPSGCGKTTTLRMLAGFEQPTTG 60 Query: 59 DMFIDGERVND----VPPSKRGIAMVFQSYALYPHMTVYDNMAFGMRIARESKEEIDRRV 114 + I E V D +PP KRGI MVFQ YAL+PH+T+ N+ FG+ R + +R Sbjct: 61 KIRIGDEVVYDDRRALPPEKRGIGMVFQDYALFPHLTIEKNVTFGLN--RWKNRDKKKRA 118 Query: 115 RGAADMLQLTPYLDRLPKALSGGQRQRVAIGRAICRNPKVFLFDEPLSNLDAALRVATRI 174 + +++ L + RLP LSGGQ+QRVA+ RA+ PKV L DEP SNLDA LR R Sbjct: 119 QEVLELVGLGEFGHRLPSELSGGQQQRVALARALAPRPKVILMDEPFSNLDAGLREKMRY 178 Query: 175 EIAKLSERMSDTTMIYVTHDQVEAMTLADRIVVLSAGHIEQVGAPLELYERPANLFVARF 234 ++ + R ++ T I VTHDQ +A ++DR+VV++ G I+Q+ AP E+Y PAN FVA+F Sbjct: 179 DVTNIL-RKANATAIIVTHDQKDAFAVSDRVVVMNEGIIQQIAAPKEMYRCPANCFVAQF 237 Query: 235 IGSPAMNVIPATITATGQQTAVSLAGGKSVTLDVPTNASENGKTASFGVRPEDLRVTEAD 294 +G N+I T+ + + + +P + + +RPE R+ E Sbjct: 238 VGK--TNLISGTLCPDLKHVETHIG-----RVCLPQETDKVIENVMVSIRPEGCRLAEKG 290 Query: 295 DFLFEGTVSIVEALGEVTLLYI---EGLVENEPIIAKMPGIARVGRGDKVRFTADKAKLH 351 + G V V GE +++ V N+P+I P + G V F + Sbjct: 291 --RYAGLVERVTYSGEYQEVHVRLQSDQVSNDPMIIYAPIEQDIEIGSVVSFDITPELVA 348 Query: 352 LFD 354 L D Sbjct: 349 LVD 351 Lambda K H 0.320 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 289 Number of extensions: 14 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 362 Length of database: 351 Length adjustment: 29 Effective length of query: 333 Effective length of database: 322 Effective search space: 107226 Effective search space used: 107226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory